in vitro

Dynamique et structure neuronale

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

imaging
in vitro
Cannabinoids
remodeling in vivo

Labelisation ENP: 

2015

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

Research in my team is focused on neuronal G-protein Coupled Receptors (GPCRs). These sensory proteins are important targets oftherapeutic and abused drugs, and are usually studied by pharmacology or electrophysiology, but we assume that their functioncannot be fully understood without considering their sub-cellular environment. We have indeed found unexpectedly close relationshipbetween neuropharmacology and neuronal cell biology.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Sophie Pezet
Diana Zala
Établissements

École doctorale: 

ED3C
Laboratory

Initiatives d'Excellence: 

LabEx MemoLife
Publications

publications: 

Roland BA#, Ricobaraza A#, Carrel D#, Jordan MB, Rico F, Simon A#, Humbert-Claude M#, Ferrier J#, McFadden HM#, Scheuring S, Lenkei Z#. Cannabinoid-induced actomyosin contractility shapes neuronal morphology and growth, eLife, 2014, Sep 15;3. doi:10.7554/eLife.03159

Osmanski B, Pezet S#, Ricobaraza A#, Lenkei Z#*, Tanter M*. fUltrasound Imaging of Intrinsic Connectivity in the Living Rat Brain with High Spatiotemporal Resolution, Nature Communications, 2014, 2014 5:5023. *: co-senior authors

Thibault K#, Lin WK, Rancillac A, Fan M, Snollaerts T, Sordoillet V, Hamon M, Smith GM, Lenkei Z#, Sophie P#. BDNF-Dependent Plasticity Induced by Peripheral Inflammation in the Primary Sensory and the Cingulate Cortex Triggers Cold Allodynia and Reveals a Major Role for Endogenous BDNF As a Tuner of the Affective Aspect of Pain. J. Neurosci, 29 October 2014, 34(44):14739-14751

Ladarre D#, Roland BA#, Biedzinski S, Ricobaraza A#, Lenkei Z#. Polarized cellular patterns of endocannabinoid production and detection shape cannabinoid signaling in neurons. Frontiers in Cellular Neuroscience, 2014 (in press)

Simon AC#, Loverdo C, Gaffuri AL#, Urbanski M, Ladarre D#, Carrel D, Rivals I, Leterrier C#, Benichou O, Dournaud P, Szabo B, Voituriez R, Lenkei Z#. Activation-dependent plasticity of polarized GPCR distribution on the neuronal surface. J Mol Cell Biol. 2013 Aug;5(4):250-65.

Cortex et Epilepsie

Mots clefs: 

Epilepsie
neurone-synapse-circuit
in vitro

Labelisation ENP: 

2007

Code unité de recherche: 

UMRS 1127 UMR 7225

Cortex, Hippocampus, Epilepsie, in vitro

        Physiologie, Anatomie, Pathologie.

        Tissue epileptique humain.  Dcx, KA.

        Presubiculum, EEG, Synapse, Circuit.



Établissements

Établissement de rattachement: 

Université Pierre et Marie Curie

Établissements affiliés: 

Inserm
CNRS

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Publications

publications: 

Huberfeld G, Menendez de la Prida L, Pallud J, Cohen I, Le Van Quyen M, Adam C, Clemenceau S,  Baulac M & Miles R. (2011)  Glutamatergic preictal discharges emerge at the transition to seizure in the  human epileptic temporal lobe.  Nature Neurosci. 14: 627-34.

Bazelot M, Dinocourt C, Cohen I & Miles R (2010)  Inhibitory field potentials in the CA3 region of rat hippocampus.    J Physiology 588: 2077-90.

Motti D, Le Duigou C, Eugene E, Chemaly N, Wittner L, Lazarevic D, Krmac H, Marstrand T, Valen E, Sanges R, Stupka E, Sandelin A, Cherubini E, Gustincich S & Miles R  (2010)  Gene expression analysis of the emergence of epileptiform activity after focal injection of kainic acid into mouse hippocampus.    European J Neuroscience 32: 1364-79.

Wittner L, Huberfeld G, Clémenceau S, Erőss L, Dezamis E, Entz L, Ulbert I, Baulac M, Freund TF, Maglóczky Zs &  Miles R. (2009)  The epileptic human hippocampal CA2 region generates spontaneous interictal like activity in vitro.  Brain 132: 3032-46.          

Eugene E, Delpiel C, Baulac S, Baulac M,  Fritschy JM, LeGuern E, Miles R & Poncer JC   (2007) Synaptic and non-synaptic effects of GABRG2 mutations linked to human epileptic syndromes.  J Neurosci 27: 14108-16.