Vision

Groupe Développement des circuits neuronaux

Domaine de recherche principal: 

Neurogenetics / neurodevelopment

Mots clefs: 

neural circuits
zebrafish
Optogenetics
behavior
Vision

Labelisation ENP: 

2012

Centre de recherche / Institut: 

Institut Curie - Centre de Recherche - Paris/Orsay

Code unité de recherche: 

U934 - UMR3215

The optic tectum has emerged as a tractable visuomotor transformer, in which anatomical and functional studies can allow a better understanding of how behavior is controlled by neuronal circuits. We are examining the formation and function of the visual system in zebrafish larvae using in vivo time-lapse microscopy and state-of-the-art “connectomic” and “optogenetic” approaches to monitor and perturb neuronal activity. We apply complementary cellular and molecular analyses to dissect this circuit and identify the neuronal substrate of visual behaviors.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Karine Duroure
Thomas Auer
Christoph Gebhardt
Vincenzo Di Donato
Flavia De Santis
Celine Revenu
Shahad Albadri
Noe Testa
Établissements

Établissement de rattachement: 

Institut Curie

Établissements affiliés: 

CNRS
Inserm

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Virginie Bourgeois

Initiatives d'Excellence: 

Labex DEEP
Publications

publications: 

Dunn, T. W., Gebhardt, C., Naumann, E. A., Riegler, C., Ahrens, M. B., Engert, F., and Del Bene, F. Neural Circuits Underlying Visually Evoked Escapes in Larval Zebrafish. Neuron (2016)

Di Donato, V., De Santis, F., Auer, T. O., Testa, N., Sanchez-Iranzo, H., Mercader, N., Concordet, J. P., and Del Bene, F.  2C-Cas9: a versatile tool for clonal analysis of gene function. Genome research (2016)

Auer, T. O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J. P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., and Del Bene, F. Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLife (2015)

Auer, T. O., Duroure, K., Concordet, J. P., and Del Bene, F. CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish. Nature protocols (2014)

Auer, T. O., Duroure, K., De Cian, A., Concordet, J. P., and Del Bene, F.  Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome research (2014)

Transmission de l’information visuelle, pharmacotoxicité rétinienne et neuroprotection

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Vision
Retina
therapy
rehabilitation
blind

Labelisation ENP: 

2009

Centre de recherche / Institut: 

Institut de la Vision

Code unité de recherche: 

UMRS 968 - UMR 7210 - UM 80

La rétine réalise un véritable traitement de l’information visuelle avant son envoi au cerveau. Ce traitement des images visuelles permet notamment d’augmenter les contrastes au bord des objets et de détecter les éléments en mouvement horizontal, vertical voire en approche frontale. Cependant, les mécanismes moléculaires sous-jacents à ce traitement de l’information visuelle restent encore souvent énigmatiques alors que leur compréhension permettrait de mieux appréhender l’évolution de pathologies rétiniennes voire intervenir sur les processus neurodégénératifs.

Leader

Leader: 

Personnel

Etudiants ENP: 

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

Université Pierre et Marie Curie
CNRS
Laboratory

Initiatives d'Excellence: 

Labex Lifesenses
Publications

publications: 

Hébert C, Cottance M, Degardin J, Scorsone E, Rousseau L, Lissorgues G, Bergonzo P, Picaud S. Monitoring the evolution of boron doped porous diamond electrode on flexible retinal implant by OCT and in vivo impedance spectroscopy. Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:77-84. doi: 10.1016/j.msec.2016.06.032. Epub 2016 Jun 10.

Froger N, Moutsimilli L, Cadetti L, Jammoul F, Wang QP, Fan Y, Gaucher D, Rosolen SG, Neveux N, Cynober L, Sahel JA, Picaud S. Taurine: the comeback of a neutraceutical in the prevention of retinal degenerations. Prog Retin Eye Res. 2014 Jul;41:44-63. doi: 10.1016/j.preteyeres.2014.03.001. Epub 2014 Apr 8.

Rosolen SG, Neveux N, Sahel J A, Picaud S. Froger N IntEvaluation of the taurine concentrations in dog plasma and aqueous humour: a pilot study. Adv Exp Med Biol. (2013) 775: 145-54.

Linderholm P, Guyomard JL, Djilas M, Salzmann J, Simonutti M, Sahel JA, Safran AB, Renaud P, Picaud S. Long-term in-vivo impedance changes of subretinal microelectrodes implanted in dystrophic P23H rats. International Journal of Artificial Organs (2013) DOI:10.5301/ijao.5000213.

Amel Bendali, Lucas H. Hess, Max Seifert, Valerie Forster, Anne-Fleur Stephan, Jose A. Garrido, Serge Picaud. Purified Neurons can Survive on Peptide-Free Graphene Layers. Adv. Health. Mater. (2013) 0.1002/adhm.201200347.

Lorach H., Benosman R., Marre O., Ieng S.H., Sahel J., Picaud S. Artificial retina : the multichannel processing of the mammalian retina achieved with a neuromorphic asynchronous light acquisition device. Journal of Neural Engineering (2012) 9(6):066004.

Froger N, Cadetti L, Lorach H, Martins J, Bemelmans A, Dubus E, Dégardin J, Pain D, Forster V, Chicaud L, Ivkovic I, Simonutti M, Fouquet S, Jammoul F, Léveillard T, Benosman R, Sahel J A, Picaud S. Taurine provides neuroprotection against retinal ganglion cell degeneration. Plos One (2012) 9(6):066004.

Cognisciences: Intégration et plasticité synaptique dans le cortex visuel

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Vision
electrophysiology
imaging
Multiscale
Modeling

Labelisation ENP: 

2007

Centre de recherche / Institut: 

Unité de Neuroscience, Information et Complexité

Code unité de recherche: 

UPR 3293

An issue central to the theme of complexity in the dynamics and function of biological networks concerns the inferences that can be made from multiscale measurements ranging from microscopic to macroscopic levels of integration. Our research is based on interdisciplinary approaches ranging from electrophysiology (intracellular sharp and patch recordings, in vivo), network imaging (voltage sensitive dye), psychophysical measurements to functional databasing and phenomenological and computational modeling.

Personnel

Etudiants ENP: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Sud

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158 - 3C
Laboratory

Initiatives d'Excellence: 

Idex i-Code, Idex NeuroSaclay
Publications

publications: 

Gerard-Mercier F, Carelli PV, Pananceau M, Troncoso XG, Frégnac Y. Synaptic Correlates of Low-Level Perception in V1. J Neurosci. 2016 Apr 6;36(14):3925-42. doi: 10.1523/JNEUROSCI.4492-15.2016.

Fournier J, Monier C, Levy M, Marre O, Sári K, Kisvárday ZF, Frégnac Y. Hidden complexity of synaptic receptive fields in cat V1. J Neurosci. 2014 Apr 16;34(16):5515-28. doi: 10.1523/JNEUROSCI.0474-13.2014.

Arduin P.J. , Ego-Stengel V. , Frégnac Y. and Shulz D.E. (2013) Master neurons induced by operant conditioning in rat motor cortex during a brain-machine interface task. The Journal of Neuroscience. 33(19): 8308-20.

Levy M. , Fournier J. and Frégnac Y. (2013) The role of delayed suppression in slow and fast contrast adaptation in v1 simple cells. The Journal of Neuroscience. 33(15):6388-400.

El Boustani S. , Yger P. , Destexhe A. and Frégnac Y. (2012) A. Stable learning in stochastic network states. J. Neurosci. 32: 194-214.

Frégnac Y. (2012) Reading out the synaptic echoes of low level perception in V1. Lecture Notes in Computer Science. 7583: 486-495.

Chavane F. , Sharon D. , Jancke D. , Marre O. , Frégnac Y. and Grinvald A. (2011) Lateral spread of orientation selectivity in V1 is controlled by intracortical cooperativity. Frontiers in System Neuroscience, 5:4. 1-26.