Optogenetics

Dynamique des circuits neuronaux & comportement

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

zebrafish
neural circuits
Optogenetics
Two-photon calcium imaging
motor behaviour
cognitive functions
light-sheet microscopy
ongoing spontaneous activity

Labelisation ENP: 

2014

Centre de recherche / Institut: 

ENS Ecole Normale Supérieure

Code unité de recherche: 

U1024 UMR 8197

Using the zebrafish larva as the experimental model and a multidisciplinary approach, including twophoton calcium imaging to monitor activity of neural networks, motor behaviours, genetic engineering techniques to label, monitor and manipulate activity of specific neurons or entire circuits and mathematical methods for data analysis, we are studying the following subjects:

1) Multimodal sensory perception:

Leader

Leader: 

Établissements

Établissement de rattachement: 

ENS

Établissements affiliés: 

CNRS
Inserm

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Institut de Biologie de l'ENS IBENS

Initiatives d'Excellence: 

Labex Memolife, Idex PSL, ERC Cog 2016
Publications

publications: 

Romano SA, Pérez-Schuster V, Jouary A, Boulanger-Weill J, Candeo A, Pietri T, Sumbre G. (2017) An integrated calcium imaging processing toolbox for the analysis of neuronal population dynamics PLOS Computational Biology. 13, e1005526.

Boulanger-Weill J, Candat V, Jouary A, Romano S, Pérez-Schuster V, Sumbre G. (2017) Functional Interactions between Newborn and Mature Neurons Leading to Integration into Established Neuronal Circuits. Current Biology. 27, 1–14.

Pietri, T., Romano, S.A., Pérez-Schuster, V., Boulanger-Weill, J., Candat, V., and Sumbre G. (2017). The Emergence of the Spatial Structure of Tectal Spontaneous Activity Is Independent of Visual Inputs. Cell Reports. 19, 939–948.

Pérez-Schuster V, Kulkarni A, Nouvian M, Romano SA, Lygdas K, Jouary A, Dipoppa M, Pietri T, Haudrechy M, Candat V, Boulanger-Weill J, Hakim V, Sumbre G.  (2016) Sustained Rhythmic Brain Activity Underlies Visual Motion Perception in Zebrafish. Cell Reports. 17, 4:1098-1112.

Romano SA, Pietri T, Pérez-Schuster V, Jouary A, Haudrechy M and Sumbre G. (2015) Spontaneous neuronal network dynamics reveal circuit's functional adaptations for behavior. Neuron. 85(5):1070–1085.

 

Membrane Transport

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

Optogenetics
Transporter
Synaptic vesicle
Lysosome
Neurodegeneration

Labelisation ENP: 

2014

Centre de recherche / Institut: 

INCC - UMR 8002 - Paris Descartes University

Code unité de recherche: 

UMR 8250

RESEARCH INTERESTS:

Leader

Leader: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Descartes
Laboratory

Nom: 

Neurophotonics laboratory
Publications

publications: 

Chapel A, Kieffer-Jaquinod S, Sagné C#, Verdon Q#, Ivaldi C, Mellal M, Thirion J, Jadot M, Bruley C, Garin J, Gasnier B#, Journet  A (2013) An extended proteome map of the lysosomal membrane reveals novel potential transporters. Mol Cell Proteomics, 12:1572

M. Martineau, T. Shi, J. Puyal, A.M. Knolhoff, J. Dulong, B. Gasnier#, J. Klingauf, J. V. Sweedler, R. Jahn, and J-P Mothet (2013) Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci, 34:646.

A. Jezegou#, E. Llinares, C. Anne#, S. Kieffer-Jaquinod, S. O'Regan#, J. Aupetit , A. Chabli, C. Sagné#, C. Debacker#, B. Chadefaux-Vekemans, A. Journet, B. Andre, B. Gasnier# (2012) Heptahelical protein PQLC2 is a lysosomal cationic amino acid exporter underlying the action of cysteamine in cystinosis therapy. PNAS 109:E3434

R. Ruivo#, G.C. Bellenchi#, X. Chen#, G. Zifarelli, C. Sagné#, C. Debacker#, M. Pusch, S. Supplisson, B. Gasnier# (2012) Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin, PNAS 109:E210.

N. Pietrancosta*, C. Anne*#, H. Prescher, R. Ruivo#, C. Sagné#, C. Debacker#, H.O. Bertrand, R. Brossmer, F. Acher, B. Gasnier# (2012) Successful prediction of the substrate-binding pocket in the SLC17 transporter sialin, JBC 287:11489

Dynamiques corticales et intégration multisensorielle

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Optogenetics
multisensory processing
two photon calcium imaging
theoretical neuroscience
quantitative behavior

Labelisation ENP: 

2014

Centre de recherche / Institut: 

Unité de Neuroscience, Information et Complexité

Code unité de recherche: 

UPR 3293



Leader

Leader: 

Personnel

Etudiants ENP: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Sud

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Unité Neuroscience Information Complexité (UNIC)

Initiatives d'Excellence: 

Idex NeuroSaclay
Publications

publications: 

Thomas Deneux, Alexandre Kempf, Aurélie Daret, Emmanuel Ponsot and Brice Bathellier, Temporal asymmetries in auditory coding and perception reflect multi-layered nonlinearities, Nature Communications 7: 12682, (2016)

Yves Frégnac and Brice Bathellier, Cortical Correlates of Low-Level Perception: From Neural Circuits to Percepts, Neuron 88(1): 110-126, (2015)

Bathellier B,  Tee SP, Hrovat C, Rumpel S, A multiplicative reinforcement learning model capturing learning dynamics and inter-individual variability in mice. Proc. Natl. Acad. Sci. USA, 2013, 110(49):19950-5.

Bathellier B, Ushakova L, and Rumpel S, Spontaneous association of sounds by discrete neuronal activity patterns in the neocortex. Neuron, 2012, 76(2):435-49

INTERACTIONS ENTRE NEURONES ET OLIGODENDROGLIES DANS LA MYÉLINISATION ET LA RÉPARATION DE LA MYÉLINE

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Patch clamp
Optogenetics

Labelisation ENP: 

2014

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

Oligodendrocyte precursor cells expressing the chondroitin sulfate proteoglycan NG2, also called NG2 cells, have the ability to proliferate in the postnatal brain to generate oligodendrocytes in grey and white matters. NG2 cells play a critical role in myelination during postnatal brain development, but a pool of these progenitors is maintained in the adult and recruited to lesions in demyelinating diseases. Recent discoveries have demonstrated that NG2 cells are contacted by functional glutamatergic and GABAergic synapses from neurons in grey and white matters.

Leader

Leader: 

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

Université Paris Descartes

École doctorale: 

GC2IDED BioSPC ED572
Publications

publications: 

Balia M*, Vélez-Fort M*, Passlick S, Schäfer C, Audinat E, Steinhäuser C, Seifert G, Angulo MC (2015) Postnatal down-regulation of the GABAA receptor 2 subunit in neocortical NG2 cells accompanies synaptic-to-extrasynaptic switch in GABAergic transmission mode. Cereb Cortex, 25(4):1114-23

Balia M, Benamer N, Angulo MC (2017) A specific GABAergic synapse onto oligodendrocyte precursors does not regulate cortical oligodendrogenesis. Glia. 65(11):1821-1832.

Ledonne F, Orduz D, Mercier J, Vigier L, Grove EA, Tissir F, Angulo MC, Pierani A*, Coppola E*. (2016) Targeted inactivation of Bax reveals subtype-specific mechanism of Cajal-Retzius neuron death in the postnatal cerebral cortex. Cell Rep. 17(12) 3133

Wake H*, Ortiz FC*, Woo DH, Lee P, Angulo MC, Fields D (2015) Non-synaptic junctions on myelinating glia promote preferential myelination of electrically-active axons. Nat Commun 6:7844

Orduz D*, Maldonado PP*, Balia M, Vélez-Fort M, de Sars V, Yanagawa Y, Emiliani V, Angulo MC (2015) Interneurons and oligodendrocyte progenitors form a structured synaptic network in the developing neocortex. eLife 4:e06953

 

Transmission inhibitrice dans le cerveau

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Inhibitory transmission
Multiphoton imaging
Glycine
Vesicular loading
Optogenetics
Cerebellum

Labelisation ENP: 

2012

Centre de recherche / Institut: 

ENS Ecole Normale Supérieure

Code unité de recherche: 

U1024 - UMR8197

. La transmission synaptique inhibitrice joue un rôle central dans le fonctionnement du cerveau. Preuve en est que le blocage de l’inhibition engendre des situations pathologiques comme l’épilepsie et l’hyperekplexie, une affection rare caractérisée par des sursauts exagérés en réponse à des stimuli auditifs, visuels ou tactiles. A l’inverse, une potentialisation de l’inhibition provoque des états modifiés du cerveau comme ceux résultant de l’action de psychotropes, anxiolytiques, somnifères ou encore analgésiques.

Leader

Leader: 

Personnel

Membres de l'équipe: 

MARCAGGI Païkan
Établissements

Établissement de rattachement: 

Ecole Normale Supérieure

Établissements affiliés: 

Inserm
CNRS

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Institut de Biologie de l'ENS IBENS

Initiatives d'Excellence: 

Labex Memolife, IDEX PSL*, NIH Brain Initiative Grant
Publications

publications: 



Zampini V, Liu JK, Diana MA, Maldonado PP, Brunel N, Dieudonné S. (2016) Mechanisms and functional roles of glutamatergic synapse diversity in a cerebellar circuit. Elife. 2016 Sep 19;5. pii: e15872. doi: 10.7554/eLife.15872.

Akemann W, Léger JF, Ventalon C, Mathieu B, Dieudonné S, Bourdieu L. (2015) Fast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy. Opt Express. 23: 28191-205.

Giber K, Diana MA*, M Plattner V*, Dugué GP, Bokor H, Rousseau CV, Maglóczky Z, Havas L, Hangya B, Wildner H, Zeilhofer HU, Dieudonné S§, Acsády L. (2015) A subcortical inhibitory signal for behavioral arrest in the thalamus. Nat Neurosci. 18:562-8.

Ankri L, Husson Z*, Pietrajtis K, Proville R, Léna C, Yarom Y, Dieudonné S§, Yoe Uusisaari M. (2015) A novel inhibitory nucleo-cortical circuit controls cerebellar Golgi cell activity. Elife. 4. doi: 10.7554/eLife.06262.

Otsu Y, Marcaggi P, Feltz A, Isope P, Kollo M, Nusser Z, Mathieu B, Kano M, Tsujita M, Sakimura K, Dieudonné S§. (2014) Activity-dependent gating of calcium spikes by A-type K+ channels controls climbing fiber signaling in Purkinje cell dendrites. Neuron. 84 : 137-51.

Groupe Développement des circuits neuronaux

Domaine de recherche principal: 

Neurogenetics / neurodevelopment

Mots clefs: 

neural circuits
zebrafish
Optogenetics
behavior
Vision

Labelisation ENP: 

2012

Centre de recherche / Institut: 

Institut Curie - Centre de Recherche - Paris/Orsay

Code unité de recherche: 

U934 - UMR3215

The optic tectum has emerged as a tractable visuomotor transformer, in which anatomical and functional studies can allow a better understanding of how behavior is controlled by neuronal circuits. We are examining the formation and function of the visual system in zebrafish larvae using in vivo time-lapse microscopy and state-of-the-art “connectomic” and “optogenetic” approaches to monitor and perturb neuronal activity. We apply complementary cellular and molecular analyses to dissect this circuit and identify the neuronal substrate of visual behaviors.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Karine Duroure
Thomas Auer
Christoph Gebhardt
Vincenzo Di Donato
Flavia De Santis
Celine Revenu
Shahad Albadri
Noe Testa
Établissements

Établissement de rattachement: 

Institut Curie

Établissements affiliés: 

CNRS
Inserm

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Virginie Bourgeois

Initiatives d'Excellence: 

Labex DEEP
Publications

publications: 

Dunn, T. W., Gebhardt, C., Naumann, E. A., Riegler, C., Ahrens, M. B., Engert, F., and Del Bene, F. Neural Circuits Underlying Visually Evoked Escapes in Larval Zebrafish. Neuron (2016)

Di Donato, V., De Santis, F., Auer, T. O., Testa, N., Sanchez-Iranzo, H., Mercader, N., Concordet, J. P., and Del Bene, F.  2C-Cas9: a versatile tool for clonal analysis of gene function. Genome research (2016)

Auer, T. O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J. P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., and Del Bene, F. Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLife (2015)

Auer, T. O., Duroure, K., Concordet, J. P., and Del Bene, F. CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish. Nature protocols (2014)

Auer, T. O., Duroure, K., De Cian, A., Concordet, J. P., and Del Bene, F.  Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome research (2014)

Spinal Sensory Signalling

Domaine de recherche principal: 

Computational neurosciences / neural theory

Mots clefs: 

behavior
Optogenetics
Locomotion
Circuit-breaking
Spinal cord

Labelisation ENP: 

2010

Centre de recherche / Institut: 

Institut du Cerveau et de la Moelle épinière

Code unité de recherche: 

UMRS 1127 UMR 7225

Les systèmes sensoriels transforment les fluctuations du monde physique en séquences de potentiels d'action qui sont intégrés pour contrôler les sorties motrices. La locomotion repose sur des circuits composés par des interneurones spinaux et capables de générer des oscillations. La physiologie moderne a permis d'établir la connectivité entre certaines cellules sensorielles et interneurones, mais la plupart des études se sont limitées à des préparations paralysées où les entrées locales ne peuvent être actives.

Leader

Leader: 

Personnel

Etudiants ENP: 

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

CNRS
Université Pierre et Marie Curie

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Initiatives d'Excellence: 

IHU-A-ICM, ERC Grant 2013-18
Publications

publications: 

Knafo S#, Wyart C@ [2017]. Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae. eLife, in press.

Knafo S#,%, Fidelin K#,%, Prendergast A+, Tseng PE, Parrin A, Dickey CW, Bohm UL#, Nunes Figueiredo S, Thouvenin O, Pascal-Moussellard H, Wyart C@ [2017]. Mechanosensory neurons control the timing of spinal microcircuit selection during locomotion. eLife 6:e25260 DOI: 10.7554/eLife.25260

 

Djenoune L#, Desban L#, Gomez J, Sternberg JR#, Prendergast A+, Langui D, Quan FB#, Marnas H#, Auer TO, Rio JP, Del Bene F, Bardet PL@, Wyart C@ [2017]. The dual developmental origin of spinal cerebrospinal fluid-contacting neurons gives rise to distinct functional subtypes, Scientific Reports 7:719.

Hubbard J+, Böhm U#, Prendergast A+, Tseng PE, Stokes C+, Newman M, Wyart C@ [2016]. GABAergic sensory neurons project onto key elements of the escape circuit, Current Biology 26: 2841-2853.

Sternberg J#,%, Severi K+,%, Fidelin K, Gomez J, Ihara H, Alcheikh Y, Hubbard J, Kawakami K, Suster M, Wyart C@[2016]. Optimization of Botulinum toxin to probe the role of specific interneurons in innate locomotion. Current Biology, 26: 2319-28.

Réseau Cortical et Couplage Neurovasculaire

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

cerebral cortex
electrophysiology
Optogenetics
neuronal circuits
blood perfusion and metabolism

Labelisation ENP: 

2007

Code unité de recherche: 

U1130 UMR8246 UMCR18

The team “Cortical network and Neurovascular Coupling” studies neuronal circuits and their relationships with the glio-vascular network in the cerebral cortex. Our previous work has advanced our knowledge of neocortical neuronal types, of their sensitivity to afferent signals, of their places in the functional architecture of intracortical peptidergic transmission, and of their differential roles in neurovascular coupling.

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

CNRS
Université Pierre et Marie Curie

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Neuroscience Paris Seine

Initiatives d'Excellence: 

IHU Institut de Neurosciences Translationnelles de Paris
Publications

publications: 

Lecrux C, Toussay X, Kocharyan A, Fernandes P, Neupane S, Lévesque M, Plaisier F, Shmuel A, Cauli B, Hamel E. Pyramidal neurons are "neurogenic hubs" in the neurovascular coupling response to whisker stimulation. J Neurosci. 2011 Jul 6;31(27):9836-47.

Hu E, Demmou L, Cauli B, Gallopin T, Geoffroy H, Harris-Warrick RM, Paupardin-Tritsch D, Lambolez B, Vincent P, Hepp R. VIP, CRF, and PACAP act at distinct receptors to elicit different cAMP/PKA dynamics in the neocortex. Cereb  Cortex. 2011 Mar;21(3):708-18.

Tricoire L, Pelkey KA, Daw MI, Sousa VH, Miyoshi G, Jeffries B, Cauli B, Fishell G, McBain CJ. Common origins of hippocampal Ivy and nitric oxide synthase expressing neurogliaform cells. J Neurosci. 2010 Feb 10;30(6):2165-76.

Karagiannis A, Gallopin T, Dávid C, Battaglia D, Geoffroy H, Rossier J, Hillman EM, Staiger JF, Cauli B. Classification of NPY-expressing neocortical interneurons. J Neurosci. 2009 Mar 18;29(11):3642-59.

Petilla Interneuron Nomenclature Group, Ascoli GA, Alonso-Nanclares L, Anderson SA, Barrionuevo G, Benavides-Piccione R, Burkhalter A, Buzsáki G, Cauli  B, Defelipe J, Fairén A, Feldmeyer D, Fishell G, Fregnac Y, Freund TF, Gardner D, Gardner EP, Goldberg JH, Helmstaedter M, Hestrin S, Karube F, Kisvárday ZF, Lambolez B, Lewis DA, Marin O, Markram H, Muñoz A, Packer A, Petersen CC, Rockland KS, Rossier J, Rudy B, Somogyi P, Staiger JF, Tamas G, Thomson AM, Toledo-Rodriguez M, Wang Y, West DC, Yuste R. Petilla terminology: nomenclature of features of GABAergic interneurons of the cerebral cortex. Nat Rev Neurosci. 2008 Jul;9(7):557-68.