Neurodegeneration

Gènes Circuits Rythmes et Neuropathologies

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

dopamine
Neurodegeneration
Parkinson disease
Locomotor aging
Drosophila
oxidative stress

Labelisation ENP: 

2016

Centre de recherche / Institut: 

ESPCI Paris Tech -Ecole Supérieure de Physique et de Chimie Industrielles

Code unité de recherche: 

UMR 8249

Our research program is focused on the role of dopamine in behaviour control and neurodegenerative mechanisms in the fruit fly Drosophila melanogaster. Our approach combines Drosophila genetics, molecular biology, brain imaging and behavioral assays. We recently generated and characterized brain dopamine-deficient mutants and developed new models to study pathogenic mechanisms related to genetic and environmental factors of Parkinson disease.

Leader

Leader: 

Personnel

Membres de l'équipe: 

André Klarsfeld
Baya Chérif-Zahar
Ali Mteyrek
Abdul-Raouf Issa
Alexandra Vaccaro
Mathilde Lecompte
Jun Sun
Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

ESPCI

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED 158 3C
Laboratory

Nom: 

Laboratoire Plasticité du Cerveau

Initiatives d'Excellence: 

Labex MemoLife, Idex PSL
Publications

publications: 

Cassar M., Issa A.-R., Riemensperger T., Petitgas C., Rival T., Coulom H., Iché-Torres M., Han K.-A. & Birman S. (2015) A dopamine receptor contributes to paraquat-induced neurotoxicity in Drosophila. Hum. Mol. Genet. 24(1):197-212

Lavista-Llanos S., Svatoš A., Kai M., Riemensperger T., Birman S., Stensmyr M. C. & Hansson B. S. (2014) Dopamine drives Drosophila sechellia adaptation to its toxic host. eLife Dec 9;3

Riemensperger T., Issa A.-R., Pech U., Coulom H., Nguyen M.-V., Cassar M., Jacquet M., Fiala A. & Birman S. (2013) A single dopamine pathway underlies progressive locomotor deficits in a Drosophila model of Parkinson disease. Cell Rep. 5:952–960

Humphrey D. M., Parsons R. B., Ludlow Z. N., Riemensperger T., Esposito G., Verstreken P., Jacobs H. T., Birman S. & Hirth F. (2012) Alternative oxidase rescues mitochondria-mediated dopaminergic cell loss in Drosophila. Hum. Mol. Genet. 21(12): 2698-2712

Riemensperger T., Isabel G., Coulom H., Neuser K., Seugnet L., Kume K., Iché-Torres M., Cassar M., Strauß R., Préat T., Hirsh J. & Birman S. (2011) Behavioral consequences of dopamine deficiency in the Drosophila central nervous system. Proc. Natl. Acad. Sci. USA 108(2):834-39 



Régulation de la dynamique des microtubules et de leurs fonctions

Domaine de recherche principal: 

Neurogenetics / neurodevelopment

Mots clefs: 

Neurodegeneration
tubulin code
microtubule cytoskeleton
posttranslational modifications
neuronal differentiation

Labelisation ENP: 

2015

Centre de recherche / Institut: 

Institut Curie - Centre de Recherche - Paris/Orsay

Code unité de recherche: 

UMR3348

In our ongoing projects, we are using biochemistry, biophysics and structural biology in conjunction with cell and mouse biology to identify the molecular mechanisms by which tubulin posttranslational modifications regulative microtubule behaviour and functions, and which are the cellular and developmental roles of these modifications and the corresponding enzymes. Our functional studies are focussed on the nervous system, cilia and flagella (including spermatogenesis), and cell division.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Annemarie Wehenkel
Cecilia Serieyssol
Kathiresan Natarajan
Laurence Vaslin
Maria Magiera
Ngoc-Lan Julie Nguyen
Sudarshan Gadadhar
Tiziana Giordano
Lurlene Akendengue
Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Institut Curie
Université Paris Sud 11

Université: 

Université Paris Saclay / Université Paris Descartes

École doctorale: 

SDSV / FdV
Laboratory

Initiatives d'Excellence: 

Labex CelTisPhyBio, contrat ANR, équipe FRM
Publications

publications: 

Gadadhar S, Dadi H, Bodakuntla S, Schnitzler A, Bieche I, Rusconi F, Janke C (2017) Tubulin glycylation controls primary cilia length. J Cell Biol 216: 2701-2713

Barisic M, Silva e Sousa R, Tripathy SK, Magiera MM, Zaytsev AV, Pereira AL, Janke C, Grishchuk EL, Maiato H (2015) Microtubule detyrosination guides chromosomes during mitosis. Science 348: 799-803

Nirschl JJ, Magiera MM, Lazarus JE, Janke C, Holzbaur ELF (2016) alpha-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons. Cell Rep: celrep 2509

Belvindrah R, Natarajan K, Shabajee P, Bruel-Jungerman E, Bernard J, Goutierre M, Moutkine I, Jaglin XH, Savariradjane M, Irinopoulou T, Poncer J-C, Janke C, Francis F (2017) Mutation of the alpha-tubulin Tuba1a leads to straighter microtubules and perturbs neuronal migration. J Cell Biol 216: 2443-2461

Bosch Grau M, Masson C, Gadadhar S, Rocha C, Tort O, Marques Sousa P, Vacher S, Bieche I, Janke C (2017) Alterations in the balance of tubulin glycylation and glutamylation in photoreceptors leads to retinal degeneration. J Cell Sci 130: 938-949

 

Membrane Transport

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

Optogenetics
Transporter
Synaptic vesicle
Lysosome
Neurodegeneration

Labelisation ENP: 

2014

Centre de recherche / Institut: 

INCC - UMR 8002 - Paris Descartes University

Code unité de recherche: 

UMR 8250

RESEARCH INTERESTS:

Leader

Leader: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Descartes
Laboratory

Nom: 

Neurophotonics laboratory
Publications

publications: 

Chapel A, Kieffer-Jaquinod S, Sagné C#, Verdon Q#, Ivaldi C, Mellal M, Thirion J, Jadot M, Bruley C, Garin J, Gasnier B#, Journet  A (2013) An extended proteome map of the lysosomal membrane reveals novel potential transporters. Mol Cell Proteomics, 12:1572

M. Martineau, T. Shi, J. Puyal, A.M. Knolhoff, J. Dulong, B. Gasnier#, J. Klingauf, J. V. Sweedler, R. Jahn, and J-P Mothet (2013) Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci, 34:646.

A. Jezegou#, E. Llinares, C. Anne#, S. Kieffer-Jaquinod, S. O'Regan#, J. Aupetit , A. Chabli, C. Sagné#, C. Debacker#, B. Chadefaux-Vekemans, A. Journet, B. Andre, B. Gasnier# (2012) Heptahelical protein PQLC2 is a lysosomal cationic amino acid exporter underlying the action of cysteamine in cystinosis therapy. PNAS 109:E3434

R. Ruivo#, G.C. Bellenchi#, X. Chen#, G. Zifarelli, C. Sagné#, C. Debacker#, M. Pusch, S. Supplisson, B. Gasnier# (2012) Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin, PNAS 109:E210.

N. Pietrancosta*, C. Anne*#, H. Prescher, R. Ruivo#, C. Sagné#, C. Debacker#, H.O. Bertrand, R. Brossmer, F. Acher, B. Gasnier# (2012) Successful prediction of the substrate-binding pocket in the SLC17 transporter sialin, JBC 287:11489