light-sheet microscopy

Functional imaging and behavior in zebrafish

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

light-sheet microscopy
neuroimaging
zebrafish
sensory-motor integration
circuit inference

Labelisation ENP: 

2016

Centre de recherche / Institut: 

Laboratoire Jean Perrin

Code unité de recherche: 

UMR 8237

The overall objective of the group is to unravel principles of neural computations underlying sensory-motor integration in the vertebrate brain. We use the zebrafish larva as it currently constitutes the only vertebrate system amenable to whole-brain recording with cellular resolution. Using one- or two-photon light-sheet microscopy, we are able to monitor the long-term activity of the quasi-entirety of the 100,000 neurons that comprise the animal brain, as it performs basic sensory-motor tasks.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Raphaël Candelier
Sergei Kruglik
Sébastien Wolf
Alexis Dubreuil
Sophia Karpenko
Guillaume Le Goc
Établissements

Établissement de rattachement: 

UPMC

Établissements affiliés: 

CNRS

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED 564
Publications

publications: 

Sensorimotor Computation underlying phototaxis in zebrafish. Sébastien Wolf, Alexis M. Dubreuil, Tommaso Bertoni, Urs Lucas Böhm, Volker Bormuth, Raphaël Candelier, Sophia Karpenko, David G. C. Hildebrand, Isaac H. Bianco, Rémi Monasson, Georges Debrégeas. Nature Communication, in press

Whole-brain functional imaging with two-photon light-sheet microscopy, S Wolf, W. Supatto, G. Debrégeas, P. Mahou, S. G Kruglik,J-M. Sintes, E. Beaurepaire, R. Candelier Nature Methods 12, 379–380 (2015)

An Amplitude Modulation/Demodulation Scheme for Whisker-Based Texture Perception, Y. Boubenec, L. N. Claverie, D. E. Shulz,G. Debrégeas, J. Neurosci. 34(33): 10832-10843 (2014)

Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy. T. Panier,S. Romano, R. Olive, T. Pietri, G. sumbre, R. Candelier, G. Debrégeas, Frontiers in Neural Circuits 7, 0065 (2013)

The role of fingerprints in the coding of tactile information probed with a biomimetic sensor, J. Scheibert, S. Leurent, A. Prevost et G. Debrégeas, Science 323 1503-1506 (2009). Reviewed in Science news, Science Update (radio), Science signaling, Nature news, Nature Research highlights, Nature Methods,Analytical Chemistry, Atten. Percept & Psych.

Signatures of granular microstructure in dense shear flows. D. Mueth, G. Debregeas, G. Karczmar, P. Eng, S. Nagel & H. Jaeger,Nature 406, 385-388 (2000)

Multisensory signal processing: From whole-brain activity to behavior

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Labelisation ENP: 

2016

Centre de recherche / Institut: 

Laboratoire Jean Perrin

Code unité de recherche: 

UMR 8237

Our brain needs to constantly fuse sensory information detected by our multiple senses in order to produce a seamless coherent representation of the world. Rather than being the exception, this binding process is ubiquitous to sensory-motor integration and is implicated in most cognitive functions. Its impairment is a cause of various pathologies, such as schizophrenia or autism. Multisensory processing operates on all brain levels from primary cortices over subcortical structures up to higher associative centers, while the smallest operational units are single multisensory neurons.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Thomas Panier (PostDoc)
Kris Severi (PostDoc)
Geoffrey Migault (PhD)
Hugo Trentenseaux (PhD)
Matthieu Dujany (Master)
Louis Chevalier (Master)
Établissements

Établissement de rattachement: 

Sorbonne Université

Établissements affiliés: 

CNRS

Université: 

UPMC

École doctorale: 

ED 564
Publications

publications: 

Bormuth, V., Barral, J., Joanny, J.F., Jülicher, F. & Martin, P.    Transduction channels gating controls friction on vibrating hair-cell bundles in the ear.

PNAS 111, 7185-90 (2014) 

Gardner, M.K.*, Zanic, M.*, Gell, C., Bormuth, V., & Howard J.    Depolymerizing Kinesins Kip3 and MCAK Shape
Cellular Microtubule Architecture by Differential Control of Catastrophe.

CELL 147, 1092-1103 (2011)

Bormuth, V., Varga, V., Howard, J., & Schäffer, E.    Protein friction limits diffusive and directed movements of kinesin motors on microtubules.

SCIENCE 325, 870 - 873 (2009)

Varga, V.*, Leduc, C.*, Bormuth, V., Diez, S., & Howard, J.    inesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization.

CELL 138, 1174-83 (2009) 

Bormuth, V.*, Otsu, Y.*, et al.      Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope.

J. Neurosci. Methods 173(2), 259-270 (2008)   

Dynamique des circuits neuronaux & comportement

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

zebrafish
neural circuits
Optogenetics
Two-photon calcium imaging
motor behaviour
cognitive functions
light-sheet microscopy
ongoing spontaneous activity

Labelisation ENP: 

2014

Centre de recherche / Institut: 

ENS Ecole Normale Supérieure

Code unité de recherche: 

U1024 UMR 8197

Using the zebrafish larva as the experimental model and a multidisciplinary approach, including twophoton calcium imaging to monitor activity of neural networks, motor behaviours, genetic engineering techniques to label, monitor and manipulate activity of specific neurons or entire circuits and mathematical methods for data analysis, we are studying the following subjects:

1) Multimodal sensory perception:

Leader

Leader: 

Établissements

Établissement de rattachement: 

ENS

Établissements affiliés: 

CNRS
Inserm

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Institut de Biologie de l'ENS IBENS

Initiatives d'Excellence: 

Labex Memolife, Idex PSL, ERC Cog 2016
Publications

publications: 

Romano SA, Pérez-Schuster V, Jouary A, Boulanger-Weill J, Candeo A, Pietri T, Sumbre G. (2017) An integrated calcium imaging processing toolbox for the analysis of neuronal population dynamics PLOS Computational Biology. 13, e1005526.

Boulanger-Weill J, Candat V, Jouary A, Romano S, Pérez-Schuster V, Sumbre G. (2017) Functional Interactions between Newborn and Mature Neurons Leading to Integration into Established Neuronal Circuits. Current Biology. 27, 1–14.

Pietri, T., Romano, S.A., Pérez-Schuster, V., Boulanger-Weill, J., Candat, V., and Sumbre G. (2017). The Emergence of the Spatial Structure of Tectal Spontaneous Activity Is Independent of Visual Inputs. Cell Reports. 19, 939–948.

Pérez-Schuster V, Kulkarni A, Nouvian M, Romano SA, Lygdas K, Jouary A, Dipoppa M, Pietri T, Haudrechy M, Candat V, Boulanger-Weill J, Hakim V, Sumbre G.  (2016) Sustained Rhythmic Brain Activity Underlies Visual Motion Perception in Zebrafish. Cell Reports. 17, 4:1098-1112.

Romano SA, Pietri T, Pérez-Schuster V, Jouary A, Haudrechy M and Sumbre G. (2015) Spontaneous neuronal network dynamics reveal circuit's functional adaptations for behavior. Neuron. 85(5):1070–1085.