Regulation of myelin repair by glutamatergic transmission using an electropysiological and optogenetic approach
Dates:1 September 2016 - 1 December 2016
Date limite de candidature:1 September 2016
Lab rotation proposal: 3 months
~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017
Address: Neurophysiology & New Microscopies Laboratory - 45, rue des Saints Pères 75006 Paris
Phone number: +33 1 70 64 99 35 ; Email: firstname.lastname@example.org
Oligodendrocyte precursor cells (or NG2 cells) constitute a major source of remyelinating oligodendrocytes in demyelinating diseases. Recent advances have shown that these non-neuronal cells are contacted by through glutamatergic synapses from neurons, but the role of these unique neuron-glia synapses of the brain is unknown. It has been suggested that glutamatergic synaptic transmission onto NG2 cells might regulate proliferation and differentiation of these cells, and thus possibly impact myelination and myelin repair (two fundamental processes needed to improve action potential conduction). In this project, we will evaluate the effect of neuronal activity on myelin repair in a mouse model of demyelination. We will combine patch-clamp recordings with a novel optogenetic approach allowing for targeted stimulation of axons with light.