Neurogénétique du Poisson Zébré

Leader

Research center

25 rue du Docteur Roux
75015 Paris
Christian Bréchot

Institution

CNRS
ED 515 Complexité du Vivant
Université Pierre et Marie Curie

Laboratory

Department of Developmental and stem cell Biology
UMR3738
Labex Revive

Mots clefs

zebrafish
behavior
motivation
neurogenesis
stem cell
telencephalon
quiescence
 

publications

//Galant S, Furlan G, Coolen M, Dirian L, Foucher I, Bally-Cuif L. Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain. Dev Biol. 2016 Dec 1;420(1):120-135. doi:10.1016/j.ydbio.2016.09.022. 

//J.W. von Trotha#, P. Vernier and L. Bally-Cuif#. Emotions and motivated behavior converge on an amygdala-like structure in thezebrafish. Eur. J. Neurosc., Aug 22. doi: 10.1111/ejn.12692 (2014).E. Salta, P. Lau, C. Sala Frigerio, M. Coolen#, L. Bally-Cuif# and B. De Strooper. A self-organizing miR-132/Ctbp2 circuit regulatesbimodal Notch signals and progenitor fate choice during spinal cord maturation. Dev. Cell 30:423-436 (2014).

//A. Bosco#, C. Bureau, P. Affaticati, P. Gaspar, L. Bally-Cuif#, and C. Lillesaar#. Development of hypothalamic serotoninergicneurons in zebrafish requires Fgf-signaling acting via the ETS-domain transcription factor Etv5b (Erm). Development 140 : 372-384(2013).

//J.W. von Trotha#, P. Vernier and L. Bally-Cuif#. Emotions and motivated behavior converge on an amygdala-like structure in thezebrafish. Eur. J. Neurosc., Aug 22. doi: 10.1111/ejn.12692 (2014).E. Salta, P. Lau, C. Sala Frigerio, M. Coolen#, L. Bally-Cuif# and B. De Strooper. A self-organizing miR-132/Ctbp2 circuit regulatesbimodal Notch signals and progenitor fate choice during spinal cord maturation. Dev. Cell 30:423-436 (2014).

//L. Dirian#, S. Galant#, M. Coolen#, J. Livet, W. Chen, S. Bedu#, C. Houart, L. Bally-Cuif#* and I. Foucher#*. Spatial regionalizationand heterochrony in the formation of adult pallial neural stem cells. Dev. Cell 30:123-136 (2014). * co-senior authorship.

//M. Lange#, F. Neuzeret, B. Fabrèges, C. Froc#, S. Bedu#, L. Bally-Cuif#* and WHJ Norton#*. Inter-individual and inter-strainvariations in zebrafish locomotor ontogeny. PLOS One 8:e70172 (2013). * co-senior authorship.

//A. Alunni#, M. Krecsmarik#, A. Bosco#, S. Galant#, L. Pan, C.B. Moens and L. Bally-Cuif#. Notch 3 signaling gates cell cycle entryand limits neural stem cell amplification in the adult pallium. Development, 140:3335-3347 (2013).

Fields of research

Neurogenetics / neurodevelopment

Research Theme

Le système nerveux central des vertébrés est un assemblage complexe de neurones et de cellules gliales, mis en place en nombres et positions précis, principalement au cours du développement. Le maintien de progéniteurs neuraux jusqu’à des stades plus avancés et même jusqu’à l’âge adulte est cependant crucial, puisque ces progéniteurs pourront être utilisés dans des événements adaptatifs tardifs. Dans ce contexte, notre but est d’identifier les mécanismes impliqués dans le contrôle de la neurogenèse et de la spécification neuronale dans le cerveau embryonnaire et adulte, et leur influence sur le comportement.

Nous utilisons le système modèle « poisson zébré » (Danio rerio, zebrafish), qui permet de manipuler l’expression de gènes au cours du développement et chez l’adulte, qui maintient un grand nombre de cellules souches neurales adultes, et qui se prête également à des tests comportementaux variés.

Renouvellement 2014

Etudiants ENP

Shauna KATZ

Membres de l'équipe

Alessandro Alunni
Marion Coolen

Lab rotation

Nuclear Function of microRNAs in Neural Stem Cell Quiescence

Chercheur responsable: 

BALLY-CUIF Laure

Dates: 

2 January 2018 - 29 June 2018

Date limite de candidature: 

29 June 2018

Period

~ Jan-March 2018

~ April-June 2018

Project

Adult neurogenesis is the generation of new neurons and glial cells from neural stem cells (NSCs) present in the adult brain. Fundamentally, this process requires a fine balance between maintaining a quiescent NSC reserve and recruiting them into the neurogenesis cascade. However our knowledge the safeguarding molecular pathways involved is far from comprehensive. Exploiting an emerging model system, the zebrafish, we recently identified an unexpected nuclear activity of microRNAs in maintaining a sub-state of NSC quiescence that emerges only at adult stages. Using these findings as an entry point, the research project aims at uncovering the molecular mechanism of action of nuclear microRNAs, and better understand the diversity of quiescent states in NSCs and their establishment during post-embryonic development.

Contact

Institut Pasteur - 25, rue du docteur Roux 75015 Paris - +33 1 44 38 95 70 - marion.coolen@pasteur.fr ; laure.bally-cuif@pasteur.fr

Superviseur: 

COOLEN Marion