Mice, Molecules and Synapse formation

Leader

Research center

11 place Marcelin Berthelot
75231 Paris
Serge Haroche

Institution

Collège de France
CNRS
Inserm
ED158
Université Pierre et Marie Curie

Laboratory

Center for Interdisciplinary Research in Biology
Phone: 01 44 27 16 75
UMR 7241 - U1050
Labex Memolife Idex PSL*

Mots clefs

Synapses, molecules, transgenesis, mice, development
Available to host a PhD student

publications

Usardi A, Iyer K, Sigoillot SM, Dusonchet A, Selimi F. The immunoglobulin-like superfamily member IGSF3 is a developmentally regulated protein that controls neuronal morphogenesis. Dev Neurobiol. 2016 Jun 21. doi: 10.1002/dneu.22412.

Sigoillot S.M., Iyer K., Binda F., González-Calvo I., Talleur M., Vodjdani G., Isope P., SELIMI F. The Secreted Protein C1QL1 and Its Receptor BAI3 Control the Synaptic Connectivity of Excitatory Inputs Converging on Cerebellar Purkinje Cells. Cell Reports 2015, Feb 4. pii: S2211-1247(15)00059-5.

Proville R., Spolidoro M., Guyon N., Selimi F., Isope P., Popa D., Léna C. Interaction of sensory, motor and cerebellar cortices in the control of voluntary movements. Nat Neurosci. 2014, Sep;17(9):1233-9.

 ChaumontJ., GuyonN., ValeraA., DuguéG.P., PopaD., MarcaggiP., GautheronV., Reibel-FoissetS., DieudonnéS., StephanA., BarrotM., CasselJC., DupontJL., DoussauF., PoulainB., SELIMI F.*, Léna C.*, Isope P.* Clusters of cerebellar Purkinje cells control their afferent climbing fiber discharge. Proc. Natl. Acad. Sci. USA, (2013) 110(40):16223-16228. *equal contribution.

 Lanoue V., Usardi A., Sigoillot S.M., Talleur M., Iyer K., Mariani J., Isope P., Vodjdani G., Heintz N., SELIMI F. The adhesion‐GPCR BAI3, a gene linked to psychiatric disorders, regulates dendrite morphogenesis in neurons. Molecular Psychiatry (2013) 18(8):943-50.

 Heller E.A., Zhang W., SELIMI F., Earnheart JC., Slimak M., Santos-Torres J., Ibanez-Tallon I., Aoki C., Chait B.T., Heintz N. The biochemical anatomy of cortical inhibitory synapses. PLoS One, (2012) vol. 7, no.6, e39572.

SELIMI F., Cristea I.M., Heller E., Chait B.T., HeintzN. Proteomic studies of a single CNS synapse type: specific regulatory components of the parallel fiber/Purkinje cell synapse. PloS Biology (2009) 7(4): e83. 

Fields of research

Neurogenetics / neurodevelopment

Research Theme

The brain is composed of many different types of neuronal populations that form functional networks by establishing specific synapses. Any disturbance in the process leading to the development of these networks can contribute to neurological diseases such as autism or schizophrenia. This complex process involves both target recognition, through a putative “molecular code”, and activity-dependent stabilization/elimination of synapses. Our scientific goal is to provide new insights on the formation of functional neural networks in vivo, in particular through identification of the genes and proteins controlling synapse specificity. In the past few years, we have developed the synaptic protein profiling approach, which enables, for the first time, the purification of a specific type of synapse from the mouse brain and identification of its protein content. The recently described bacTRAP approach allows gene expression studies in specific neuronal populations in mice. Combining these two innovative strategies and applying them to the study of the olivo-cerebellar network, the specific aims of my project are to: 1) perform the first comparison of the protein content of two types of synapses made on the same neuronal target, to identify the proteins characterizing the putative “molecular synaptic code”; 2) study the function of the genes identified in our analysis and their potential contribution to brain diseases, by performing a systematic analysis of their function using expression knockdown.

Read now in the Public section of the ENP website:  "A l'origine des Maladies Psychiatriques: Des problèmes de connexion dans les réseaux neuronaux du cerveau ?"

Etudiants ENP

Keerthana IYER