Inhibition synaptique et auto-régulation des microcircuits corticaux

Leader

Research center

47 bld de l'Hôpital
75651 Paris
Alexis Brice

Institution

Inserm
CNRS
Université Pierre et Marie Curie
ED158
Université Pierre et Marie Curie

Laboratory

UMRS 1127 UMR 7225
LabEX BioPsy, ENI-Net

Mots clefs

Inhibition
cerebral cortex
interneurons
synaptic transmission
GABA
 

publications

Antonucci F, Corradini I, Morini R, Fossati G, Menna E, Pozzi D, Pacioni S, Verderio C, Bacci A, Matteoli M (2013) Reduced SNAP-25 alters short-term plasticity at developing glutamatergic synapses EMBO Rep. 14:645-51.

Baptista S, Lourenço J, Milhazes N, Borges F, Silva AP, Bacci A. Long-Term Treatment with Low Doses of Methamphetamine Promotes Neuronal Differentiation and Strengthens Long-Term Potentiation of Glutamatergic Synapses onto Dentate Granule Neurons. eNeuro. 2016 Jul 11;3(3). pii: ENEURO.0141-16.2016. doi: 10.1523/ENEURO.0141-16.2016. eCollection 2016 May-Jun.

Lourenço J, Pacioni S, Rebola N, van Woerden GM, Marinelli S, DiGregorio D, Bacci A (2014) Non-associative potentiation ofperisomatic inhibition alters the temporal coding of neocortical layer 5 pyramidal neurons PLoS Biology 12:e1001903

Fields of research

Neurophysiology / systems neuroscience

Research Theme

In the cerebral cortex the constant computation of incoming sensory information is dynamically integrated to provide a coherent representation of the world and generate highly sophisticated cognitive functions. Cortical circuits are made of different neuron types connecting one another through a staggering number of synaptic connections that are responsible for the propagation of information between neurons. The result is the generation of complex functional networks, whose specific activities often produce a wide range of synchronous rhythms, believed to provide the computational substrate for different aspects of cognition. In this context, a tight balance between excitation and inhibition is fundamental for correct brain functioning, as serious neurological and psychiatric diseases can develop when this equilibrium is altered.

Among all cell types, inhibitory cortical neurons (also known as interneurons, which use GABA as neurotransmitter) are highly heterogeneous. In particular, we are focused on (i) how different types of neurons of the cerebral cortex connect one another; (ii) how specific cell types produce different forms of synaptic transmission and plasticity; and (iii) how specific synaptic properties contribute generating various forms of network oscillations. Indeed, GABAergic neurotransmission is fundamental for integrating and filtering incoming information as well as for dictating postsynaptic neuronal spike timing, therefore providing a tight temporal code used by each neuron, or ensemble of neurons, to perform sophisticated computational operations. Altogether, results of these experiments will lead to a better understanding of GABAergic interneuron regulation of neocortical excitability, relevant to both normal and pathological cortical function.

Etudiants ENP

Cristina DONATO

Lab rotation

Cellular Physiology of Cortical Microcircuits

Chercheur responsable: 

BACCI Alberto

Dates: 

1 September 2016 - 1 December 2016

Date limite de candidature: 

1 December 2016

Lab rotation proposal: 3 months 

~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017   

AddressInstitut Du Cerveau et de la Moelle Épinière - 47 bld de l'Hôpital 75651 Paris

Phone number: +33 1 57 27 40 62 ; Emailalberto.bacci@icm-institute.org

Website

Students will be involved in studies on synaptic transmission and plasticity between different elements of the cortical microcircuit. Students will learn multiple-patch clamp recordings in cortical slices,  imaging, optogenetics, in vivo gene delivery via viral vectors and in utero electroporation, stereotaxic surgery, neuroanatomy, cell biology, immunohistochemistry.

Superviseur: 

Alberto BACCI