Coordination of morphogenesis with axonogenesis in the developing olfactory placode
Dates:1 September 2016 - 30 June 2017
Date limite de candidature:1 September 2016
~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017
Drosophila has about 150 clock neurons that control time-of-day information and orchestrate circadian behavioral rhythms such as sleep-wake cycles. Different periods of diurnal time, e.g., morning and evening, are encoded by distinct neuronal subpopulations. We are interested in (a) deciphering the pathway of information flow from these master clock neurons, (b) and in understanding how the downstream circuit decodes temporal information. To this end we have selected transgenic lines that express Gal4/LexA transgenes in specific populatins of neurons whose anatomical location in the brain suggests possible connections with clock neurons. These transgenes allow the considered neurons to be analyzed anatomically and functionally. The project will focus on neurons that might be downstream targets of the clock neurons that contribute to the evening peak of activity in light:dark cycles. We will use molecular-genetic tools to anatomically and functionally verify whether and how these putative downstream neurons are connected different types of evening clock neurons. The behavioral contribution of these neurons will be analyzed by manipulating their activity (hyperexcitation or silencing) in different environmental conditions. The experiments will involve Drosophila genetics, behavioral analysis (sleep-wake cycles), as well as anatomical and functional imaging (Ca2+) of the clock/target neurons.
Address: Paris-Saclay Institut of Neuroscience 1 av. de la terrasse, 91190 Gif-sur-Yvette
Phone: 33 1 69 82 34 36