Dynamique et Physiopathologie des Réseaux Neuronaux

Leader

Research center

11 place Marcelin Berthelot
75231 Paris
Alain Prochiantz

Institution

Collège de France
CNRS
Inserm
ED158
Université Pierre et Marie Curie

Laboratory

Centre Interdisciplinaire Recherche Biologie
Phone: 01 44 27 12 26
UMR 7241 - U1050
LabEx MemoLife

Mots clefs

Synaptic plasticity
dopamine
Learning and Memory
Parkinson’s disease
neuronal networks
basal ganglia
GABAergic interneurons
endocannabinoids
Available to host a PhD student

publications

Valtcheva S, Venance L. Astrocytes gate Hebbian synaptic plasticity in the striatum. Nat Commun. 2016 Dec 20;7:13845. doi: 10.1038/ncomms13845. 

Cui Y, Paille V, Xu H, Genet S, Delord B, Fino E, Berry H, Venance L. Endocannabinoids mediate bidirectional striatal spike-timing dependent plasticity. J Physiol. 2015 Apr 15. doi: 10.1113/JP270324.

Paille V#, Fino E#, Du K, Morera-Herreras T##, Perez S#, Hellgren Kotaleski J & Venance L# (2013) GABAergic circuits control spike-timing-dependent plasticity. J Neurosci. 33:9353-9363.

Nelson MJ, Bosch C#, Venance L# & Pouget P (2013) Microscale inhomogeneity of brain tissue distorts electrical signal propagation. J Neurosci. 33(7):2821-7.

Bosch C#, Mailly P, Degos B#, Deniau JM# & Venance L #(2012) Preservation of the hyperdirect pathway of basal ganglia in a rodent brain slice. Neuroscience 215, 31-41.

Evans RC, Morera-Herreras T#, Cui Y#, Du K, Sheehan T, Kotaleski JH, Venance L# & Blackwell KT (2012) The effects of NMDA subunit composition on calcium influx and spike timing-dependent plasticity in striatal medium spiny neurons. PLoS Comput Biol. 8, e1002493.

Puente N, Cui Y#, Lassalle O, Lafourcade M, Georges F, Venance L#*, Grandes P* & Manzoni OJ* (2011) Polymodal activation of the endocannabinoid system in the extended amygdala. Nature Neuroscience 14(12), 1542-7. *: co-senior authors Pandolfo, P, Silveirinha, V, dos Santos-Rodrigues, A, Venance, L#, Ledent, C, Takahashi, RN, Cunha, RA & Köfalvi, A 2011

Cannabinoids inhibit the synaptic uptake of adenosine and dopamine in the rat and mouse striatum?, Eur J Pharmacol. Vol.655(1-3), pp. 38-45.

Fields of research

Neurophysiology / systems neuroscience

Research Theme

Nos projets de recherche concernent le codage de la mémoire et l’apprentissage dans les ganglions de la base, un ensemble de noyaux sous-corticaux impliqués dans le contrôle adaptif du comportement. Les ganglions de la base, connectés en retour au cortex cérébral et au système limbique, participent a la sélection d’actions en relation avec la motivation et la prédiction de récompense.

La pathophysiologie des ganglions de la base conduit à des désordres moteurs et cognitifs majeurs (maladie de Parkinson, TOCs, syndrome de Tourette, addiction, …) pour lesquels il n’existe pas de traitements satisfaisants jusqu’à présent.
Nous étudions différents aspects des interactions synaptiques sous-tendant les propriétés dynamiques du réseau des ganglions de la base en conditions physiologiques et pathologiques. Nous utilisons une approche multidisciplinaire combinant l’électrophysiologie (multi-patch-clamp in vitro et enregistrements in vivo), la fast-cyclic voltammétrie, l’imagerie bi-photonique, la single-cell RT-PCR et l’immunohistochimie. La variété des expertises dans notre équipe ainsi que les collaborations que nous avons établies avec des mathématiciens, des biologistes moléculaires, des biochimistes et des cliniciens, nous permet d’analyser les fonctions physiologiques et pathologiques des ganglions de la base à différents niveaux de complexité du réseau neuronal. Les principaux projets sont:
1) Les propriétés dynamiques et la plasticité synaptique (STDP) dans les réseaux neuronaux des ganglions de la base et du cortex.

2) Les interactions neurone-glie : nuos analysons la contribution de la recapture de neurotransmetteurs par les astrocytes dans le traitement des informations cortico-striatales.

3) Les propriétés neuromodulatrices de la dopamine et des endocannabinoides.

Lab rotation

Role of striatal interneurons in goal directed behavior

Chercheur responsable: 

VENANCE Laurent

Dates: 

2 January 2018 - 29 June 2018

Date limite de candidature: 

29 June 2018

Period

~ Jan-March 2018

~ April-June 2018

Project

Basal ganglia are involved in goal-directed behavior and procedural learning. Striatum, the main input nucleus of basal ganglia, is composed of a majority of striatal projection neurons, but also of a variety of GABAergic interneurons which, and exert a strong feedforward inhibition. As we recently showed, GABAergic interneurons efficiently control synaptic hebbian plasticity (Paille et al., 2013, J Neurosci; Valtcheva et al., 2017, Neuropharmacol; Valtcheva and Venance, 2017, Nat Comm).

In this project we aim at optogenetically activating or inhibiting specifically each GABAergic subpopulation in dorsal striatum and observe the impact on a goal directed task and the associated procedural learning and recall. Neuronal activity in the behaving mice will be concomitantly examined with multi-channels recordings.

Techniques: in vivo optogenetics, behavioral tasks, in vivo multi-channel recordings, immunohistochemistry.

Contact

Collège de France - CIRB - 11, Place Marcelin Berthelot 75005 Paris - +33 1 44 27 12 26 - laurent.venance@college-de-france.fr

Superviseur: 

VENANCE Laurent & VANDECASTEELE Marie

Targeting GABAergic interneurons of the motor cortex as a therapeutic strategy in Parkinson's disease

Chercheur responsable: 

VENANCE Laurent

Dates: 

2 January 2018 - 29 June 2018

Date limite de candidature: 

29 June 2018

Period

~ Jan-March 2018

~ April-June 2018

Project

The target of our study is the inhibitory GABAergic neurons of the motor cortex. Indeed, recent evidence in human patients have questioned the classical model of Parkinson's disease, and indicate that cortical inhibition is reduced in PD. The potential beneficial effects of manipulating motor cortex inhibitory networks have never been directly assessed neither in humans nor in animal models of PD. Our results show that the high frequency stimulation of the subthalamic nucleus (HFS-STN), an efficient but costly therapeutic approach for Parkinson's disease which mechanisms remain largely elusive, involves the recruitment of cortical inhibitory networks. This project aims to evaluate the behavioral effect of the direct recruitment of cortical interneurons by optogenetics, as a potential alternative to HFS-STN or an adjunctive treatment.

Techniques: in vivo optogenetics, behavioral tasks, in vivo multi-channel recordings, immunohistochemistry.

Contact

Collège de France - CIRB - 11, Place Marcelin Berthelot 75005 Paris - +33 1 44 27 12 26 - laurent.venance@college-de-france.fr

Superviseur: 

VENANCE Laurent & DEGOS Bertrand

Dopaminergic control of cortical and thalamic interplay for striatal synaptic plasticity

Chercheur responsable: 

VENANCE Laurent

Dates: 

2 January 2018 - 29 June 2018

Date limite de candidature: 

29 June 2018

Period

~ Jan-March 2018

~ April-June 2018

Project

Basal ganglia are involved procedural learning and memory. Striatum, the main inputs nucleus of basal ganglia, acts as a coincidence detector of cortical and thalamic inputs. Dopamine tunes this striatal detection threshold. We have pioneered the field of spike-timing dependent plasticity, a synaptic Hebbian learning rule, at the level of basal ganglia (Fino et al., 2005, J Neurosci; Paillé et al., 2013, J Neurosci; Cui et al., 2016, eLife; Vlatcheva and Venance, 2017, Nat Comm). 

We are exploring the impact on striatal synaptic plasticity of cortical and thalamic activities timed with dopamine opto-stimulation or inhibition. This will be achieved in vitro and in vivo in physiological conditions and in a rodent model of Parkinson’s disease. This project aims at a better understanding how cortical and thalamic activity together with dopamine concurred to striatal synaptic plasticity and the engram of procedural learning.Techniques: electrophysiology (ex vivo patch-clamp), 2-photon imaging, optogenetics, multi-channel recordings.

Contact

Collège de France - CIRB - 11, Place Marcelin Berthelot 75005 Paris - +33 1 44 27 12 26 - laurent.venance@college-de-france.fr

Superviseur: 

VENANCE Laurent