De la stimulation sensorielle à l'hyperémie fonctionnelle

Leader

Research center

45 rue des Saints Pères
75006 Paris
Daniel Zytnicki

Institution

Inserm
CNRS
Université Paris Descartes
ED 157 GC2ID / ED158
Université Paris Descartes / Université Pierre et Marie Curie

Laboratory

Neurophysiology & New Microscopies Laboratory
Phone: (+33) 1 42 86 41 48
U1128
Idex Sorbonne Paris Cité

Mots clefs

Olfaction
Microscopy of the living brain
Available to host a PhD student

publications

Lyons DG, Parpaleix A, Roche M, Charpak S.Mapping oxygen concentration in the awake mouse brain.Elife. 2016 Feb 2;5. pii: e12024. doi: 10.7554/eLife.12024.

Ducros M, Houssen YG, Bradley J, de S, V, Charpak S (2013) Encoded multisite two-photon microscopy. Proc Natl Acad Sci U S A.110 13138-13143.

Parpaleix A, Houssen YG, Charpak S. (2013) Imaging local neuronal activity by monitoring Po2 transients in capillaries. Nature med,19:241-246.

Charpak S, Stefanovic B (2012) Shedding light on the BOLD fMRI response. Nat Methods 9:547-549. (News and Views)

Lecoq, J, Parpaleix, A, Roussakis, E, Ducros, M., Goulam, Y, Vinogradov, S, Charpak, S (2011) Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels. Nature Med. 17:893-898.

Najac M., De Saintjan D,Reguero L,Grandes P,Charpak S (2011) Mono- and polysynaptic feed-forward inputs to mitral cells from olfactory sensory neurons, J Neurosci. 31:8722-8729.

Fields of research

Neurophysiology / systems neuroscience

Research Theme

Our goal is to understand how a sensory stimulus such as an odor generates a population activity in a small cellular network, the olfactory bulb glomerulus. We investigate this question by using or developing optical tools that can be combined with electrophysiological recordings and allow the observation of neuron and astrocyte activities in vitro and in vivo. During the last 5 years, our research aimed in three directions:

1. The improvement of optical methods to image cellular activity, blood flow and brain metabolism

2. The study of synaptic interactions within the glomerular network

The analysis of neurovascular coupling, functional hyperaemia and energy consumption in glomeruli.

Etudiants ENP

Declan Gerard LYONS

Lab rotation

Multiscale analysis of odor processing in the olfactory bulb

Chercheur responsable: 

CHARPAK Serge

Dates: 

1 September 2016 - 31 December 2016

Date limite de candidature: 

1 September 2016

~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017

Project:

The olfactory bulb glomerulus is, anatomically, a well-defined neuronal network. However, little is known about the role of individual cell types during glomerular activation by odour. Consequently, the interpretation of glomerular activation imaging, whether with cellular approaches (two-photon microscopy to image single/multiple cells, blood flow of tissue oxygenation) or macroscopic approaches (fMRI, fUltrasounds) remains unclear. During the rotation, the project will be to use two-photon microscopy to investigate the role of a specific cell type in odour perception. 

Address: UFR Biomédicale des ST Pères, 45 rue des Sts Pères 75006 Paris 

Email: serge.charpak@parisdescartes.fr

Website

Superviseur: 

Serge CHARPAK