Codes de signaux intracellulaires pour le guidage axonal


Research center

17 rue Moreau
75012 Paris
José-Alain Sahel


Université Pierre et Marie Curie


Phone: (+33) 1 53 46 25 57
UMRS968 UMR7210 UM80
Labex Lifesenses

Mots clefs

neuronal connectivity
Brain development and plasticity
Available to host a PhD student


Averaimo S, Assali A, Ros O, Couvet S, Zagar Y, Genescu I, Rebsam A, Nicol X. A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors. Nat Commun. 2016 Oct 3.

Aït-Ali N, Fridlich R, Millet-Puel G, Clérin E, Delalande F, Jaillard C, Blond F, Perrocheau L, Reichman S, Byrne LC, Olivier-Bandini A, Bellalou J, Moyse E, Bouillaud F, Nicol X, Dalkara D, van Dorsselaer A, Sahel JA, Léveillard T. Rod-derived cone viability factor promotes cone survival by stimulating aerobic glycolysis. Cell. 2015 May 7.

Averaimo S, Nicol X. Intermingled cAMP, cGMP and calcium spatiotemporal dynamics in developing neuronal circuits. Front Cell Neurosci. 2014 Nov 13.

Griesi-Oliveira K, Acab A, Gupta AR, Sunaga DY, Chailangkarn T, Nicol X, Nunez Y, Walker MF, Murdoch JD, Sanders SJ, Fernandez TV, Ji W, Lifton RP, Vadasz E, Dietrich A, Pradhan D, Song H, Ming GL, Gu X, Haddad G, Marchetto MC, Spitzer N, Passos-Bueno MR, State MW, Muotri AR. Modeling non-syndromic autism and the impact of TRPC6 disruption in human neurons. Mol Psychiatry. 2015 Nov.

Nicol X, Gaspar P. Routes to cAMP: shaping neuronal connectivity with distinct adenylate cyclases. Eur J Neurosci. 2014 Jun.

Plazas PV, Nicol X, Spitzer NC. Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3. Proc Natl Acad Sci U S A. 2013 Jan 22.

Voyatzis S, Muzerelle A, Gaspar P, Nicol X. Modeling activity and target-dependent developmental cell death of mouse retinal ganglion cells ex vivo. PLoS One. 2012.

Nicol X, Hong KP, Spitzer NC. Spatial and temporal second messenger codes for growth cone turning. Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13776-81.

Hong KP, Spitzer NC, Nicol X. Improved molecular toolkit for cAMP studies in live cells. BMC Res Notes. 2011 Jul 20;4:241

Nicol X, Voyatzis S, Muzerelle A, Narboux-Nême N, Südhof TC, Miles R, Gaspar P. cAMP oscillations and retinal activity are permissive for ephrin signaling during the establishment of the retinotopic map. Nat Neurosci. 2007 Mar;10(3):340-7.

Nicol X, Muzerelle A, Rio JP, Métin C, Gaspar P. Requirement of adenylate cyclase 1 for the ephrin-A5-dependent retraction of exuberant retinal axons. J Neurosci. 2006 Jan 18;26(3):862-72.

Fields of research

Neurogenetics / neurodevelopment

Research Theme

The mature nervous system is an intricate network in which neurons are connected to specific partners. The choice of these partners is crucial for the correct behavior of the network (meaning the nervous system) and is determined at early stages of development. Abnormal development of neuronal connections is responsible for a large range of neuronal pathologies, some of them affecting vision. Research carried on by our team focuses on a better understanding of the development of sensory maps including the connection between the retina and the brain.

We aim to identify the codes of intracellular signals guiding retinal axons to the brain and shaping their arbors in their targets. We focus on signals required for axons to detect and interpret attractive and repulsive cues of axonal environment or involved in axonal and synaptic competition. Cyclic nucleotides and calcium are crucial for axons to integrate extracellular signals required for axon pathfinding and to modulate axonal and synaptic competition. They are involved in a wide range of other signaling pathways. We aim to decrypt the codes used by axons and synapses to identify such cellular messenger signals as specific regulators of neuronal connectivity.

We use a combination of anatomical, imaging and optogenetic techniques to investigate the role of second messengers in the development of sensory maps. FRET imaging enables following cellular messenger concentration in living cells. Other techniques including subcellular targeting of cAMP and calcium signaling blockers, and the use of light sensitive tools (optogenetics) make possible precise manipulations of these signals in time and space. Using these tools we are able to test the importance of local and temporal coding of cellular signals during the development of neuronal networks.

Project 1 : Spatiotemporal codes of second messengers for the development of neuronal connectivity. Second messengers modulate a wide range of cellular processes. We investigate the spatial and temporal features of cyclic nucleotide and calcium signals shaping neuronal connectivity to understand how second messengers achieve selectivity for their wide range of downstream pathways in developing axons.

Project 2 : Cooperation between neighboring axons to finely tune neuronal connectivity. Once axonal arbors are coarsely organized in their targets, complex and activity-dependent interactions between axons lead to the elimination of misplaced branches and synapses. This process requires interactions between neighboring axons. We aim to better understand these interactions between populations of axons.

Membres de l'équipe

Stefania Averaimo
Oriol Ros
Sandrine Couvet
Alice Louail
Solène Ribes