Interface physics / technology / robotics

Functional imaging and behavior in zebrafish

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

light-sheet microscopy
neuroimaging
zebrafish
sensory-motor integration
circuit inference

Labelisation ENP: 

2016

Centre de recherche / Institut: 

Laboratoire Jean Perrin

Code unité de recherche: 

UMR 8237

The overall objective of the group is to unravel principles of neural computations underlying sensory-motor integration in the vertebrate brain. We use the zebrafish larva as it currently constitutes the only vertebrate system amenable to whole-brain recording with cellular resolution. Using one- or two-photon light-sheet microscopy, we are able to monitor the long-term activity of the quasi-entirety of the 100,000 neurons that comprise the animal brain, as it performs basic sensory-motor tasks.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Raphaël Candelier
Sergei Kruglik
Sébastien Wolf
Alexis Dubreuil
Sophia Karpenko
Guillaume Le Goc
Établissements

Établissement de rattachement: 

UPMC

Établissements affiliés: 

CNRS

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED 564
Publications

publications: 

Sensorimotor Computation underlying phototaxis in zebrafish. Sébastien Wolf, Alexis M. Dubreuil, Tommaso Bertoni, Urs Lucas Böhm, Volker Bormuth, Raphaël Candelier, Sophia Karpenko, David G. C. Hildebrand, Isaac H. Bianco, Rémi Monasson, Georges Debrégeas. Nature Communication, in press

Whole-brain functional imaging with two-photon light-sheet microscopy, S Wolf, W. Supatto, G. Debrégeas, P. Mahou, S. G Kruglik,J-M. Sintes, E. Beaurepaire, R. Candelier Nature Methods 12, 379–380 (2015)

An Amplitude Modulation/Demodulation Scheme for Whisker-Based Texture Perception, Y. Boubenec, L. N. Claverie, D. E. Shulz,G. Debrégeas, J. Neurosci. 34(33): 10832-10843 (2014)

Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy. T. Panier,S. Romano, R. Olive, T. Pietri, G. sumbre, R. Candelier, G. Debrégeas, Frontiers in Neural Circuits 7, 0065 (2013)

The role of fingerprints in the coding of tactile information probed with a biomimetic sensor, J. Scheibert, S. Leurent, A. Prevost et G. Debrégeas, Science 323 1503-1506 (2009). Reviewed in Science news, Science Update (radio), Science signaling, Nature news, Nature Research highlights, Nature Methods,Analytical Chemistry, Atten. Percept & Psych.

Signatures of granular microstructure in dense shear flows. D. Mueth, G. Debregeas, G. Karczmar, P. Eng, S. Nagel & H. Jaeger,Nature 406, 385-388 (2000)

Dynamique et structure neuronale

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

imaging
in vitro
Cannabinoids
remodeling in vivo

Labelisation ENP: 

2015

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

Research in my team is focused on neuronal G-protein Coupled Receptors (GPCRs). These sensory proteins are important targets oftherapeutic and abused drugs, and are usually studied by pharmacology or electrophysiology, but we assume that their functioncannot be fully understood without considering their sub-cellular environment. We have indeed found unexpectedly close relationshipbetween neuropharmacology and neuronal cell biology.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Sophie Pezet
Diana Zala
Établissements

École doctorale: 

ED3C
Laboratory

Initiatives d'Excellence: 

LabEx MemoLife
Publications

publications: 

Roland BA#, Ricobaraza A#, Carrel D#, Jordan MB, Rico F, Simon A#, Humbert-Claude M#, Ferrier J#, McFadden HM#, Scheuring S, Lenkei Z#. Cannabinoid-induced actomyosin contractility shapes neuronal morphology and growth, eLife, 2014, Sep 15;3. doi:10.7554/eLife.03159

Osmanski B, Pezet S#, Ricobaraza A#, Lenkei Z#*, Tanter M*. fUltrasound Imaging of Intrinsic Connectivity in the Living Rat Brain with High Spatiotemporal Resolution, Nature Communications, 2014, 2014 5:5023. *: co-senior authors

Thibault K#, Lin WK, Rancillac A, Fan M, Snollaerts T, Sordoillet V, Hamon M, Smith GM, Lenkei Z#, Sophie P#. BDNF-Dependent Plasticity Induced by Peripheral Inflammation in the Primary Sensory and the Cingulate Cortex Triggers Cold Allodynia and Reveals a Major Role for Endogenous BDNF As a Tuner of the Affective Aspect of Pain. J. Neurosci, 29 October 2014, 34(44):14739-14751

Ladarre D#, Roland BA#, Biedzinski S, Ricobaraza A#, Lenkei Z#. Polarized cellular patterns of endocannabinoid production and detection shape cannabinoid signaling in neurons. Frontiers in Cellular Neuroscience, 2014 (in press)

Simon AC#, Loverdo C, Gaffuri AL#, Urbanski M, Ladarre D#, Carrel D, Rivals I, Leterrier C#, Benichou O, Dournaud P, Szabo B, Voituriez R, Lenkei Z#. Activation-dependent plasticity of polarized GPCR distribution on the neuronal surface. J Mol Cell Biol. 2013 Aug;5(4):250-65.

Cybernétique des microcircuits thalamiques et corticaux

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Cell-machine interface
thalamo-cortical
synaptic homeostasy
neuronal magnetic field

Labelisation ENP: 

2014

Centre de recherche / Institut: 

Unité de Neuroscience, Information et Complexité

Code unité de recherche: 

NeuroPSI

Our team is specialized in electrophysiology in vitro. One of our main technological achievements is the development of a neuron-machine interface based on dynamic-clamp that allows the reconstruction of “hybrid circuits”, made of living neurons synaptically interacting with computer models. Current research interests include the modulation of sensory information processing in the thalamus and cortex by top-down background synaptic activity (in intra-UNIC collaboration with Alain Destexhe and Yves Frégnac’s teams).

Leader

Leader: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Sud

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED 158
Laboratory

Nom: 

UNIC CNRS

Initiatives d'Excellence: 

Idex NeuroSaclay
Publications

publications: 

See publications in Chercheurs/T. BAL 

Gomes, J.-M., bedard, C., Valtcheva, S., Nelson, M., Khokhlova, V., Pouget, P., Venance, L., Bal, T., & Destexhe, A. (2016) Intracellular Impedance Measurements Reveal Non-ohmic Properties of the Extracellular Medium around Neurons. Biophysj, 110, 234–246.

Ouanounou, G., Baux, G., & Bal, T. (2016) A novel synaptic plasticity rule explains homeostasis of neuromuscular transmission.eLife, 5 : e12190

Behuret, S., Deleuze, C., & Bal, T. (2015). Corticothalamic Synaptic Noise as a Mechanism for Selective Attention in Thalamic Neurons. Front Neural Circuits, 9, 11633

Casale, A.E., Foust, A.J., Bal, T., & McCormick, D.A. (2015). Cortical Interneuron Subtypes Vary in Their Axonal Action Potential Properties. Journal of Neuroscience, 35, 15555–15567

Transmission inhibitrice dans le cerveau

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Inhibitory transmission
Multiphoton imaging
Glycine
Vesicular loading
Optogenetics
Cerebellum

Labelisation ENP: 

2012

Centre de recherche / Institut: 

ENS Ecole Normale Supérieure

Code unité de recherche: 

U1024 - UMR8197

. La transmission synaptique inhibitrice joue un rôle central dans le fonctionnement du cerveau. Preuve en est que le blocage de l’inhibition engendre des situations pathologiques comme l’épilepsie et l’hyperekplexie, une affection rare caractérisée par des sursauts exagérés en réponse à des stimuli auditifs, visuels ou tactiles. A l’inverse, une potentialisation de l’inhibition provoque des états modifiés du cerveau comme ceux résultant de l’action de psychotropes, anxiolytiques, somnifères ou encore analgésiques.

Leader

Leader: 

Personnel

Membres de l'équipe: 

MARCAGGI Païkan
Établissements

Établissement de rattachement: 

Ecole Normale Supérieure

Établissements affiliés: 

Inserm
CNRS

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Institut de Biologie de l'ENS IBENS

Initiatives d'Excellence: 

Labex Memolife, IDEX PSL*, NIH Brain Initiative Grant
Publications

publications: 



Zampini V, Liu JK, Diana MA, Maldonado PP, Brunel N, Dieudonné S. (2016) Mechanisms and functional roles of glutamatergic synapse diversity in a cerebellar circuit. Elife. 2016 Sep 19;5. pii: e15872. doi: 10.7554/eLife.15872.

Akemann W, Léger JF, Ventalon C, Mathieu B, Dieudonné S, Bourdieu L. (2015) Fast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy. Opt Express. 23: 28191-205.

Giber K, Diana MA*, M Plattner V*, Dugué GP, Bokor H, Rousseau CV, Maglóczky Z, Havas L, Hangya B, Wildner H, Zeilhofer HU, Dieudonné S§, Acsády L. (2015) A subcortical inhibitory signal for behavioral arrest in the thalamus. Nat Neurosci. 18:562-8.

Ankri L, Husson Z*, Pietrajtis K, Proville R, Léna C, Yarom Y, Dieudonné S§, Yoe Uusisaari M. (2015) A novel inhibitory nucleo-cortical circuit controls cerebellar Golgi cell activity. Elife. 4. doi: 10.7554/eLife.06262.

Otsu Y, Marcaggi P, Feltz A, Isope P, Kollo M, Nusser Z, Mathieu B, Kano M, Tsujita M, Sakimura K, Dieudonné S§. (2014) Activity-dependent gating of calcium spikes by A-type K+ channels controls climbing fiber signaling in Purkinje cell dendrites. Neuron. 84 : 137-51.

Dynamique corticale et mécanismes de codage

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Neurosciences intégratives Modalité sensorielle tactile Cortex à tonneaux Microscopie de fluorescence à deux photons Optique adaptative

Labelisation ENP: 

2012

Centre de recherche / Institut: 

ENS Ecole Normale Supérieure

Code unité de recherche: 

U1024 UMR8197

Nous étudions la représentation de l’information sensorielle dans une colonne corticale en enregistrant optiquement l’activité de centaines de neurones. Nous nous intéressons en particulier à la représentation de l’information tactile collectée par les vibrisses des rongeurs et représentée dans le cortex somato-sensoriel primaire à tonneaux.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Wang Jinyu
Paresys Gérard
Léger Jean François
Ventalon Cathie
Établissements

Établissement de rattachement: 

Ecole Normale Supérieure

Établissements affiliés: 

Inserm
CNRS

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Laboratory

Nom: 

Institut de Biologie de l'ENS IBENS

Initiatives d'Excellence: 

Labex Memolife, Programme France Bio-Imaging, NIH Brain Initiative 2017
Publications

publications: 

L. Estebanez, J. Bertherat, D.E. Shulz, L. Bourdieu, J.-F. Léger, High order statistics of naturalistic stimuli are orderly mapped in the primary somatosensory cortex, (2016) Nature Comm. 7:13528.

J. Wang, J.-F. Leger, J. Binding, C. Boccara, S. Gigan and L. Bourdieu, Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer, Biomed. Opt. Exp. (2012) 3(10) 2510-25.

V. Szabo*, C. Ventalon*, V. De Sars, J. Bradley, and V. Emiliani, “Spatially selective photoactivation with computer generated holography and functional fluorescence imaging in freely behaving mice with a fiberscope”, Neuron, 84 (6), 1157-1169. (2014) * Equal contribution.

S. Schott, J. Bertolotti, J.F. Léger, L. Bourdieu and S. Gigan, Characterization of the angular memory effect of scattered light in biological tissues. Opt. Exp. 23(10) (2015) 13505-16, arXiv:1502.00270.

W. Akemann, J.-F. Léger, C. Ventalon, B. Mathieu, S. Dieudonné and L. Bourdieu, Fast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy, Opt. Express 23(22), 28191-28205 (2015).

 

Synapses gabaergiques du cervelet

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

Patch clamp
imagerie cellulaire
electrophysiologie

Labelisation ENP: 

2007

Centre de recherche / Institut: 

INCC - UMR 8002 - Paris Descartes University

Code unité de recherche: 

UMR 8118

L’équipe pratique une approche pluridisciplinaire incorporant des techniques physiques (optique), mathématiques (statistiques, modélisation) et chimiques (synthèse de composés). Elle développe des méthodes nouvelles pour étudier les mécanismes de communication cellulaire dans le tissu cérébral.

Leader

Leader: 

Personnel

Membres de l'équipe: 

David Ogden
Isabel Llano
Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Descartes

Université: 

Université Paris Descartes / Université Paris Diderot
Laboratory

Nom: 

Laboratoire de physiologie cérébrale
Publications

publications: 

Miki T, Malagon G, Pulido C, Llano I, Neher E, Marty A. Actin- and Myosin-Dependent Vesicle Loading of Presynaptic Docking Sites Prior to Exocytosis. Neuron. 2016 Aug 17;91(4):808-23. doi: 10.1016/j.neuron.2016.07.033.

Pulido C, Trigo FF, Llano I, Marty A. Vesicular release statistics and unitary postsynaptic current at single GABAergic synapses. Neuron. 2015 Jan 7;85(1):159-72. doi: 10.1016/j.neuron.2014.12.006. Epub 2014 Dec 24.

Alcami P, Marty A. Estimating functional connectivity in an electrically coupled interneuron network. Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):E4798-807. doi: 10.1073/pnas.1310983110. Epub 2013 Nov 18.

Lamotte d'Incamps B, Krejci E, Ascher P (2012) Mechanisms shaping the slow nicotinic synaptic current at the motoneuron-renshaw cell synapse. J. Neurosci 32:8413-8423

Mejia-Gervacio, S. V. (2012). Muscarinic control of AMPA receptor responsiveness in mouse spinal cord motoneurons. J. Physiol. in press.

Palma-Cerda F, Auger C, Crawford DJ, Hodgson ACC, Reynolds SJ, Cowell JK, Swift KAD, Cais O, Vyklicky L, Corrie JET, Ogden D (2012) New caged neurotransmitter analogs selective for glutamate receptor sub-types based on methoxynitroindoline and nitrophenylethoxycarbonyl caging groups. Neuropharmacology 63:624-634

Pessac B, Nimmagadda VK, Makar T, Fishman PS, Bever CT, Trisler D (2012) Adult hematopoietic progenitors are multipotent in chimeric mice. C. R. Biol 335:454-462

Rossi B, Ogden D, Llano I, Tan YP, Marty A, Collin T (2012) Current and Calcium Responses to Local Activation of Axonal NMDA  Receptors in Developing Cerebellar Molecular Layer Interneurons. PLoS One 7 7(6): e39983



De la stimulation sensorielle à l'hyperémie fonctionnelle

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Olfaction
Microscopy of the living brain

Labelisation ENP: 

2007

Centre de recherche / Institut: 

Institut de la Vision

Code unité de recherche: 

U1128

Our goal is to understand how a sensory stimulus such as an odor generates a population activity in a small cellular network, the olfactory bulb glomerulus. We investigate this question by using or developing optical tools that can be combined with electrophysiological recordings and allow the observation of neuron and astrocyte activities in vitro and in vivo. During the last 5 years, our research aimed in three directions:

1. The improvement of optical methods to image cellular activity, blood flow and brain metabolism

Leader

Leader: 

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

CNRS
Université Paris Descartes

Université: 

Université Paris Descartes / Université Pierre et Marie Curie

École doctorale: 

ED 157 GC2ID / ED158
Laboratory

Nom: 

Neurophysiology & New Microscopies Laboratory

Initiatives d'Excellence: 

Idex Sorbonne Paris Cité
Publications

publications: 

Lyons DG, Parpaleix A, Roche M, Charpak S.Mapping oxygen concentration in the awake mouse brain.Elife. 2016 Feb 2;5. pii: e12024. doi: 10.7554/eLife.12024.

Ducros M, Houssen YG, Bradley J, de S, V, Charpak S (2013) Encoded multisite two-photon microscopy. Proc Natl Acad Sci U S A.110 13138-13143.

Parpaleix A, Houssen YG, Charpak S. (2013) Imaging local neuronal activity by monitoring Po2 transients in capillaries. Nature med,19:241-246.

Charpak S, Stefanovic B (2012) Shedding light on the BOLD fMRI response. Nat Methods 9:547-549. (News and Views)

Lecoq, J, Parpaleix, A, Roussakis, E, Ducros, M., Goulam, Y, Vinogradov, S, Charpak, S (2011) Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels. Nature Med. 17:893-898.

Najac M., De Saintjan D,Reguero L,Grandes P,Charpak S (2011) Mono- and polysynaptic feed-forward inputs to mitral cells from olfactory sensory neurons, J Neurosci. 31:8722-8729.

Dynamique oscillante et stochastique des réseaux thalamocorticaux

Domaine de recherche principal: 

Computational neurosciences / neural theory

Labelisation ENP: 

2007

Centre de recherche / Institut: 

Unité de Neuroscience, Information et Complexité

Code unité de recherche: 

UPR 3293

Propriétés intégratives des neurones du neocortex dans les états de haute conductance.

Leader

Leader: 

Personnel

Etudiants ENP: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Sud

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158 - 3C
Laboratory

Initiatives d'Excellence: 

IDEX NeuroSaclay
Publications

publications: 

Dehghani, N., Peyrache, A., Telenczuk, B., Le Van Quyen, M., Halgren, E., Cash, S.S., Hatsopoulos, N.G. and Destexhe, A. Dynamic balance of excitation and inhibition in human and monkey neocortex.  Nature Scientific Reports 6: 23176, 2016.  DOI: 10.1038/srep23176

Zerlaut, Y. and Destexhe, A. Enhanced responsiveness and low-level awareness in stochastic network states.  Neuron 7: 1002-1009, 2017. DOI: 10.1016/j.neuron.2017.04.001

Telenczuk, B., Dehghani, N., Le Van Quyen, M., Cash, S., Halgren, E., Hatsopoulos, N.G. and Destexhe, A. Local field potentials primarily reflect inhibitory neuron activity in human and monkey cortex.  Nature Scientific Reports 7: 40211, 2017.  DOI: 10.1038/srep40211

Barbieri, F., Trauchessec, V.. Caruso, L., Trejo Rosillo, J., Telenczuk, B., Paul, E., Bal, T., Destexhe, A., Fermon, C., Pannetier-Lecoeur, M. and Ouanounou, G. Local recording of biological magnetic fields using Giant Magneto Resistance-based micro-probes.  Nature Scientific Reports 6: 39330, 2016.  DOI: 10.1038/srep39330

Le Van Quyen, M., Muller, L., Telenczuk, B., Cash, S.S., Halgren, E., Hatsopoulos, N.G., Dehghani, N. and Destexhe, A. High-frequency oscillations in human and monkey neocortex during the wake-sleep cycle.  Proc. Natl. Acad. Sci. USA 113: 9363-9368, 2016.  DOI: 10.1073/pnas.1523583113

Cognisciences: Intégration et plasticité synaptique dans le cortex visuel

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Vision
electrophysiology
imaging
Multiscale
Modeling

Labelisation ENP: 

2007

Centre de recherche / Institut: 

Unité de Neuroscience, Information et Complexité

Code unité de recherche: 

UPR 3293

An issue central to the theme of complexity in the dynamics and function of biological networks concerns the inferences that can be made from multiscale measurements ranging from microscopic to macroscopic levels of integration. Our research is based on interdisciplinary approaches ranging from electrophysiology (intracellular sharp and patch recordings, in vivo), network imaging (voltage sensitive dye), psychophysical measurements to functional databasing and phenomenological and computational modeling.

Personnel

Etudiants ENP: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Sud

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158 - 3C
Laboratory

Initiatives d'Excellence: 

Idex i-Code, Idex NeuroSaclay
Publications

publications: 

Gerard-Mercier F, Carelli PV, Pananceau M, Troncoso XG, Frégnac Y. Synaptic Correlates of Low-Level Perception in V1. J Neurosci. 2016 Apr 6;36(14):3925-42. doi: 10.1523/JNEUROSCI.4492-15.2016.

Fournier J, Monier C, Levy M, Marre O, Sári K, Kisvárday ZF, Frégnac Y. Hidden complexity of synaptic receptive fields in cat V1. J Neurosci. 2014 Apr 16;34(16):5515-28. doi: 10.1523/JNEUROSCI.0474-13.2014.

Arduin P.J. , Ego-Stengel V. , Frégnac Y. and Shulz D.E. (2013) Master neurons induced by operant conditioning in rat motor cortex during a brain-machine interface task. The Journal of Neuroscience. 33(19): 8308-20.

Levy M. , Fournier J. and Frégnac Y. (2013) The role of delayed suppression in slow and fast contrast adaptation in v1 simple cells. The Journal of Neuroscience. 33(15):6388-400.

El Boustani S. , Yger P. , Destexhe A. and Frégnac Y. (2012) A. Stable learning in stochastic network states. J. Neurosci. 32: 194-214.

Frégnac Y. (2012) Reading out the synaptic echoes of low level perception in V1. Lecture Notes in Computer Science. 7583: 486-495.

Chavane F. , Sharon D. , Jancke D. , Marre O. , Frégnac Y. and Grinvald A. (2011) Lateral spread of orientation selectivity in V1 is controlled by intracortical cooperativity. Frontiers in System Neuroscience, 5:4. 1-26.