UMRS 894

Dynamique et structure neuronale

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

imaging
in vitro
Cannabinoids
remodeling in vivo

Labelisation ENP: 

2015

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

Research in my team is focused on neuronal G-protein Coupled Receptors (GPCRs). These sensory proteins are important targets oftherapeutic and abused drugs, and are usually studied by pharmacology or electrophysiology, but we assume that their functioncannot be fully understood without considering their sub-cellular environment. We have indeed found unexpectedly close relationshipbetween neuropharmacology and neuronal cell biology.

Leader

Leader: 

Personnel

Membres de l'équipe: 

Sophie Pezet
Diana Zala
Établissements

École doctorale: 

ED3C
Laboratory

Initiatives d'Excellence: 

LabEx MemoLife
Publications

publications: 

Roland BA#, Ricobaraza A#, Carrel D#, Jordan MB, Rico F, Simon A#, Humbert-Claude M#, Ferrier J#, McFadden HM#, Scheuring S, Lenkei Z#. Cannabinoid-induced actomyosin contractility shapes neuronal morphology and growth, eLife, 2014, Sep 15;3. doi:10.7554/eLife.03159

Osmanski B, Pezet S#, Ricobaraza A#, Lenkei Z#*, Tanter M*. fUltrasound Imaging of Intrinsic Connectivity in the Living Rat Brain with High Spatiotemporal Resolution, Nature Communications, 2014, 2014 5:5023. *: co-senior authors

Thibault K#, Lin WK, Rancillac A, Fan M, Snollaerts T, Sordoillet V, Hamon M, Smith GM, Lenkei Z#, Sophie P#. BDNF-Dependent Plasticity Induced by Peripheral Inflammation in the Primary Sensory and the Cingulate Cortex Triggers Cold Allodynia and Reveals a Major Role for Endogenous BDNF As a Tuner of the Affective Aspect of Pain. J. Neurosci, 29 October 2014, 34(44):14739-14751

Ladarre D#, Roland BA#, Biedzinski S, Ricobaraza A#, Lenkei Z#. Polarized cellular patterns of endocannabinoid production and detection shape cannabinoid signaling in neurons. Frontiers in Cellular Neuroscience, 2014 (in press)

Simon AC#, Loverdo C, Gaffuri AL#, Urbanski M, Ladarre D#, Carrel D, Rivals I, Leterrier C#, Benichou O, Dournaud P, Szabo B, Voituriez R, Lenkei Z#. Activation-dependent plasticity of polarized GPCR distribution on the neuronal surface. J Mol Cell Biol. 2013 Aug;5(4):250-65.

Physiopathologie des troubles de l'humeur : dépression et addiction

Domaine de recherche principal: 

Neurological and psychiatric diseases

Mots clefs: 

plasticity
Stress
Depression
serotonine/
alcohol

Labelisation ENP: 

2014

Code unité de recherche: 

UMRS 894

The goal of the research team is to analyze the roles played by environmental and genetic factors, as well as the interactions between these components, in the etiology and treatment of psychiatric disorders such as depression, anxiety and addiction. The main objective of the team is to identify cellular and molecular mechanisms underlying these pathologies which are associated with major dysfunctions of the monoamine and HPA systems and characterized by cellular plasticity alterations of different brain areas and in particular the hippocampus.

Établissements

Établissement de rattachement: 

Inserm
Publications

publications: 

Stragier E, Massart R, Salery M, Hamon M, Geny D, Martin V, Boulle F, Lanfumey L. Ethanol-induced epigenetic regulations at the Bdnf gene in C57BL/6J mice. Neuroscience. 2014 Jun 20;271:56-63. doi: 10.1016/j.neuroscience.2014.04.027. Epub 2014 Apr 26.

Doucet EL, Lanfumey L, et al. (2013) Sustained impairment of alpha2A-adrenergic autoreceptor signaling mediates neurochemical and behavioral sensitization to amphetamine. Biol Psychiatry. 74:90-98

Garcia Garcia Al, Lanfumey L, et al. (2013) Regulation of serotonin (5-HT) function by a VGLUT1 dependent glutamate pathway. Neuropharmacology. 70:190-199

Lanfumey L, et al (2012) Biological rhythms and melatonin in mood disorders and their treatments Pharmacol Ther. 138:176-184 Review

Lanteri C, ?, Lanfumey L, et al.. (2013) Repeated exposure to MDMA triggers long-term plasticity of noradrenergic and serotonergic neurons Mol Psychiatry. 2013 Aug 20.

Martin CBP, .. Lanfumey L, .. et al. (2012) RNA splicing and editing modulation of 5-HT2C receptor function: relevance to anxiety and aggression in VGV mice. Mol Psychiatry. 18:656-665

Molet J,  Lanfumey L. Juvenile ethanol exposure increases rewarding properties of cocaine and morphine in adult DBA/2J mice. Eur Neuropsychopharmacol. 2013 Apr 22.

INTERACTIONS ENTRE NEURONES ET OLIGODENDROGLIES DANS LA MYÉLINISATION ET LA RÉPARATION DE LA MYÉLINE

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Patch clamp
Optogenetics

Labelisation ENP: 

2014

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

Oligodendrocyte precursor cells expressing the chondroitin sulfate proteoglycan NG2, also called NG2 cells, have the ability to proliferate in the postnatal brain to generate oligodendrocytes in grey and white matters. NG2 cells play a critical role in myelination during postnatal brain development, but a pool of these progenitors is maintained in the adult and recruited to lesions in demyelinating diseases. Recent discoveries have demonstrated that NG2 cells are contacted by functional glutamatergic and GABAergic synapses from neurons in grey and white matters.

Leader

Leader: 

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

Université Paris Descartes

École doctorale: 

GC2IDED BioSPC ED572
Publications

publications: 

Balia M*, Vélez-Fort M*, Passlick S, Schäfer C, Audinat E, Steinhäuser C, Seifert G, Angulo MC (2015) Postnatal down-regulation of the GABAA receptor 2 subunit in neocortical NG2 cells accompanies synaptic-to-extrasynaptic switch in GABAergic transmission mode. Cereb Cortex, 25(4):1114-23

Balia M, Benamer N, Angulo MC (2017) A specific GABAergic synapse onto oligodendrocyte precursors does not regulate cortical oligodendrogenesis. Glia. 65(11):1821-1832.

Ledonne F, Orduz D, Mercier J, Vigier L, Grove EA, Tissir F, Angulo MC, Pierani A*, Coppola E*. (2016) Targeted inactivation of Bax reveals subtype-specific mechanism of Cajal-Retzius neuron death in the postnatal cerebral cortex. Cell Rep. 17(12) 3133

Wake H*, Ortiz FC*, Woo DH, Lee P, Angulo MC, Fields D (2015) Non-synaptic junctions on myelinating glia promote preferential myelination of electrically-active axons. Nat Commun 6:7844

Orduz D*, Maldonado PP*, Balia M, Vélez-Fort M, de Sars V, Yanagawa Y, Emiliani V, Angulo MC (2015) Interneurons and oligodendrocyte progenitors form a structured synaptic network in the developing neocortex. eLife 4:e06953

 

Plasticité synaptique et réseaux neuronaux

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Hippocampe, mémoire, interneurones, pasticité synaptique, émotions

Labelisation ENP: 

2012

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

Notre équipe s’intéresse aux mécanismes impliqués dans la formation de la mémoire. L’hippocampe est une structure importante pour l’apprentissage et la formation de nouvelles mémoires. Le réseau d’interneurones inhibiteurs joue un rôle important dans le contrôle de l’activité hippocampique. Notre recherche est focalisée sur la région CA2, une région qui contient une très forte densité d’interneurones et qui est spécifiquement connectée par des afférences extra-hippocampiques venant notamment du cortex ou de l’hypothalamus.

Leader

Leader: 

Co leader: 

Personnel

Etudiants ENP: 

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

Sainte Anne

Université: 

Université Paris Descartes
Publications

publications: 

Piskorowski RA, Nasrallah K, Diamantopoulou A, Mukai J, Hassan SI, Siegelbaum SA, Gogos JA, Chevaleyre V.Age-Dependent Specific Changes in Area CA2 of the Hippocampus and Social Memory Deficit in a Mouse Model of the 22q11.2 Deletion Syndrome.Neuron. 2016 Jan 6;89(1):163-76. doi: 10.1016/j.neuron.2015.11.036.

Piskorowski R.A. and Chevaleyre V. (2012) Synaptic integration by different dendritic compartments of hippocampal CA1 and CA2 pyramidal neurons. Cellular and Molecular Life Science 69(1); 75-88.

Pavlopoulos E; Trifilieff P; Chevaleyre V; Zairis S; Fioriti L; Malleret G; Kandel E.R. (2011) Non-Proteolytic Ubiquitination by Neuralized1 Leads to Activation of CPEB3: A Novel Function of the Ubiquitin System in Synaptic Plasticity and Memory Storage. Cell 147(6); 1369-83.

Piskorowski R, Santoro B, Siegelbaum SA. (2011) TRIP8b splice forms act in concert to regulate the localization and expression of HCN1 channels in CA1 pyramidal neurons. Neuron. 70(3):495-509

Chevaleyre V. and Siegelbaum S.A. (2010) Strong CA2 pyramidal neuron synapses define a powerful disynaptic cortico-hippocampal loop. Neuron 66(4):560-72

Chevaleyre V., Heifets B.D., Kaeser P., Südhof T.C., Castillo P.E. (2007) Endocannabinoid-mediated long-term plasticity requires cAMP/PKA signalling and RIM1. Neuron.  54 (5): 801-12.

Physiopathologie des Maladies Psychiatriques

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Mots clefs: 

Psychose
Depression
addiction
anxiété
trouble bipolaire

Labelisation ENP: 

2008

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

L’objectif général du laboratoire de « Physiopathologie des Maladies Psychiatriques » est d’étudier les mécanismes impliqués dans le développement de maladies psychiatriques selon des méthodologies complémentaires, dans des recherches cliniques et grâce à des modélisations chez l’animal. Notre groupe de recherche clinique et fondamentale examine la prévalence de certains facteurs (exposition au stress, remodelage pubertaire) dans l’évolution de la vulnérabilité à la maladie (psychoses, dépression , autres), en interaction avec le « background » génétique et l'influence des thérapeutiques.

Leader

Leader: 

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

Université Paris Descartes

École doctorale: 

ED BioSPC ED 158 ED 563
Laboratory

Initiatives d'Excellence: 

Idex Sorbonne Paris Cité
Publications

publications: 

Renard J, Vitalis T, Rame M, Krebs M-O, Lenkei Z, Le Pen G, Jay TM. Chronic cannabinoid exposure during adolescence leads to long-term structural and functional changes in the prefrontal cortex. Eur Neuropsychopharmacol, 2015, in press.

Alexandre Ch, Chaumette B. Martinez G, Christia L, Dupont JM, Kebir O, Gaillard R, Amado I, Krebs MO. Paradoxical improvement of schizoprenic symptoms by a dopaminergic agonist : an example of personalized psychiatry in a CNV carrying patient. Biological Psychiatry. 2015 in press

Chan MK, Krebs MO, Cox D, Guest PC, Yolken RH, Rahmoune H, Rothermundt M, Steiner J, Leweke FM, van Beveren NJ, Niebuhr DW, Weber NS, Cowan DN, Suarez-Pinilla P, Crespo-Facorro B, Mam-Lam-Fook C, Bourgin J, Wenstrup RJ, Kaldate RR, Cooper JD, Bahn S. Development of a blood-based molecular biomarker test for identification of schizophrenia before disease onset. Transl Psychiatry. 2015 Jul 14;5:e601.

Chaumette B, Kebir O, Mam-Lam-Fook C, Morvan Y, Bourgin J, Godsil BP, Plaze M, Gaillard R, Jay TM, Krebs MO. Salivary cortisol in early psychosis: new findings and meta-analysis. Psychoneuroendocrinology, 2015 Oct 16;63:262-270.

Chaumette B, Kebir O, Mam-Lam-Fook C, Bourgin J, Godsil BP, Gaillard R, Jay TM, Krebs MO. Stress et Transition Psychotique : revue de la littérature - Encéphale in press

Trafic membranaire dans le cerveau normal et pathologique

Domaine de recherche principal: 

Neurogenetics / neurodevelopment

Mots clefs: 

mouse genetics
Exocytosis
SNARE
Biophysics
Microscopy

Labelisation ENP: 

2007

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

L’objectif de l’équipe est de comprendre les mécanismes moléculaires et cellulaires impliquant le trafic membranaire et l’adhérence cellule-cellule dans la morphogenèse neuronale et épithéliale. Nous nous intéressons particulièrement aux voies d’exocytose sensible et insensible à la neurotoxine tétanique dans la croissance axonale et la migration des cellules épithéliales. Nous étudions la fonction des protéines SNAREs vésiculaires cellubrévine, synaptobrévine et TI-VAMP, impliquées dans des voies d’exocytose et de deux molécules d’adhérence cellule-cellule : la vézatine et L1-CAM.

Leader

Leader: 

Personnel

Etudiants ENP: 

Membres de l'équipe: 

Thierry Galli, DR1 INSERM, DU
Christian Vannier, DR2 INSERM
Lydia Danglot, CR1 INSERM
David Tareste, CR1 INSERM
Sébastien Nola, IR2 INSERM
Agathe Verraes, TCS CNRS
Jose Wojnacki, Postdoc
Guan Wang, doc.
Ahmed Zahraoui, DR CNRS
Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

Sainte Anne

Université: 

Université Paris Descartes
Laboratory

Initiatives d'Excellence: 

Labex ‘WhoAmI ?’ Investissement d’Avenir, Plateforme Distribuée ‘FranceBioImaging-FBI’
Publications

publications: 

Petkovic M, Jemaiel A, Daste F, Specht CG, Izeddin I, Vorkel D, Verbavatz JM, Darzacq X, Triller A, Pfenninger KH, Tareste D, Jackson CL, Galli T. The SNARE Sec22b has a non-fusogenic function in plasma membrane expansion. Nat Cell Biol. 2014 May;16(5):434-44. doi: 10.1038/ncb2937. Epub 2014 Apr 6.

Larghi P, Williamson DJ, Carpier JM, Dogniaux S, Chemin K, Bohineust A, Danglot L, Gaus K, Galli T§, Hivroz C§. (2013) VAMP7 controls T cell activation by regulating the recruitment and phosphorylation of vesicular Lat at TCR-activation sites. Nat Immunol. doi:10.1038/ni.2609. § co-senior authors. (F1000)

Burgo A., Proux-Gillardeaux, V., Sotirakis, E., Bun, P., Casano,A., Verraes, A., Liem, R., Formstecher, E., Coppey, M. Galli, T. (2012). A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery. Developmental Cell,23:166?180.

Zylbersztejn K, Petkovic M, Burgo A, Deck M, Garel S, Marcos S, Bloch-Gallego E, Nothias F, Serini G, Bagnard D, Binz T, Galli T. (2012). The vesicular SNARE Synaptobrevin is required for Semaphorin 3A axonal repulsion. J Cell Biol 196:37-46. (F1000)

Danglot L*, Zylbersztejn K*, Petkovic M*, Meziane H, Combe R, Champy Mf, Birling Mc, Pavlovic G, Bizot Jc, Trovero F, Della Ragione F, Proux-Gillardeaux V, Sorg T, D?esposito M, Galli T. (2012). Absence of TI-VAMP/Vamp7 leads to increased anxiety in mice. J Neurosci 32:1962-1968 (F1000)

Danglot L, Chaineau M, Dahan M, Gendron M-C, Boggetto N, Perez F, and Galli T. (2010). Role of TI-VAMP and CD82 in EGF receptor cell surface dynamics and signaling. J Cell Sci 123:723-35.

Génétique et développement du cortex cérébral

Domaine de recherche principal: 

Neurogenetics / neurodevelopment

Labelisation ENP: 

2012

Centre de recherche / Institut: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Code unité de recherche: 

UMRS 894

Le cortex cérébral est le siège principal des fonctions cognitives. Les capacités intellectuelles de l'Homme constituent une caractéristique majeure qui le distingue au sein du phylum des vertébrés, incluant les autres mammifères.

Nos projets visent à  comprendre les mécanismes moléculaires qui contrôlent la diversité des cellules et leur positionnement dans le cortex cérébral au cours du développement.

Leader

Leader: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Diderot
Publications

publications: 

Ledonne F., Orduz D., Mercier J., Vigier L., Grove E.A., Tissir F., Angulo M.C., Pierani A. and Coppola E. Targeted inactivation of Bax reveals subtype-specific mechanism of Cajal-Retzius neuron death in the postnatal cerebral cortex.  Cell Reports (2016), 17, 3133–3141.

Freret-Hodara B., Cui Y., Griveau A., Vigier L., Arai Y., Touboul J. and Pierani A. Enhanced abventricular proliferation compensates cell death in the embryonic cerebral cortex. Cerebral Cortex (2016) Sept 12; doi: 10.1093/cercor/bhw264.

Karaz S.#, Courgeon M. #, Lepetit H., Bruno E., Pannone R., Tarallo A., Thouzé F., Kerner P., Vervoort M., Causeret F., Pierani A. and D'Onofrio G. Neuronal fate specification by the Dbx1 transcription factor is linked to the evolutionary acquisition of a novel functional domain. Evodevo (2016) Aug 12; doi: 10.1186/s13227-016-0055-5. eCollection 2016.

Barber, M., Arai, Y., Morishita, Y., Vigier, L., Causeret, F., Borello, U., Ledonne, F., Coppola, E., Contremoulins, V., Pfrieger, F.W., Tissir, F., Govindan, S., Jabaudon, D., Proux-Gillardeaux, V., Galli, T. and Pierani, A. Migration speed of Cajal-Retzius cells modulated by vesicular trafficking controls the size of higher-order cortical areas. Current Biol. (2015), 25, 2466-2478. Epub 2015 Sep 17. Research Highlight in Nature Reviews Neuroscience (2015), 16, 644-645

Causeret, F., Sumia, I. and Pierani A. Kremen1 and Dickkopf1 control cell survival in a Wnt-independent manner. Cell Death and Differentiation (2015), Jul 24. doi: 10.1038/cdd.2015.100.

Underligned publications were signed as corresponding author. # : equal contribution

 

Vulnérabilité aux troubles psychiatriques et addictifs

Labelisation ENP: 

2012

Code unité de recherche: 

UMRS 894

Le laboratoire axe son travail sur la recherche de facteurs prédictifs et/ou de vulnérabilité aux comportements addictifs.

Établissements

Établissement de rattachement: 

Inserm

Établissements affiliés: 

Université Paris Descartes

Université: 

Université Pierre et Marie Curie

École doctorale: 

ED158
Publications

publications: 

Lepagnol-Bestel AM et al. Deficiency of AUTS2, a gene implicated in diverse psychiatric and neurodevelopmental disorders impairs dendritic development and synaptic function. Nature Neuroscience (in revision).

Loe-Mie Y et al. SMARCA2 and other genome-wide supported schizophrenia-associated genes: regulation by REST/NRSF, network organization and primate-specific evolution.Hum Mol Genet. 2010 Jul 15;19(14):2841-57.

Purper-Ouakil D et al. Neurobiology of attention deficit/hyperactivity disorder. Pediatr Res. 2011 69R-76R.

Lepagnol-Bestel AM et al. Association of DISC1 gene with schizophrenia in families from two distinct French and Algerian populations. Psychiatr Genet. 2010 Dec;20(6):298-303.

Moalic JM et al. Primate‐Accelerated Evolutionary Genes: Novel Routes to Drug Discovery in Psychiatric Disorders Curr Med Chem. 2010 1300-16.