UMR 8250

Membrane Transport

Domaine de recherche principal: 

Neuropharmacology / cell signaling

Mots clefs: 

Optogenetics
Transporter
Synaptic vesicle
Lysosome
Neurodegeneration

Labelisation ENP: 

2014

Centre de recherche / Institut: 

INCC - UMR 8002 - Paris Descartes University

Code unité de recherche: 

UMR 8250

RESEARCH INTERESTS:

Leader

Leader: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Descartes
Laboratory

Nom: 

Neurophotonics laboratory
Publications

publications: 

Chapel A, Kieffer-Jaquinod S, Sagné C#, Verdon Q#, Ivaldi C, Mellal M, Thirion J, Jadot M, Bruley C, Garin J, Gasnier B#, Journet  A (2013) An extended proteome map of the lysosomal membrane reveals novel potential transporters. Mol Cell Proteomics, 12:1572

M. Martineau, T. Shi, J. Puyal, A.M. Knolhoff, J. Dulong, B. Gasnier#, J. Klingauf, J. V. Sweedler, R. Jahn, and J-P Mothet (2013) Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci, 34:646.

A. Jezegou#, E. Llinares, C. Anne#, S. Kieffer-Jaquinod, S. O'Regan#, J. Aupetit , A. Chabli, C. Sagné#, C. Debacker#, B. Chadefaux-Vekemans, A. Journet, B. Andre, B. Gasnier# (2012) Heptahelical protein PQLC2 is a lysosomal cationic amino acid exporter underlying the action of cysteamine in cystinosis therapy. PNAS 109:E3434

R. Ruivo#, G.C. Bellenchi#, X. Chen#, G. Zifarelli, C. Sagné#, C. Debacker#, M. Pusch, S. Supplisson, B. Gasnier# (2012) Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin, PNAS 109:E210.

N. Pietrancosta*, C. Anne*#, H. Prescher, R. Ruivo#, C. Sagné#, C. Debacker#, H.O. Bertrand, R. Brossmer, F. Acher, B. Gasnier# (2012) Successful prediction of the substrate-binding pocket in the SLC17 transporter sialin, JBC 287:11489

Microscopie à modulation de front d'onde

Domaine de recherche principal: 

Neurophysiology / systems neuroscience

Labelisation ENP: 

2007

Centre de recherche / Institut: 

INCC - UMR 8002 - Paris Descartes University

Code unité de recherche: 

UMR 8250

Le travail de recherche de cette équipe est consacré au développement de techniques optiques reposant sur la microscopie à modulation de front d’onde. Les montages optiques traditionnels utilisent des lentilles, diaphragmes, miroirs, réseaux ainsi que des fibres. Ces optiques ne permettent cependant que d’obtenir des faisceaux de formes relativement simples et ne peuvent être aisément déplacés au cours d’une expérience.

Leader

Leader: 

Établissements

Établissement de rattachement: 

CNRS

Établissements affiliés: 

Université Paris Descartes

Université: 

Université Paris Descartes
Laboratory

Nom: 

Neurophotonics laboratory
Publications

publications: 

Szabo V, Ventalon C, De Sars V, Bradley J, Emiliani V. Spatially selective holographic photoactivation and functional fluorescence imaging in freely behaving mice with a fiberscope. Neuron. 2014 Dec 17;84(6):1157-69. doi: 10.1016/j.neuron.2014.11.005. Epub 2014 Nov 26.

Lauterbach MA, Guillon M, Soltani A, Emiliani V. STED microscope with spiral phase contrast. Sci Rep. 2013;3:2050. doi: 10.1038/srep02050.

E. Papagiakoumou, A. Bègue, B. Leshem, O. Schwartz, B. Stell, J. Bradley, D. Oron and V. Emiliani. Functional patterned multiphoton excitation deep inside scattering tissue, Nature Photonics , in press.


    K. Kam, J.W. Worrell1, C. Ventalon, V. Emiliani, and J. L. Feldman. Emergence of population bursts from simultaneous activation of small subsets of preBötzinger Complex inspiratory neurons , Journal of Neuroscience, 33: 3332-3338 (2013) 


    E. Ronzitti, M. Guillon, V. de Sars, and V. Emiliani. LCoS nematic SLM characterization and modeling for diffraction efficiency optimization, zero and ghost orders suppression, Optics Express vol. 20, pp. 2012 (2012)


    A. Vaziri, and V. Emiliani. Reshaping the optical dimension in optogenetics, Current Opinion in Neurobiology vol. 22, pp. 2012 (2012)


    D. Oron, E. Papagiakoumou, F. Anselmi and V. Emiliani. Two-photon Optogenetics, Progress in Brain Research vol. 194, pp. 2012 (2012)


    F. Anselmi, C. Ventalon, V. de Sars, D. Ogden and V. Emiliani. 3D imaging and photostimulation by remote focusing and holographic light patterning, PNAS vol. 108, pp. 2011 (2011)


    S. Yang, E. Papagiakoumou, M. Guillon, V. de Sars, C.-M Tang, and V. Emiliani. 3D Holographic Photostimulation of the Dendritic Arbor, Journal of Neural engineering , 8: 046002 (2011)

Scanless two-photon excitation of channelrhodopsin-2, E. Papagiakoumou, F. Anselmi, A. Begue, V. de Sars, J. Gluckstad, E. Y. Isacoff, and V. Emiliani, Nature Methods 7, 848-854 (2010).

Holographic photolysis for multiple cell stimulation in mouse hippocampal slices, M. Zahid, M. Velez-Fort, E. Papagiakoumou, C. Ventalon, M. C. Angulo, and V. Emiliani, PLoS One 5, e9431 (2010).

Temporal focusing with spatially modulated excitation.E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, Optics Express 17, 5391-5401 (2009).

Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses, E. Papagiakoumou, C. Lutz, V. De Sars, D. Oron, and V. Emiliani, Optics Express 16, 22039-22047 (2008).

Holographic photolysis of caged neurotransmittersC. Lutz, T. Otis, V. DeSars, S. Charpak, D. Digregorio, and V. Emiliani, Nature Methods 5, 821-827 (2008).