Team
Biographie / Publications
Carsten Janke, DR1 CNRS, Senior Group leader Institut Curie
Education:
1993-1994: Diploma in Protein Biochemistry at the University of Leipzig, T. Arendt lab, Leipzig, Germany
1994-1998: PhD in Protein Biochemistry and Neurochemistry at the University of Leipzig, T. Arendt lab, Leipzig, Germany
Research activities:
1998-1999: Postdoc in DNA Biophysics at the CBM, CNRS, M. Leng lab, Orléans, France
1999-2002: Postdoc in Molecular and Cell Biology of Yeast, Cancer Research UK, E. Schiebel lab, Glasgow, UK
2002-2005: Postdoc and staff scientist in Biochemistry at the CRBM, CNRS, B. Eddé lab, Montpellier, France
2005-2010: Junior Group Leader at the CRBM, CNRS, Montpellier, France
2010-today: Senior Group Leader at the Institut Curie, Orsay/Paris, France
Distinctions:
2007: selected EMBO young investigator (EMBO YIP)
2014: elected EMBO member
Conference Organization:
- Initiator and main organizer of the EMBO conference series on Microtubules in Heidelberg (2010, 2012, 2014)
- Organizer of EMBL/EMBO symposia on Microtubules in Heidelberg (2016, 2018, 2020)
- Organizer of a CEFIPRA-funded Indian-French workshop “Frontiers in cytoskeleton research: coordination, adaptation, fine-tuning” (2015) in Pune, India
- Organizer of an EMBO workshop “Frontiers in cytoskeleton research” (2017) in Pune, India
- Organiser of the EMBO workshop “Emerging concepts of the neuronal cytoskeleton” (2017) in Puerto Varas, Chile
- Organiser of the Company of Biologists workshop “The cytoskeletal road to neuronal function” (2020) in Wiston House, West Sussex, UK
Teaching:
- Lecturer at the EMBO YIP PhD courses
- Lecturer in courses at different master programs of the Ecole Normale Supérieur, Institut Curie, Institut Pasteur and Universities in Paris
- Lecturer at international courses organized by the Institut Curie and the Institut Pasteur
- Lecturer at the international Cell Biology course in Valdivia, Chile (2014)
Selected activities:
- Regular participations as invited speakers at international conferences
- Multiple international and national scientific collaboration
- Coordinator of different international scientific projects
- Reviewer for international journals and grant agencies
Publications:
- Stoupa A, Adam F, Kariyawasam D, Strassel C, Gawade S, Szinnai G, Kauskot A, Lasne D, Janke C, Natarajan K, Schmitt A, Bole‐Feysot C, Nitschke P, Léger J, Jabot‐Hanin F, Tores F, Michel A, Munnich A, Besmond C, Scharfmann R, Lanza F, Borgel D, Polak M, Carré A (2018) TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology. EMBO Mol Med:e9569
- Silva CG, Peyre E, Adhikari MH, Tielens S, Tanco S, Van Damme P, Magno L, Krusy N, Agirman G, Magiera MM, Kessaris N, Malgrange B, Andrieux A, Janke C, Nguyen L (2018) Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis. Cell172:1063-1078
- 3. Shashi V, Magiera MM et la., Janke C#, Senderek J#(2018) Loss of tubulin deglutamylase CCP1 causes infantile‐onset neurodegeneration. EMBO J
- Munoz-Castaneda R, Diaz D, Peris L, Andrieux A, Bosc C, Munoz-Castaneda JM, Janke C, Alonso JR, Moutin M-J, Weruaga E (2018) Cytoskeleton stability is essential for the integrity of the cerebellum and its motor- and affective-related behaviors. Sci Rep8:3072
- Magiera MM, Singh P, Janke C (2018) SnapShot: Functions of Tubulin Posttranslational Modifications. Cell173:1552-1552 e1551
- Magiera MM, Singh P, Gadadhar S, Janke C (2018) Tubulin Posttranslational Modifications and Emerging Links to Human Disease. Cell173:1323-1327
- 7. Magiera MM, Bodakuntla S, Žiak J, Lacomme S, Marques Sousa P, Leboucher S, Hausrat TJ, Bosc C, Andrieux A, Kneussel M, Landry M, Calas A, Balastik M, Janke C (2018) Excessive tubulin polyglutamylation causes neurodegeneration and perturbs neuronal transport. EMBO J
- Janke C (2018) A unified reviewing format for grant applications and evaluations. EMBO Rep19:187-188
- Chabrier R, Janke C (2018) The comeback of hand drawing in modern life sciences. Nat Rev Mol Cell Biol19:137-138
- Basnet N, Nedozralova H, Crevenna AH, Bodakuntla S, Schlichthaerle T, Taschner M, Cardone G, Janke C, Jungmann R, Magiera MM, Biertumpfel C, Mizuno N (2018) Direct induction of microtubule branching by microtubule nucleation factor SSNA1. Nat Cell Biol20:1172-1180
- Natarajan K, Gadadhar S, Souphron J, Magiera MM, Janke C (2017) Molecular interactions between tubulin tails and glutamylases reveal determinants of glutamylation patterns. EMBO Rep18:1013-1026
- Janke C, Montagnac G (2017) Causes and Consequences of Microtubule Acetylation. Curr Biol27:R1287-R1292
- Gadadhar S, Dadi H, Bodakuntla S, Schnitzler A, Bieche I, Rusconi F, Janke C (2017) Tubulin glycylation controls primary cilia length. J Cell Biol216:2701-2713
- Gadadhar S, Bodakuntla S, Natarajan K, Janke C (2017) The tubulin code at a glance. J Cell Sci130:1347-1353
- Bosch Grau M, Masson C, Gadadhar S, Rocha C, Tort O, Marques Sousa P, Vacher S, Bieche I, Janke C (2017) Alterations in the balance of tubulin glycylation and glutamylation in photoreceptors leads to retinal degeneration. J Cell Sci130:938-949
- Belvindrah R, Natarajan K, Shabajee P, Bruel-Jungerman E, Bernard J, Goutierre M, Moutkine I, Jaglin XH, Savariradjane M, Irinopoulou T, Poncer J-C, Janke C, Francis F (2017) Mutation of the alpha-tubulin Tuba1a leads to straighter microtubules and perturbs neuronal migration. J Cell Biol216:2443-2461
- Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, Janke C, Schultz RM, Lampson MA (2017) Spindle asymmetry drives non-Mendelian chromosome segregation. Science358:668-672
- Akendengue L, Trepout S, Grana M, Voegele A, Janke C, Raynal B, Chenal A, Marco S, Wehenkel AM (2017) Bacterial kinesin light chain (Bklc) links the Btub cytoskeleton to membranes. Sci Rep7:45668
- Nirschl JJ, Magiera MM, Lazarus JE, Janke C, Holzbaur ELF (2016) alpha-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons. Cell Rep:celrep 2509
- Ly N, Elkhatib N, Bresteau E, Pietrement O, Khaled M, Magiera MM, Janke C, Le Cam E, Rutenberg AD, Montagnac G (2016) alphaTAT1 controls longitudinal spreading of acetylation marks from open microtubules extremities. Sci Rep6:35624
- Chakraborti S, Natarajan K, Curiel J, Janke C, Liu J (2016) The emerging role of the tubulin code: From the tubulin molecule to neuronal function and disease. Cytoskeleton (Hoboken)73:521-550
- Borisy G, Heald R, Howard J, Janke C, Musacchio A, Nogales E (2016) Microtubules: 50 years on from the discovery of tubulin. Nat Rev Mol Cell Biol17:322-328
- Blosa M, Bursch C, Weigel S, Holzer M, Jäger C, Janke C, Matthews RT, Arendt T, Morawski M (2016) Reorganization of Synaptic Connections and Perineuronal Nets in the Deep Cerebellar Nuclei of Purkinje Cell Degeneration Mutant Mice. Neural Plast2016:2828536
- Aillaud C, Bosc C, Saoudi Y, Denarier E, Peris L, Sago L, Taulet N, Cieren A, Tort O, Magiera MM, Janke C, Redeker V, Andrieux A, Moutin M-J (2016) Evidence for new C-terminally truncated variants of alpha- and beta-tubulins. Mol Biol Cell27:640-653
- Janke C, Steinmetz MO (2015) Optochemistry to control the microtubule cytoskeleton. EMBO J34:2114-2116
- Delgehyr N, Meunier A, Faucourt M, Bosch Grau M, Strehl L, Janke C, Spassky N (2015) Ependymal cell differentiation, from monociliated to multiciliated cells. Methods Cell Biol127:19-35
- Casanova M, de Monbrison F, van Dijk J, Janke C, Pages M, Bastien P (2015) Characterisation of polyglutamylases in trypanosomatids. Int J Parasitol45:121-132
- Barisic M, Silva e Sousa R, Tripathy SK, Magiera MM, Zaytsev AV, Pereira AL, Janke C, Grishchuk EL, Maiato H (2015) Microtubule detyrosination guides chromosomes during mitosis. Science348:799-803
- Wehenkel A, Janke C (2014) Towards elucidating the tubulin code. Nat Cell Biol16:303-305
- Tort O, Tanco S, Rocha C, Bieche I, Seixas C, Bosc C, Andrieux A, Moutin M-J, Xavier Aviles F, Lorenzo J, Janke C (2014) The cytosolic carboxypeptidases CCP2 and CCP3 catalyze posttranslational removal of acidic amino acids. Mol Biol Cell25:3017-3027
- Rocha C, Papon L, Cacheux W, Marques Sousa P, Lascano V, Tort O, Giordano T, Vacher S, Lemmers B, Mariani P, Meseure D, Medema JP, Bièche I, Hahne M, Janke C (2014) Tubulin glycylases are required for primary cilia, control of cell proliferation and tumor development in colon. EMBO J33:2247-2260
- Mendes Maia T, Gogendeau D, Pennetier C, Janke C, Basto R (2014) Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules. Biol Open3:138-151
- Magiera MM, Janke C (2014) Post-translational modifications of tubulin. Curr Biol24:R351-R354
- Janke C (2014) The tubulin code: Molecular components, readout mechanisms, and functions. J Cell Biol206:461-472
- Prota AE, Magiera MM, Kuijpers M, Bargsten K, Frey D, Wieser M, Jaussi R, Hoogenraad CC, Kammerer RA, Janke C, Steinmetz MO (2013) Structural basis of tubulin tyrosination by tubulin tyrosine ligase. J Cell Biol200:259-270
- Magiera MM, Janke C (2013) Investigating tubulin posttranslational modifications with specific antibodies. In Methods Cell Biol, Correia JJ, Wilson L (eds), Vol. 115, 2013/08/27 edn, pp 247-267. Burlington: Academic Press
- Janke C (2013) Mysterious modification of tubulin. Nat Rev Mol Cell Biol14:692
- Bosch Grau M, Gonzalez Curto G, Rocha C, Magiera MM, Marques Sousa P, Giordano T, Spassky N, Janke C (2013) Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia. J Cell Biol202:441-451
- Castro-Castro A, Janke C, Montagnac G, Paul-Gilloteaux P, Chavrier P (2012) ATAT1/MEC-17 acetyltransferase and HDAC6 deacetylase control a balance of acetylation of alpha-tubulin and cortactin and regulate MT1-MMP trafficking and breast tumor cell invasion. Eur J Cell Biol91:950-960
- Moutin M-J, Andrieux A, Janke C (2011) [Microtubule polyglutamylation and neurodegeneration.]. Med Sci (Paris)27:464-467
- Lacroix B, Janke C (2011) Generation of differentially polyglutamylated microtubules. Methods Mol Biol777:57-69
- Janke C, Bulinski JC (2011) Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions. Nat Rev Mol Cell Biol12:773-786
- Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme J-C, Bosson A, Peris L, Gold ND, Lacroix B, Bosch Grau M, Bec N, Larroque C, Desagher S, Holzer M, Andrieux A, Moutin M-J, Janke C (2010) A family of protein-deglutamylating enzymes associated with neurodegeneration. Cell143:564-578
- Lacroix B, van Dijk J, Gold ND, Guizetti J, Aldrian-Herrada G, Rogowski K, Gerlich DW, Janke C (2010) Tubulin polyglutamylation stimulates spastin-mediated microtubule severing. J Cell Biol189:945-954
- Kashiwaya K, Nakagawa H, Hosokawa M, Mochizuki Y, Ueda K, Piao L, Chung S, Hamamoto R, Eguchi H, Ohigashi H, Ishikawa O, Janke C, Shinomura Y, Nakamura Y (2010) Involvement of the tubulin tyrosine ligase-like family member 4 polyglutamylase in PELP1 polyglutamylation and chromatin remodeling in pancreatic cancer cells.Cancer Res70:4024-4033
- Janke C, Kneussel M (2010) Tubulin post-translational modifications: encoding functions on the neuronal microtubule cytoskeleton. Trends Neurosci33:362-372
- Wloga D, Webster DM, Rogowski K, Bré MH, Levilliers N, Jerka-Dziadosz M, Janke C, Dougan ST, Gaertig J (2009) TTLL3 Is a tubulin glycine ligase that regulates the assembly of cilia. Dev Cell16:867-876
- Rogowski K, Juge F, van Dijk J, Wloga D, Strub J-M, Levilliers N, Thomas D, Bré MH, Van Dorsselaer A, Gaertig J, Janke C (2009) Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation. Cell137:1076-1087
- Maas C, Belgardt D, Lee HK, Heisler FF, Lappe-Siefke C, Magiera MM, van Dijk J, Hausrat TJ, Janke C, Kneussel M (2009) Synaptic activation modifies microtubules underlying transport of postsynaptic cargo. Proc Natl Acad Sci U S A106:8731-8736
- Wloga D, Rogowski K, Sharma N, Van Dijk J, Janke C, Edde B, Bré MH, Levilliers N, Redeker V, Duan J, Gorovsky MA, Jerka-Dziadosz M, Gaertig J (2008) Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophila. Eukaryot Cell7:1362-1372
- van Dijk J, Miro J, Strub J-M, Lacroix B, van Dorsselaer A, Eddé B, Janke C (2008) Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates. J Biol Chem283:3915-3922
- Janke C, Rogowski K, van Dijk J (2008) Polyglutamylation: a fine-regulator of protein function? 'Protein Modifications: beyond the usual suspects' review series. EMBO Rep9:636-641
- Bieling P, Kandels-Lewis S, Telley IA, van Dijk J, Janke C, Surrey T (2008) CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites. J Cell Biol183:1223-1233
- van Dijk J, Rogowski K, Miro J, Lacroix B, Eddé B, Janke C (2007) A targeted multienzyme mechanism for selective microtubule polyglutamylation. Mol Cell26:437-448
- Ikegami K, Heier RL, Taruishi M, Takagi H, Mukai M, Shimma S, Taira S, Hatanaka K, Morone N, Yao I, Campbell PK, Yuasa S, Janke C, Macgregor GR, Setou M (2007) Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function. Proc Natl Acad Sci U S A104:3213–3218
- Janke C, Rogowski K, Wloga D, Regnard C, Kajava AV, Strub J-M, Temurak N, van Dijk J, Boucher D, van Dorsselaer A, Suryavanshi S, Gaertig J, Eddé B (2005) Tubulin polyglutamylase enzymes are members of the TTL domain protein family. Science308:1758-1762
- Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast21:947-962
- Scharfenberger M, Ortiz J, Grau N, Janke C, Schiebel E, Lechner J (2003) Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex. EMBO J22:6584-6597
- Regnard C, Fesquet D, Janke C, Boucher D, Desbruyeres E, Koulakoff A, Insina C, Travo P, Eddé B (2003) Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase. J Cell Sci116:4181-4190
- Janke C, Martin D, Giraud-Panis M-J, Decoville M, Locker D (2003) Drosophila DSP1 and rat HMGB1 have equivalent DNA binding properties and share a similar secondary fold. J Biochem (Tokyo)133:533-539
- Tanaka TU, Rachidi N, Janke C, Pereira G, Galova M, Schiebel E, Stark MJR, Nasmyth K (2002) Evidence that the Ipl1-Sli15 (Aurora Kinase-INCENP) Complex Promotes Chromosome Bi-orientation by Altering Kinetochore-Spindle Pole Connections. Cell108:317-329.
- Janke C, Ortiz J, Tanaka TU, Lechner J, Schiebel E (2002) Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. EMBO J21:181-193
- Schramm C, Janke C, Schiebel E (2001) Molecular dissection of yeast spindle pole bodies by two hybrid, in vitro binding, and co-purification. Methods Cell Biol67:71-94
- Janke C, Ortiz J, Lechner J, Shevchenko A, Shevchenko A, Magiera MM, Schramm C, Schiebel E (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J20:777-791
- Holzer M, Rodel L, Seeger G, Gartner U, Narz F, Janke C, Heumann R, Arendt T (2001) Activation of mitogen-activated protein kinase cascade and phosphorylation of cytoskeletal proteins after neurone-specific activation of p21ras. II. Cytoskeletal proteins and dendritic morphology. Neuroscience105:1041-1054
- Janke C, Beck M, Holzer M, Bigl V, Arendt T (2000) Analysis of the molecular heterogeneity of the microtubule-associated protein tau by two-dimensional electrophoresis and RT-PCR. Brain Res Brain Res Protoc5:231-242
- Janke C, Beck M, Stahl T, Holzer M, Brauer K, Bigl V, Arendt T (1999) Phylogenetic diversity of the expression of the microtubule-associated protein tau: implications for neurodegenerative disorders. Brain Res Mol Brain Res68:119-128
- Mack AF, Germer A, Janke C, Reichenbach A (1998) Muller (glial) cells in the teleost retina: consequences of continuous growth. Glia22:306-313
- Janke C, Gartner U, Holzer M, Arendt T (1998) Reversible in vivo phosphorylation of tau induced by okadaic acid and by unspecific brain lesion in rat. J Hirnforsch39:143-153
- Gärtner U, Janke C, Holzer M, Vanmechelen E, Arendt T (1998) Postmortem changes in the phosphorylation state of tau-protein in the rat brain. Neurobiol Aging19:535-543
- Arendt T, Holzer M, Bruckner MK, Janke C, Gartner U (1998) The use of okadaic acid in vivo and the induction of molecular changes typical for Alzheimer's disease. Neuroscience85:1337-1340
- Janke C, Holzer M, Klose J, Arendt T (1996) Distribution of isoforms of the microtubule-associated protein tau in grey and white matter areas of human brain: a two-dimensional gelelectrophoretic analysis. FEBS Lett379:222-226