Carsten Janke, DR1 CNRS, Senior Group leader Institut Curie

Education:

1993-1994: Diploma in Protein Biochemistry at the University of Leipzig, T. Arendt lab, Leipzig, Germany

1994-1998: PhD in Protein Biochemistry and Neurochemistry at the University of Leipzig, T. Arendt lab, Leipzig, Germany

Research activities:

1998-1999: Postdoc in DNA Biophysics at the CBM, CNRS, M. Leng lab, Orléans, France

1999-2002: Postdoc in Molecular and Cell Biology of Yeast, Cancer Research UK, E. Schiebel lab, Glasgow, UK

2002-2005: Postdoc and staff scientist in Biochemistry at the CRBM, CNRS, B. Eddé lab, Montpellier, France

2005-2010: Junior Group Leader at the CRBM, CNRS, Montpellier, France

2010-today: Senior Group Leader at the Institut Curie, Orsay/Paris, France

Distinctions:

2007: selected EMBO young investigator (EMBO YIP)

2014: elected EMBO member

Selected activities:

-       Regular participations as invited speakers at international conferences

-       Multiple international and national scientific collaborations

-       Initiator and main organizer of the EMBO conference series on Microtubules in Heidelberg (2010, 2012, 2014)

-       Organizer of a CEFIPRA-funded Indian-French workshop “Frontiers in cytoskeleton research: coordination, adaptation, fine-tuning” (2015) in Pune, India

-       Organizer of an EMBL-EMBO symposium on Microtubules in Heidelberg (2016)

-       Annual lecturer at the EMBO YIP PhD courses

-       Lecturer in courses at different master programs of the Ecole Normale Supérieur, Institut Curie, Institut Pasteur and Universities in Paris

-       Lecturer at international courses organized by the Institut Curie and the Institut Pasteur

-       Lecturer at the international Cell Biology course in Valdivia, Chile (2014)

-       Coordinator of different international scientific projects

-       Reviewer for international journals and grant agencies

Publications:

1. Wehenkel A, Janke C (2014) Towards elucidating the tubulin code. Nat Cell Biol 16: 303-305
2. Tort O, Tanco S, Rocha C, Bieche I, Seixas C, Bosc C, Andrieux A, Moutin M-J, Xavier Aviles F, Lorenzo J, Janke C (2014) The cytosolic carboxypeptidases CCP2 and CCP3 catalyze posttranslational removal of acidic amino acids. Mol Biol Cell 25: 3017-3027
3. Rocha C, Papon L, Cacheux W, Marques Sousa P, Lascano V, Tort O, Giordano T, Vacher S, Lemmers B, Mariani P, Meseure D, Medema JP, Bièche I, Hahne M, Janke C (2014) Tubulin glycylases are required for primary cilia, control of cell proliferation and tumor development in colon. EMBO J 33: 2247-2260
4. Mendes Maia T, Gogendeau D, Pennetier C, Janke C, Basto R (2014) Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules. Biol Open 3: 138-151
5. Magiera MM, Janke C (2014) Post-translational modifications of tubulin. Curr Biol 24: R351-R354
6. Janke C (2014) The tubulin code: Molecular components, readout mechanisms, and functions. J Cell Biol 206: 461-472
7. 7.     Casanova M, de Monbrison F, van Dijk J, Janke C, Pages M, Bastien P (2014) Characterisation of polyglutamylases in trypanosomatids. Int J Parasitol
8. Prota AE, Magiera MM, Kuijpers M, Bargsten K, Frey D, Wieser M, Jaussi R, Hoogenraad CC, Kammerer RA, Janke C, Steinmetz MO (2013) Structural basis of tubulin tyrosination by tubulin tyrosine ligase. J Cell Biol 200: 259-270
9. Magiera MM, Janke C (2013) Investigating tubulin posttranslational modifications with specific antibodies. In Methods Cell Biol, Correia JJ, Wilson L (eds), Vol. 115, 2013/08/27 edn, pp 247-267. Burlington: Academic Press
10. Janke C (2013) Mysterious modification of tubulin. Nat Rev Mol Cell Biol 14: 692
11. Bosch Grau M, Gonzalez Curto G, Rocha C, Magiera MM, Marques Sousa P, Giordano T, Spassky N, Janke C (2013) Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia. J Cell Biol 202: 441-451
12. Castro-Castro A, Janke C, Montagnac G, Paul-Gilloteaux P, Chavrier P (2012) ATAT1/MEC-17 acetyltransferase and HDAC6 deacetylase control a balance of acetylation of alpha-tubulin and cortactin and regulate MT1-MMP trafficking and breast tumor cell invasion. Eur J Cell Biol 91: 950-960
13. Moutin M-J, Andrieux A, Janke C (2011) [Microtubule polyglutamylation and neurodegeneration.]. Med Sci (Paris) 27: 464-467
14. Lacroix B, Janke C (2011) Generation of differentially polyglutamylated microtubules. Methods Mol Biol 777: 57-69
15. Janke C, Bulinski JC (2011) Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions. Nat Rev Mol Cell Biol 12: 773-786
16. Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme J-C, Bosson A, Peris L, Gold ND, Lacroix B, Bosch Grau M, Bec N, Larroque C, Desagher S, Holzer M, Andrieux A, Moutin M-J, Janke C (2010) A family of protein-deglutamylating enzymes associated with neurodegeneration. Cell 143: 564-578
17. Lacroix B, van Dijk J, Gold ND, Guizetti J, Aldrian-Herrada G, Rogowski K, Gerlich DW, Janke C (2010) Tubulin polyglutamylation stimulates spastin-mediated microtubule severing. J Cell Biol 189: 945-954
18. Kashiwaya K, Nakagawa H, Hosokawa M, Mochizuki Y, Ueda K, Piao L, Chung S, Hamamoto R, Eguchi H, Ohigashi H, Ishikawa O, Janke C, Shinomura Y, Nakamura Y (2010) Involvement of the tubulin tyrosine ligase-like family member 4 polyglutamylase in PELP1 polyglutamylation and chromatin remodeling in pancreatic cancer cells. Cancer Res 70: 4024-4033
19. Janke C, Kneussel M (2010) Tubulin post-translational modifications: encoding functions on the neuronal microtubule cytoskeleton. Trends Neurosci 33: 362-372
20. Wloga D, Webster DM, Rogowski K, Bré MH, Levilliers N, Jerka-Dziadosz M, Janke C, Dougan ST, Gaertig J (2009) TTLL3 Is a tubulin glycine ligase that regulates the assembly of cilia. Dev Cell 16: 867-876
21. Rogowski K, Juge F, van Dijk J, Wloga D, Strub J-M, Levilliers N, Thomas D, Bré MH, Van Dorsselaer A, Gaertig J, Janke C (2009) Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation. Cell 137: 1076-1087
22. Maas C, Belgardt D, Lee HK, Heisler FF, Lappe-Siefke C, Magiera MM, van Dijk J, Hausrat TJ, Janke C, Kneussel M (2009) Synaptic activation modifies microtubules underlying transport of postsynaptic cargo. Proc Natl Acad Sci U S A 106: 8731-8736
23. Wloga D, Rogowski K, Sharma N, Van Dijk J, Janke C, Edde B, Bré MH, Levilliers N, Redeker V, Duan J, Gorovsky MA, Jerka-Dziadosz M, Gaertig J (2008) Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophila. Eukaryot Cell 7: 1362-1372
24. van Dijk J, Miro J, Strub J-M, Lacroix B, van Dorsselaer A, Eddé B, Janke C (2008) Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates. J Biol Chem 283: 3915-3922
25. Janke C, Rogowski K, van Dijk J (2008) Polyglutamylation: a fine-regulator of protein function? 'Protein Modifications: beyond the usual suspects' review series. EMBO Rep 9: 636-641
26. Bieling P, Kandels-Lewis S, Telley IA, van Dijk J, Janke C, Surrey T (2008) CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites. J Cell Biol 183: 1223-1233
27. van Dijk J, Rogowski K, Miro J, Lacroix B, Eddé B, Janke C (2007) A targeted multienzyme mechanism for selective microtubule polyglutamylation. Mol Cell 26: 437-448
28. Ikegami K, Heier RL, Taruishi M, Takagi H, Mukai M, Shimma S, Taira S, Hatanaka K, Morone N, Yao I, Campbell PK, Yuasa S, Janke C, Macgregor GR, Setou M (2007) Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function. Proc Natl Acad Sci U S A 104: 3213–3218
29. Janke C, Rogowski K, Wloga D, Regnard C, Kajava AV, Strub J-M, Temurak N, van Dijk J, Boucher D, van Dorsselaer A, Suryavanshi S, Gaertig J, Eddé B (2005) Tubulin polyglutamylase enzymes are members of the TTL domain protein family. Science 308: 1758-1762
30. Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21: 947-962
31. Scharfenberger M, Ortiz J, Grau N, Janke C, Schiebel E, Lechner J (2003) Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex. EMBO J 22: 6584-6597
32. Regnard C, Fesquet D, Janke C, Boucher D, Desbruyeres E, Koulakoff A, Insina C, Travo P, Eddé B (2003) Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase. J Cell Sci 116: 4181-4190
33. Janke C, Martin D, Giraud-Panis M-J, Decoville M, Locker D (2003) Drosophila DSP1 and rat HMGB1 have equivalent DNA binding properties and share a similar secondary fold. J Biochem (Tokyo) 133: 533-539
34. Tanaka TU, Rachidi N, Janke C, Pereira G, Galova M, Schiebel E, Stark MJR, Nasmyth K (2002) Evidence that the Ipl1-Sli15 (Aurora Kinase-INCENP) Complex Promotes Chromosome Bi-orientation by Altering Kinetochore-Spindle Pole Connections. Cell 108: 317-329.
35. Janke C, Ortiz J, Tanaka TU, Lechner J, Schiebel E (2002) Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. EMBO J 21: 181-193
36. Schramm C, Janke C, Schiebel E (2001) Molecular dissection of yeast spindle pole bodies by two hybrid, in vitro binding, and co-purification. Methods Cell Biol 67: 71-94
37. Janke C, Ortiz J, Lechner J, Shevchenko A, Shevchenko A, Magiera MM, Schramm C, Schiebel E (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20: 777-791
38. Holzer M, Rodel L, Seeger G, Gartner U, Narz F, Janke C, Heumann R, Arendt T (2001) Activation of mitogen-activated protein kinase cascade and phosphorylation of cytoskeletal proteins after neurone-specific activation of p21ras. II. Cytoskeletal proteins and dendritic morphology. Neuroscience 105: 1041-1054
39. Janke C, Beck M, Holzer M, Bigl V, Arendt T (2000) Analysis of the molecular heterogeneity of the microtubule-associated protein tau by two-dimensional electrophoresis and RT-PCR. Brain Res Brain Res Protoc 5: 231-242
40. Janke C, Beck M, Stahl T, Holzer M, Brauer K, Bigl V, Arendt T (1999) Phylogenetic diversity of the expression of the microtubule-associated protein tau: implications for neurodegenerative disorders. Brain Res Mol Brain Res 68: 119-128
41. Mack AF, Germer A, Janke C, Reichenbach A (1998) Muller (glial) cells in the teleost retina: consequences of continuous growth. Glia 22: 306-313
42. Janke C, Gartner U, Holzer M, Arendt T (1998) Reversible in vivo phosphorylation of tau induced by okadaic acid and by unspecific brain lesion in rat. J Hirnforsch 39: 143-153
43. Gärtner U, Janke C, Holzer M, Vanmechelen E, Arendt T (1998) Postmortem changes in the phosphorylation state of tau-protein in the rat brain. Neurobiol Aging 19: 535-543
44. Arendt T, Holzer M, Bruckner MK, Janke C, Gartner U (1998) The use of okadaic acid in vivo and the induction of molecular changes typical for Alzheimer's disease. Neuroscience 85: 1337-1340
45. Janke C, Holzer M, Klose J, Arendt T (1996) Distribution of isoforms of the microtubule-associated protein tau in grey and white matter areas of human brain: a two-dimensional gelelectrophoretic analysis. FEBS Lett 379: 222-226