Monsieur
Carsten JANKE
Directeur de recherche
Chercheur
ED 426
10-02-2015
22-11-2018
27-09-2017
Available to host a PhD student

Team

Carsten JANKE
In our ongoing projects, we are using biochemistry, biophysics and structural biology in conjunction with cell and mouse biology to identify the molecular mechanisms by which tubulin...

Biographie / Publications

Carsten Janke, DR1 CNRS, Senior Group leader Institut Curie

Education:

1993-1994: Diploma in Protein Biochemistry at the University of Leipzig, T. Arendt lab, Leipzig, Germany

1994-1998: PhD in Protein Biochemistry and Neurochemistry at the University of Leipzig, T. Arendt lab, Leipzig, Germany

Research activities:

1998-1999: Postdoc in DNA Biophysics at the CBM, CNRS, M. Leng lab, Orléans, France

1999-2002: Postdoc in Molecular and Cell Biology of Yeast, Cancer Research UK, E. Schiebel lab, Glasgow, UK

2002-2005: Postdoc and staff scientist in Biochemistry at the CRBM, CNRS, B. Eddé lab, Montpellier, France

2005-2010: Junior Group Leader at the CRBM, CNRS, Montpellier, France

2010-today: Senior Group Leader at the Institut Curie, Orsay/Paris, France

Distinctions:

2007: selected EMBO young investigator (EMBO YIP)

2014: elected EMBO member

Conference Organization:

- Initiator and main organizer of the EMBO conference series on Microtubules in Heidelberg (2010, 2012, 2014)

- Organizer of EMBL/EMBO symposia on Microtubules in Heidelberg (2016, 2018, 2020)

- Organizer of a CEFIPRA-funded Indian-French workshop “Frontiers in cytoskeleton research: coordination, adaptation, fine-tuning” (2015) in Pune, India

- Organizer of an EMBO workshop “Frontiers in cytoskeleton research” (2017) in Pune, India

- Organiser of the EMBO workshop “Emerging concepts of the neuronal cytoskeleton” (2017) in Puerto Varas, Chile

- Organiser of the Company of Biologists workshop “The cytoskeletal road to neuronal function” (2020) in Wiston House, West Sussex, UK

Teaching:

- Lecturer at the EMBO YIP PhD courses

- Lecturer in courses at different master programs of the Ecole Normale Supérieur, Institut Curie, Institut Pasteur and Universities in Paris

- Lecturer at international courses organized by the Institut Curie and the Institut Pasteur 

- Lecturer at the international Cell Biology course in Valdivia, Chile (2014)

Selected activities:

- Regular participations as invited speakers at international conferences

- Multiple international and national scientific collaboration

- Coordinator of different international scientific projects

- Reviewer for international journals and grant agencies

Publications: 

  1. Stoupa A, Adam F, Kariyawasam D, Strassel C, Gawade S, Szinnai G, Kauskot A, Lasne D, Janke C, Natarajan K, Schmitt A, Bole‐Feysot C, Nitschke P, Léger J, Jabot‐Hanin F, Tores F, Michel A, Munnich A, Besmond C, Scharfmann R, Lanza F, Borgel D, Polak M, Carré A (2018) TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology. EMBO Mol Med:e9569
  2. Silva CG, Peyre E, Adhikari MH, Tielens S, Tanco S, Van Damme P, Magno L, Krusy N, Agirman G, Magiera MM, Kessaris N, Malgrange B, Andrieux A, Janke C, Nguyen L (2018) Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis. Cell172:1063-1078
  3. 3.    Shashi V, Magiera MM et la., Janke C#, Senderek J#(2018) Loss of tubulin deglutamylase CCP1 causes infantile‐onset neurodegeneration. EMBO J
  4. Munoz-Castaneda R, Diaz D, Peris L, Andrieux A, Bosc C, Munoz-Castaneda JM, Janke C, Alonso JR, Moutin M-J, Weruaga E (2018) Cytoskeleton stability is essential for the integrity of the cerebellum and its motor- and affective-related behaviors. Sci Rep8:3072
  5. Magiera MM, Singh P, Janke C (2018) SnapShot: Functions of Tubulin Posttranslational Modifications. Cell173:1552-1552 e1551
  6. Magiera MM, Singh P, Gadadhar S, Janke C (2018) Tubulin Posttranslational Modifications and Emerging Links to Human Disease. Cell173:1323-1327
  7. 7.    Magiera MM, Bodakuntla S, Žiak J, Lacomme S, Marques Sousa P, Leboucher S, Hausrat TJ, Bosc C, Andrieux A, Kneussel M, Landry M, Calas A, Balastik M, Janke C (2018) Excessive tubulin polyglutamylation causes neurodegeneration and perturbs neuronal transport. EMBO J
  8. Janke C (2018) A unified reviewing format for grant applications and evaluations. EMBO Rep19:187-188
  9. Chabrier R, Janke C (2018) The comeback of hand drawing in modern life sciences. Nat Rev Mol Cell Biol19:137-138
  10. Basnet N, Nedozralova H, Crevenna AH, Bodakuntla S, Schlichthaerle T, Taschner M, Cardone G, Janke C, Jungmann R, Magiera MM, Biertumpfel C, Mizuno N (2018) Direct induction of microtubule branching by microtubule nucleation factor SSNA1. Nat Cell Biol20:1172-1180
  11. Natarajan K, Gadadhar S, Souphron J, Magiera MM, Janke C (2017) Molecular interactions between tubulin tails and glutamylases reveal determinants of glutamylation patterns. EMBO Rep18:1013-1026
  12. Janke C, Montagnac G (2017) Causes and Consequences of Microtubule Acetylation. Curr Biol27:R1287-R1292
  13. Gadadhar S, Dadi H, Bodakuntla S, Schnitzler A, Bieche I, Rusconi F, Janke C (2017) Tubulin glycylation controls primary cilia length. J Cell Biol216:2701-2713
  14. Gadadhar S, Bodakuntla S, Natarajan K, Janke C (2017) The tubulin code at a glance. J Cell Sci130:1347-1353
  15. Bosch Grau M, Masson C, Gadadhar S, Rocha C, Tort O, Marques Sousa P, Vacher S, Bieche I, Janke C (2017) Alterations in the balance of tubulin glycylation and glutamylation in photoreceptors leads to retinal degeneration. J Cell Sci130:938-949
  16. Belvindrah R, Natarajan K, Shabajee P, Bruel-Jungerman E, Bernard J, Goutierre M, Moutkine I, Jaglin XH, Savariradjane M, Irinopoulou T, Poncer J-C, Janke C, Francis F (2017) Mutation of the alpha-tubulin Tuba1a leads to straighter microtubules and perturbs neuronal migration. J Cell Biol216:2443-2461
  17. Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, Janke C, Schultz RM, Lampson MA (2017) Spindle asymmetry drives non-Mendelian chromosome segregation. Science358:668-672
  18. Akendengue L, Trepout S, Grana M, Voegele A, Janke C, Raynal B, Chenal A, Marco S, Wehenkel AM (2017) Bacterial kinesin light chain (Bklc) links the Btub cytoskeleton to membranes. Sci Rep7:45668
  19. Nirschl JJ, Magiera MM, Lazarus JE, Janke C, Holzbaur ELF (2016) alpha-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons. Cell Rep:celrep 2509
  20. Ly N, Elkhatib N, Bresteau E, Pietrement O, Khaled M, Magiera MM, Janke C, Le Cam E, Rutenberg AD, Montagnac G (2016) alphaTAT1 controls longitudinal spreading of acetylation marks from open microtubules extremities. Sci Rep6:35624
  21. Chakraborti S, Natarajan K, Curiel J, Janke C, Liu J (2016) The emerging role of the tubulin code: From the tubulin molecule to neuronal function and disease. Cytoskeleton (Hoboken)73:521-550
  22. Borisy G, Heald R, Howard J, Janke C, Musacchio A, Nogales E (2016) Microtubules: 50 years on from the discovery of tubulin. Nat Rev Mol Cell Biol17:322-328
  23. Blosa M, Bursch C, Weigel S, Holzer M, Jäger C, Janke C, Matthews RT, Arendt T, Morawski M (2016) Reorganization of Synaptic Connections and Perineuronal Nets in the Deep Cerebellar Nuclei of Purkinje Cell Degeneration Mutant Mice. Neural Plast2016:2828536
  24. Aillaud C, Bosc C, Saoudi Y, Denarier E, Peris L, Sago L, Taulet N, Cieren A, Tort O, Magiera MM, Janke C, Redeker V, Andrieux A, Moutin M-J (2016) Evidence for new C-terminally truncated variants of alpha- and beta-tubulins. Mol Biol Cell27:640-653
  25. Janke C, Steinmetz MO (2015) Optochemistry to control the microtubule cytoskeleton. EMBO J34:2114-2116
  26. Delgehyr N, Meunier A, Faucourt M, Bosch Grau M, Strehl L, Janke C, Spassky N (2015) Ependymal cell differentiation, from monociliated to multiciliated cells. Methods Cell Biol127:19-35
  27. Casanova M, de Monbrison F, van Dijk J, Janke C, Pages M, Bastien P (2015) Characterisation of polyglutamylases in trypanosomatids. Int J Parasitol45:121-132
  28. Barisic M, Silva e Sousa R, Tripathy SK, Magiera MM, Zaytsev AV, Pereira AL, Janke C, Grishchuk EL, Maiato H (2015) Microtubule detyrosination guides chromosomes during mitosis. Science348:799-803
  29. Wehenkel A, Janke C (2014) Towards elucidating the tubulin code. Nat Cell Biol16:303-305
  30. Tort O, Tanco S, Rocha C, Bieche I, Seixas C, Bosc C, Andrieux A, Moutin M-J, Xavier Aviles F, Lorenzo J, Janke C (2014) The cytosolic carboxypeptidases CCP2 and CCP3 catalyze posttranslational removal of acidic amino acids. Mol Biol Cell25:3017-3027
  31. Rocha C, Papon L, Cacheux W, Marques Sousa P, Lascano V, Tort O, Giordano T, Vacher S, Lemmers B, Mariani P, Meseure D, Medema JP, Bièche I, Hahne M, Janke C (2014) Tubulin glycylases are required for primary cilia, control of cell proliferation and tumor development in colon. EMBO J33:2247-2260
  32. Mendes Maia T, Gogendeau D, Pennetier C, Janke C, Basto R (2014) Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules. Biol Open3:138-151
  33. Magiera MM, Janke C (2014) Post-translational modifications of tubulin. Curr Biol24:R351-R354
  34. Janke C (2014) The tubulin code: Molecular components, readout mechanisms, and functions. J Cell Biol206:461-472
  35. Prota AE, Magiera MM, Kuijpers M, Bargsten K, Frey D, Wieser M, Jaussi R, Hoogenraad CC, Kammerer RA, Janke C, Steinmetz MO (2013) Structural basis of tubulin tyrosination by tubulin tyrosine ligase. J Cell Biol200:259-270
  36. Magiera MM, Janke C (2013) Investigating tubulin posttranslational modifications with specific antibodies. In Methods Cell Biol, Correia JJ, Wilson L (eds), Vol. 115, 2013/08/27 edn, pp 247-267. Burlington: Academic Press
  37. Janke C (2013) Mysterious modification of tubulin. Nat Rev Mol Cell Biol14:692
  38. Bosch Grau M, Gonzalez Curto G, Rocha C, Magiera MM, Marques Sousa P, Giordano T, Spassky N, Janke C (2013) Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia. J Cell Biol202:441-451
  39. Castro-Castro A, Janke C, Montagnac G, Paul-Gilloteaux P, Chavrier P (2012) ATAT1/MEC-17 acetyltransferase and HDAC6 deacetylase control a balance of acetylation of alpha-tubulin and cortactin and regulate MT1-MMP trafficking and breast tumor cell invasion. Eur J Cell Biol91:950-960
  40. Moutin M-J, Andrieux A, Janke C (2011) [Microtubule polyglutamylation and neurodegeneration.]. Med Sci (Paris)27:464-467
  41. Lacroix B, Janke C (2011) Generation of differentially polyglutamylated microtubules. Methods Mol Biol777:57-69
  42. Janke C, Bulinski JC (2011) Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions. Nat Rev Mol Cell Biol12:773-786
  43. Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme J-C, Bosson A, Peris L, Gold ND, Lacroix B, Bosch Grau M, Bec N, Larroque C, Desagher S, Holzer M, Andrieux A, Moutin M-J, Janke C (2010) A family of protein-deglutamylating enzymes associated with neurodegeneration. Cell143:564-578
  44. Lacroix B, van Dijk J, Gold ND, Guizetti J, Aldrian-Herrada G, Rogowski K, Gerlich DW, Janke C (2010) Tubulin polyglutamylation stimulates spastin-mediated microtubule severing. J Cell Biol189:945-954
  45. Kashiwaya K, Nakagawa H, Hosokawa M, Mochizuki Y, Ueda K, Piao L, Chung S, Hamamoto R, Eguchi H, Ohigashi H, Ishikawa O, Janke C, Shinomura Y, Nakamura Y (2010) Involvement of the tubulin tyrosine ligase-like family member 4 polyglutamylase in PELP1 polyglutamylation and chromatin remodeling in pancreatic cancer cells.Cancer Res70:4024-4033
  46. Janke C, Kneussel M (2010) Tubulin post-translational modifications: encoding functions on the neuronal microtubule cytoskeleton. Trends Neurosci33:362-372
  47. Wloga D, Webster DM, Rogowski K, Bré MH, Levilliers N, Jerka-Dziadosz M, Janke C, Dougan ST, Gaertig J (2009) TTLL3 Is a tubulin glycine ligase that regulates the assembly of cilia. Dev Cell16:867-876
  48. Rogowski K, Juge F, van Dijk J, Wloga D, Strub J-M, Levilliers N, Thomas D, Bré MH, Van Dorsselaer A, Gaertig J, Janke C (2009) Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation. Cell137:1076-1087
  49. Maas C, Belgardt D, Lee HK, Heisler FF, Lappe-Siefke C, Magiera MM, van Dijk J, Hausrat TJ, Janke C, Kneussel M (2009) Synaptic activation modifies microtubules underlying transport of postsynaptic cargo. Proc Natl Acad Sci U S A106:8731-8736
  50. Wloga D, Rogowski K, Sharma N, Van Dijk J, Janke C, Edde B, Bré MH, Levilliers N, Redeker V, Duan J, Gorovsky MA, Jerka-Dziadosz M, Gaertig J (2008) Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophila. Eukaryot Cell7:1362-1372
  51. van Dijk J, Miro J, Strub J-M, Lacroix B, van Dorsselaer A, Eddé B, Janke C (2008) Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates. J Biol Chem283:3915-3922
  52. Janke C, Rogowski K, van Dijk J (2008) Polyglutamylation: a fine-regulator of protein function? 'Protein Modifications: beyond the usual suspects' review series. EMBO Rep9:636-641
  53. Bieling P, Kandels-Lewis S, Telley IA, van Dijk J, Janke C, Surrey T (2008) CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites. J Cell Biol183:1223-1233
  54. van Dijk J, Rogowski K, Miro J, Lacroix B, Eddé B, Janke C (2007) A targeted multienzyme mechanism for selective microtubule polyglutamylation. Mol Cell26:437-448
  55. Ikegami K, Heier RL, Taruishi M, Takagi H, Mukai M, Shimma S, Taira S, Hatanaka K, Morone N, Yao I, Campbell PK, Yuasa S, Janke C, Macgregor GR, Setou M (2007) Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function. Proc Natl Acad Sci U S A104:3213–3218
  56. Janke C, Rogowski K, Wloga D, Regnard C, Kajava AV, Strub J-M, Temurak N, van Dijk J, Boucher D, van Dorsselaer A, Suryavanshi S, Gaertig J, Eddé B (2005) Tubulin polyglutamylase enzymes are members of the TTL domain protein family. Science308:1758-1762
  57. Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast21:947-962
  58. Scharfenberger M, Ortiz J, Grau N, Janke C, Schiebel E, Lechner J (2003) Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex. EMBO J22:6584-6597
  59. Regnard C, Fesquet D, Janke C, Boucher D, Desbruyeres E, Koulakoff A, Insina C, Travo P, Eddé B (2003) Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase. J Cell Sci116:4181-4190
  60. Janke C, Martin D, Giraud-Panis M-J, Decoville M, Locker D (2003) Drosophila DSP1 and rat HMGB1 have equivalent DNA binding properties and share a similar secondary fold. J Biochem (Tokyo)133:533-539
  61. Tanaka TU, Rachidi N, Janke C, Pereira G, Galova M, Schiebel E, Stark MJR, Nasmyth K (2002) Evidence that the Ipl1-Sli15 (Aurora Kinase-INCENP) Complex Promotes Chromosome Bi-orientation by Altering Kinetochore-Spindle Pole Connections. Cell108:317-329.
  62. Janke C, Ortiz J, Tanaka TU, Lechner J, Schiebel E (2002) Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. EMBO J21:181-193
  63. Schramm C, Janke C, Schiebel E (2001) Molecular dissection of yeast spindle pole bodies by two hybrid, in vitro binding, and co-purification. Methods Cell Biol67:71-94
  64. Janke C, Ortiz J, Lechner J, Shevchenko A, Shevchenko A, Magiera MM, Schramm C, Schiebel E (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J20:777-791
  65. Holzer M, Rodel L, Seeger G, Gartner U, Narz F, Janke C, Heumann R, Arendt T (2001) Activation of mitogen-activated protein kinase cascade and phosphorylation of cytoskeletal proteins after neurone-specific activation of p21ras. II. Cytoskeletal proteins and dendritic morphology. Neuroscience105:1041-1054
  66. Janke C, Beck M, Holzer M, Bigl V, Arendt T (2000) Analysis of the molecular heterogeneity of the microtubule-associated protein tau by two-dimensional electrophoresis and RT-PCR. Brain Res Brain Res Protoc5:231-242
  67. Janke C, Beck M, Stahl T, Holzer M, Brauer K, Bigl V, Arendt T (1999) Phylogenetic diversity of the expression of the microtubule-associated protein tau: implications for neurodegenerative disorders. Brain Res Mol Brain Res68:119-128
  68. Mack AF, Germer A, Janke C, Reichenbach A (1998) Muller (glial) cells in the teleost retina: consequences of continuous growth. Glia22:306-313
  69. Janke C, Gartner U, Holzer M, Arendt T (1998) Reversible in vivo phosphorylation of tau induced by okadaic acid and by unspecific brain lesion in rat. J Hirnforsch39:143-153
  70. Gärtner U, Janke C, Holzer M, Vanmechelen E, Arendt T (1998) Postmortem changes in the phosphorylation state of tau-protein in the rat brain. Neurobiol Aging19:535-543
  71. Arendt T, Holzer M, Bruckner MK, Janke C, Gartner U (1998) The use of okadaic acid in vivo and the induction of molecular changes typical for Alzheimer's disease. Neuroscience85:1337-1340
  72. Janke C, Holzer M, Klose J, Arendt T (1996) Distribution of isoforms of the microtubule-associated protein tau in grey and white matter areas of human brain: a two-dimensional gelelectrophoretic analysis. FEBS Lett379:222-226