Research Highlight: Three-dimensional spatiotemporal focusing of holographic patterns

ENP group leader Valentina Emiliani and coll. now demonstrate  the experimental achievement of a unique optical system that would enable in combination with other imaging  or functional approaches to photactivate simultaneously cellular- and/or sub-structures in vivo, in several planes in depth . Multiplane generation of spatiotemporally focused light patterns combines temporal focusing, an ultrafast pulse excitation temporaly stretched so that out-of-focus multiphoton excitation is dramatically reduced, to efficient light-sculpting in 3D shapes using computer generated holography using two spatial light modulators to first shape the target light illumination and to second enable axial positioning of the focal plane. The authors apply this optical system to photoconvert in vivo photoactivatable fluorescent protein(Kaede)-expressing neurons in zebrafish larvae enabling cellular resolution of group of separated neurons in different planes. 

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Three-dimensional spatiotemporal focusing of holographic patterns. Oscar Hernandez, Eirini Papagiakoumou, Dimitrii Tanese, Kevin Fidelin, Claire Wyart & Valentina Emiliani 

Nature Communications, 7:11928 , DOI: 10.1038/ncomms11928

Fig. Simultaneous 3D photoconversion of neural ensembles in the zebrafish brain using temporally focused holographic light generation at axially distinct planes. (collaboration with Claire Wyart, ICM, Paris)