The optic tectum has emerged as a tractable visuomotor transformer, in which anatomical and functional studies can allow a better understanding of how behavior is controlled by neuronal circuits. We are examining the formation and function of the visual system in zebrafish larvae using in vivo time-lapse microscopy and state-of-the-art “connectomic” and “optogenetic” approaches to monitor and perturb neuronal activity. We apply complementary cellular and molecular analyses to dissect this circuit and identify the neuronal substrate of visual behaviors.
An issue central to the theme of complexity in the dynamics and function of biological networks concerns the inferences that can be made from multiscale measurements ranging from microscopic to macroscopic levels of integration. Our research is based on interdisciplinary approaches ranging from electrophysiology (intracellular sharp and patch recordings, in vivo), network imaging (voltage sensitive dye), psychophysical measurements to functional databasing and phenomenological and computational modeling.
Our lab studies the cellular and molecular mechanisms underlying retinal information processing. For instance, considering contrast enhancement at the photoreceptor terminals, which remains a matter of controversy, we have revisited the GABA hypothesis.