Vision

Neuronal Circuit Development Group

Main field of research: 

Neurogenetics / neurodevelopment

Keywords: 

zebrafish
Optogenetics
behavior
Vision
neural circuits

ENP label: 

2012

Research Center / Institute: 

Institut Curie - Centre de Recherche - Paris/Orsay

Research Unit Code: 

U934 - UMR3215

The optic tectum has emerged as a tractable visuomotor transformer, in which anatomical and functional studies can allow a better understanding of how behavior is controlled by neuronal circuits. We are examining the formation and function of the visual system in zebrafish larvae using in vivo time-lapse microscopy and state-of-the-art “connectomic” and “optogenetic” approaches to monitor and perturb neuronal activity. We apply complementary cellular and molecular analyses to dissect this circuit and identify the neuronal substrate of visual behaviors.

Leader

Leader: 

Personal

ENP Students: 

Team members: 

Karine Duroure
Thomas Auer
Christoph Gebhardt
Vincenzo Di Donato
Flavia De Santis
Celine Revenu
Shahad Albadri
Noe Testa
Institutions

Principal Institution: 

Institut Curie

Affiliated institutions: 

CNRS
Inserm

University: 

Université Pierre et Marie Curie

Doctoral School: 

ED158
Laboratory

Surname: 

Edith Heard

Initiatives d'Excellence: 

Labex DEEP
Publications

Publications: 

Dunn, T. W., Gebhardt, C., Naumann, E. A., Riegler, C., Ahrens, M. B., Engert, F., and Del Bene, F. Neural Circuits Underlying Visually Evoked Escapes in Larval Zebrafish. Neuron (2016)

Di Donato, V., De Santis, F., Auer, T. O., Testa, N., Sanchez-Iranzo, H., Mercader, N., Concordet, J. P., and Del Bene, F.  2C-Cas9: a versatile tool for clonal analysis of gene function. Genome research (2016)

Auer, T. O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J. P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., and Del Bene, F. Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLife (2015)

Auer, T. O., Duroure, K., Concordet, J. P., and Del Bene, F. CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish. Nature protocols (2014)

Auer, T. O., Duroure, K., De Cian, A., Concordet, J. P., and Del Bene, F.  Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome research (2014)

 

Cognisciences: Synaptic integration and functional plasticity in primary visual cortex

Main field of research: 

Neurophysiology / systems neuroscience

Keywords: 

Vision
electrophysiology
imaging
Multiscale
Modeling

ENP label: 

2007

Research Center / Institute: 

Unité de Neuroscience, Information et Complexité

Research Unit Code: 

UPR 3293

An issue central to the theme of complexity in the dynamics and function of biological networks concerns the inferences that can be made from multiscale measurements ranging from microscopic to macroscopic levels of integration. Our research is based on interdisciplinary approaches ranging from electrophysiology (intracellular sharp and patch recordings, in vivo), network imaging (voltage sensitive dye), psychophysical measurements to functional databasing and phenomenological and computational modeling.

Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

Université Paris Sud

University: 

Université Pierre et Marie Curie

Doctoral School: 

ED158 - 3C
Laboratory

Initiatives d'Excellence: 

Idex i-Code, Idex NeuroSaclay
Publications

Publications: 

Gerard-Mercier F, Carelli PV, Pananceau M, Troncoso XG, Frégnac Y. Synaptic Correlates of Low-Level Perception in V1. J Neurosci. 2016 Apr 6;36(14):3925-42. doi: 10.1523/JNEUROSCI.4492-15.2016.

Fournier J, Monier C, Levy M, Marre O, Sári K, Kisvárday ZF, Frégnac Y. Hidden complexity of synaptic receptive fields in cat V1. J Neurosci. 2014 Apr 16;34(16):5515-28. doi: 10.1523/JNEUROSCI.0474-13.2014.

Arduin P.J. , Ego-Stengel V. , Frégnac Y. and Shulz D.E. (2013) Master neurons induced by operant conditioning in rat motor cortex during a brain-machine interface task. The Journal of Neuroscience. 33(19): 8308-20.

Levy M. , Fournier J. and Frégnac Y. (2013) The role of delayed suppression in slow and fast contrast adaptation in v1 simple cells. The Journal of Neuroscience. 33(15):6388-400.

El Boustani S. , Yger P. , Destexhe A. and Frégnac Y. (2012) A. Stable learning in stochastic network states. J. Neurosci. 32: 194-214.

Frégnac Y. (2012) Reading out the synaptic echoes of low level perception in V1. Lecture Notes in Computer Science. 7583: 486-495.

Chavane F. , Sharon D. , Jancke D. , Marre O. , Frégnac Y. and Grinvald A. (2011) Lateral spread of orientation selectivity in V1 is controlled by intracortical cooperativity. Frontiers in System Neuroscience, 5:4. 1-26.

Retinal information processing : pharmacology and pathologies

Main field of research: 

Neurophysiology / systems neuroscience

Keywords: 

Vision
Retina
therapy
rehabilitation
blind

ENP label: 

2012

Research Center / Institute: 

Institut de la Vision

Research Unit Code: 

UMRS 968 - UMR 7210 - UM 80

Our lab studies the cellular and molecular mechanisms underlying retinal information processing. For instance, considering contrast enhancement at the photoreceptor terminals, which remains a matter of controversy, we have revisited the GABA hypothesis.

Leader

Leader: 

Institutions

Principal Institution: 

Inserm

Affiliated institutions: 

Université Pierre et Marie Curie
CNRS
Laboratory

Initiatives d'Excellence: 

Labex Lifesenses
Publications

Publications: 

Hébert C, Cottance M, Degardin J, Scorsone E, Rousseau L, Lissorgues G, Bergonzo P, Picaud S. Monitoring the evolution of boron doped porous diamond electrode on flexible retinal implant by OCT and in vivo impedance spectroscopy. Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:77-84. doi: 10.1016/j.msec.2016.06.032. Epub 2016 Jun 10.

Froger N, Moutsimilli L, Cadetti L, Jammoul F, Wang QP, Fan Y, Gaucher D, Rosolen SG, Neveux N, Cynober L, Sahel JA, Picaud S. Taurine: the comeback of a neutraceutical in the prevention of retinal degenerations. Prog Retin Eye Res. 2014 Jul;41:44-63. doi: 10.1016/j.preteyeres.2014.03.001. Epub 2014 Apr 8.

Rosolen SG, Neveux N, Sahel J A, Picaud S. Froger N IntEvaluation of the taurine concentrations in dog plasma and aqueous humour: a pilot study. Adv Exp Med Biol. (2013) 775: 145-54.

Linderholm P, Guyomard JL, Djilas M, Salzmann J, Simonutti M, Sahel JA, Safran AB, Renaud P, Picaud S. Long-term in-vivo impedance changes of subretinal microelectrodes implanted in dystrophic P23H rats. International Journal of Artificial Organs (2013) DOI:10.5301/ijao.5000213.

Amel Bendali, Lucas H. Hess, Max Seifert, Valerie Forster, Anne-Fleur Stephan, Jose A. Garrido, Serge Picaud. Purified Neurons can Survive on Peptide-Free Graphene Layers. Adv. Health. Mater. (2013) 0.1002/adhm.201200347.

Lorach H., Benosman R., Marre O., Ieng S.H., Sahel J., Picaud S. Artificial retina : the multichannel processing of the mammalian retina achieved with a neuromorphic asynchronous light acquisition device. Journal of Neural Engineering (2012) 9(6):066004.

Froger N, Cadetti L, Lorach H, Martins J, Bemelmans A, Dubus E, Dégardin J, Pain D, Forster V, Chicaud L, Ivkovic I, Simonutti M, Fouquet S, Jammoul F, Léveillard T, Benosman R, Sahel J A, Picaud S. Taurine provides neuroprotection against retinal ganglion cell degeneration. Plos One (2012) 9(6):066004.