neurogenesis

Cilia biology and neurogenesis

Keywords: 

Cell culture
Histology
neurogenesis
cilia
centriole/centrosome

ENP label: 

2008

Research Unit Code: 

UMR 8197 - U1024

Our goal is to unravel the cellular and molecular mechanisms of neural stem cell biology. Neural stem cells are self-renewing multipotent progenitors, which can be used to replace neurons in degenerated or diseased nervous systems. However, many questions must be answered before neuronal replacement therapies using endogenous precursors become a reality. In particular, it is still unknown how the stem cell integrates the multiple signals from the environment that are responsible for its division, differentiation and survival.

Personal

Team members: 

Adel Al Jord
Nathalie Delgéhyr
Marion Faucourt
Philippe Foerster
Gloria Gonzalez Curto
Alice Meunier
Alexia Mahuzier
Institutions

Principal Institution: 

Ecole Normale Supérieure

Affiliated institutions: 

Inserm
CNRS

University: 

Paris Descartes

Doctoral School: 

ED3C
Laboratory

Surname: 

Institut de Biologie de l'ENS IBENS
Publications

Publications: 

  • · N. Spassky, Y.-G. Han, A. Aguilar, L. Strehl, L. Besse, C. Laclef, M. Romaguera Ros, J.M. Garcia-Verdugo and A. Alvarez-Buylla. (2008) Primary Cilia are required for cerebellar development and Shh-dependent expansion of progenitor pool. Dev Biol, 317 (1) : 246-59.
  • · Y.-G. Han, N. Spassky, M. Romaguera Rosa, J.-M. Garcia-Verdugo, A. Aguilar, S. Schneider-Maunoury and A. Alvarez-Buylla. (2008) Hedgehog signaling and primary cilia are required for the formation of adult neural stem cells. Nat Neurosci, 11(3):277-84
  • · D. Delaunay, K. Heydon, A. Cumano, M. H. Schwab, JL. Thomas, U. Suter, KA. Nave, B. Zalc and N. Spassky. (2008) Early neuronal and glial fate restriction of embryonic neural stem cells. J. Neurosci, 28(10):2551-62 (COVER).
  • B. Guirao, A. Meunier, S. Mortaud, A. Aguilar, J.M. Corsi, L. Strehl, Y. Hirota, A. Desoeuvre, C. Boutin, Y.G. Han, Z. Mirzadeh, H. Cremer, M. Montcouquiol, K. Sawamoto, and N. Spassky. (2010). Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia. Nat Cell Biol, 12(4) :341-50.
  • Y. Hirota, A. Meunier, S. Huang, T. Shimozawa, O. Yamada, Y.S. Kida, M. Inoue, T. Ito, H. Kato, M. Sakaguchi, T. Sunabori, M.A. Nakaya, S. Nonaka, T. Ogura, H. Higuchi, H. Okano, N. Spassky, and K. Sawamoto. (2010). Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II. Development 137(18):3037-46.
  • · G. Keryer, JR. Pineda, G. Liot, J. Kim, P. Dietrich, C. Benstaali, K. Smith, FP. Cordelières, N. Spassky, RJ. Ferrante, I. Dragatsis, and F. Saudou. (2011). Ciliogenesis is regulated by a huntingtin-HAP1-PCM1 pathway and is altered in Huntington disease. J Clin Invest 121(11) :4372-82.
  • · A. Aguilar, A. Meunier, L. Strehl, J. Martinovic, M. Bonniere, T. Attie-Bitach, F. Encha-Razavi and N. Spassky. (2012). Analysis of human samples reveals impaired Shh-dependent cerebellar development in Joubert syndrome/Meckel syndrome. PNAS 109(42) :16951-6.
  • · J.P. Baudoin, L. Viou, P. S. Launay, C. Luccardini, S. Espeso, V. Kiyasova, T. Irinopoulou, C. Alvarez, J.P. Rio, T. Boudier, J.P. Lechaire, N. Kessaris, N. Spassky, and C. Métin (2012). Tangentially migrating neurons assemble a primary cilium that promotes their re-orientation to the cortical plate. Neuron, 76(6) :1108-22.
  • · N. Spassky. (2013). Motile cilia and brain functions : ependymal motile cilia development, organization, function and their associated pathologies. K. Tucker and T. Caspary editors, Springer.
  • · A. Meunier, K. Sawamoto, and N. Spassky. (2013) Ependyma, choroid. Chapter 86 : Comprehensive Developmental Neuroscience. D. Rowitch and A. Alvarez-Buylla editors. Elsevier.
  • · M.B. Grau, G. Gonzalez Curto, C. Rocha, M.M. Magiera, P. Marques, T. Giordano, N. Spassky and C. Janke. (2013) Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia. J Cell Biol 202(3) :441-51.



Zebrafish Neurogenetics (ZEN)

Main field of research: 

Neurogenetics / neurodevelopment

Keywords: 

zebrafish
behavior
motivation
neurogenesis
stem cell
telencephalon
quiescence

ENP label: 

2009

Research Center / Institute: 

Institut Pasteur

Research Unit Code: 

UMR3738

The central nervous system (CNS) of vertebrates is a complex arrangement of neurons and glial cells that underlie brain physiology and animal behavior. These cells are set-up in defined numbers at specific locations from neural progenitors or Neural Stem Cells (NSCs), largely during early stages of life. In addition, the maintenance of NSCs in the brain until adulthood is a general phenomenon, likely crucial to late adaptation events. Indeed, defects in adult neurogenesis have been correlated with neurodegenerative and mood-related disorders, and also occur during ageing.

Leader

Leader: 

Personal

ENP Students: 

Team members: 

Alessandro Alunni
Marion Coolen
Institutions

Principal Institution: 

CNRS

University: 

Université Pierre et Marie Curie

Doctoral School: 

ED 515 Complexité du Vivant
Laboratory

Surname: 

Department of Developmental and stem cell Biology

Initiatives d'Excellence: 

Labex Revive
Publications

Publications: 

L. Dirian, S. Galant, M. Coolen, J. Livet, W. Chen, S. Bedu, C. Houart, L. Bally-Cuif* and I. Foucher*. Spatial regionalization and heterochrony in the formation of adult pallial neural stem cells. Dev. Cell 30:123-136 (2014).

G. FurlanV. Cuccioli, N. Vuillemin, L. Dirian, A. Janue Muntasell, M. CoolenN. DrayS. Bedu, C. Houart, E. Beaurepaire, I. Foucher*and L. Bally-Cuif*. Life-long neurogenic activity of individual neural stem cells and continuous growth establish an outside-in architecture in the teleost pallium. Curr. Biol., in press (2017). * co-senior authorship. 

S. KatzD. Cussigh, N. Urban, F. Guillemot, L. Bally-Cuif* and M. Coolen*. A non-canonical nuclear role for microRNA-9 and Argonaute proteins in balancing the quiescent and activated adult neural stem cell state. Cell Reports 17: 1383-1398 (2016).    

S. GalantG. FurlanM. CoolenL. DirianI. Foucher and L. Bally-CuifEmbryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain. Dev Biol. pii: S0012-1606(16)30424-9 (2016).

N. DrayS. Bedu, N. Vuillemin, A. AlunniM. CoolenM. Krecsmarik, W. Supatto, E. Beaurepaire and L. Bally-Cuif. Large-scale live imaging of adult neuro stem cells in their endogenous niche. Development 142: 3592-3600 (2015).

Morphogenesis of the vertebrate brain

Main field of research: 

Neurogenetics / neurodevelopment

Keywords: 

Morphogenèse du système nerveux, neurogénèse, cil primaire, signalisation, axone

ENP label: 

2011

Research Center / Institute: 

Institut de Biologie Paris Seine

Research Unit Code: 

UMR 7622

Our group is interested in the molecular and cellular mechanisms underlying brain morphogenesis in vertebrates, and in understanding how these mechanisms are perturbed in human diseases.

To investigate these processes, we take advantage of two complementary model organisms, the mouse and the zebrafish. We have three main research axes.

1) We study the gene regulatory hierarchies involved in the early subdivision of the neural plate, the future central nervous system, during gastrulation.

Leader

Leader: 

Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

Université Pierre et Marie Curie

University: 

Université Pierre et Marie Curie

Doctoral School: 

ED158
Laboratory

Surname: 

Laboratoire de Biologie du Développement LBD
Publications

Publications: 

Chen C, Stedman A, Havis E, Anselme I, Onichtchouk D, Giudicelli F, Schneider-Maunoury S. Initiation of cyp26a1 Expression in the Zebrafish Anterior Neural Plate by a Novel Cis-Acting Element. PLoS One. 2016 Mar 9;11(3):e0150639. doi: 10.1371/journal.pone.0150639. eCollection 2016.

Breau MA, Schneider-Maunoury S. Cranial placodes: Models for exploring the multi-facets of cell adhesion in epithelial rearrangement, collective migration and neuronal movements. Dev Biol. 2014 Dec 23. pii: S0012-1606(14)00638-1. doi: 10.1016/j.ydbio.2014.12.012. 

Burcklé C., Gaudé H-M., Vesque C., Silbermann F., Salomon R., Jeanpierre C., Antignac C., Saunier S. and Schneider-Maunoury S. 2011. « Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros ». Human Molecular Genetics 20 :2611-2627. doi: 10.1093/hmg/ddr164.

Besse L., Neti M., Anselme I., Gerhardt C., Rüther U., Laclef C., and Schneider-Maunoury S. 2011. « Primary cilia control telencephalic patterning and morphogenesis via Gli3 proteolytic processing ». Development  138:2079-88.

Stedman A, Lecaudey V, Havis E, Anselme I, Wassef M, Gilardi-Hebenstreit P, Schneider-Maunoury S. 2009. A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3. Dev Biol. 327:566-77.

 Han YG, Spassky N, Romaguera-Ros M, Garcia-Verdugo JM, Aguilar A, Schneider-Maunoury S, Alvarez-Buylla A. 2008. Hedgehog signaling and primary cilia are required for the formation of adult neural stem cells.  Nat Neurosci. 11:277-84.

DelousM, BaalaL, SalomonR, Laclef C, VierkottenJ, ToryK, GolzioC, LacosteT, BesseL, OzilouC, MoutkineI, HellmanNE, AnselmeI, SilbermannF, Vesque C,(…) Schneider-Maunoury S, Attié-Bitach T and Saunier S.2007. The novel ciliary gene FTM is mutated in cerebello-oculo-renal syndrome (Joubert syndrome type B) and Meckel syndrome. Nature Genetics, 39 p. 875-881. News and Views dans Nature Genetics, 39 p. 818-19.