Neurodegeneration

Genes Circuits Rhythms and Neuropathology

Main field of research: 

Neuropharmacology / cell signaling

Keywords: 

Parkinson’s disease
dopamine
Neurodegeneration
Locomotor aging
Drosophila models

ENP label: 

2016

Research Center / Institute: 

ESPCI Paris Tech -Ecole Supérieure de Physique et de Chimie Industrielles

Research Unit Code: 

UMR 8249

Our research program aims at understanding how the brain controls locomotor activity, and how aging or neurodegenerative pathologies, such as Parkinson’s disease and amyotrophic lateral sclerosis, alter this function and in general brain functioning. We try to identify neuronal networks controlling locomotor activity and circadian mechanisms that modulate them in health and disease. Our approach combines genetics methods, brain imaging and behavioral assays.

Leader

Leader: 

Personal

Team members: 

André Klarsfeld
Baya Chérif-Zahar
Ali Mteyrek
Abdul-Raouf Issa
Alexandra Vaccaro
Mathilde Lecompte
Jun Sun
Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

ESPCI

University: 

Université Pierre et Marie Curie

Doctoral School: 

ED 158 3C
Laboratory

Surname: 

Laboratoire Plasticité du cerveau

Initiatives d'Excellence: 

Labex MemoLife, Idex PSL
Publications

Publications: 

Cassar M.#, Issa A.-R.#, Riemensperger T.#, Petitgas C.#, Rival T.#, Coulom H.#, Iché-Torres M.#, Han K.-A. & Birman S#. (2015)A dopamine receptor contributes to paraquat-induced neurotoxicity in Drosophila. Hum. Mol. Genet. 24(1):197-212

Lavista-Llanos S., Svatoš A., Kai M., Riemensperger T.#, Birman S.#, Stensmyr M. C. & Hansson B. S. (2014) Dopamine drivesDrosophila sechellia adaptation to its toxic host. eLife Dec 9;3

Riemensperger T.#, Issa A.-R.#, Pech U., Coulom H.#, Nguy?n M.-V.#, Cassar M.#, Jacquet M.#, Fiala A. & Birman S#. (2013) Asingle dopamine pathway underlies progressive locomotor deficits in a Drosophila model of Parkinson disease. Cell Rep. 5:952–960

Pech U., Pooryasin A., Birman S#. & Fiala A. (2013) Visualization of the connections between Kenyon cells and aminergic neuronsin the Drosophila melanogaster brain using split-GFP reconstitution. J. Comp. Neurol. 521(17):3992-4026

Reinhardt A., Feuillette S., Cassar M.#, Callens C.#, Thomassin H., Birman S.#, Lecourtois M., Antoniewski C. & Tricoire H. (2012)Lack of miRNA misregulation at early pathological stages in Drosophila neurodegenerative disease models. Front. Genet. 3:226

Regulation of microtubule dynamics and functions

Main field of research: 

Neurogenetics / neurodevelopment

Keywords: 

Neurodegeneration
tubulin code
microtubule cytoskeleton
posttranslational modifications
neuronal differentiation

ENP label: 

2015

Research Center / Institute: 

Institut Curie - Centre de Recherche - Paris/Orsay

Research Unit Code: 

UMR3348

Microtubules are key cytoskeletal elements involved in a large number of functions in eukaryotic cells. They assemble from a protein dimer of a- and b-tubulin, two highly similar and conserved proteins. Tubulins are subject to a large variety of posttranslational modifications, which provide a rapid and reversible mechanism to diversify microtubule functions in cells. Our team is studying the mechanisms and functional roles of these modifications by using an interdisciplinary approach.

Leader

Leader: 

Personal

Team members: 

Annemarie Wehenkel
Cecilia Serieyssol
Kathiresan Natarajan
Laurence Vaslin
Maria Magiera
Ngoc-Lan Julie Nguyen
Sudarshan Gadadhar
Tiziana Giordano
Lurlene Akendengue
Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

Institut Curie
Université Paris Sud 11

University: 

Université Paris Saclay / Université Paris Descartes

Doctoral School: 

SDSV / FdV
Laboratory

Initiatives d'Excellence: 

Labex CelTisPhyBio, contrat ANR, équipe FRM
Publications

Publications: 

Gadadhar S, Dadi H, Bodakuntla S, Schnitzler A, Bieche I, Rusconi F, Janke C (2017) Tubulin glycylation controls primary cilia length. J Cell Biol 216: 2701-2713

Barisic M, Silva e Sousa R, Tripathy SK, Magiera MM, Zaytsev AV, Pereira AL, Janke C, Grishchuk EL, Maiato H (2015) Microtubule detyrosination guides chromosomes during mitosis. Science 348: 799-803

Nirschl JJ, Magiera MM, Lazarus JE, Janke C, Holzbaur ELF (2016) alpha-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons. Cell Rep: celrep 2509

Belvindrah R, Natarajan K, Shabajee P, Bruel-Jungerman E, Bernard J, Goutierre M, Moutkine I, Jaglin XH, Savariradjane M, Irinopoulou T, Poncer J-C, Janke C, Francis F (2017) Mutation of the alpha-tubulin Tuba1a leads to straighter microtubules and perturbs neuronal migration. J Cell Biol 216: 2443-2461

Bosch Grau M, Masson C, Gadadhar S, Rocha C, Tort O, Marques Sousa P, Vacher S, Bieche I, Janke C (2017) Alterations in the balance of tubulin glycylation and glutamylation in photoreceptors leads to retinal degeneration. J Cell Sci 130: 938-949

 

Membrane Transport

Main field of research: 

Neuropharmacology / cell signaling

Keywords: 

Optogenetics
Transporter
Synaptic vesicle
Lysosome
Neurodegeneration

ENP label: 

2014

Research Center / Institute: 

INCC - UMR 8002 - Paris Descartes University

Research Unit Code: 

UMR 8250

RESEARCH INTERESTS:

Leader

Leader: 

Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

Université Paris Descartes
Laboratory

Surname: 

Neurophotonics laboratory
Publications

Publications: 

Chapel A, Kieffer-Jaquinod S, Sagné C#, Verdon Q#, Ivaldi C, Mellal M, Thirion J, Jadot M, Bruley C, Garin J, Gasnier B#, Journet  A (2013) An extended proteome map of the lysosomal membrane reveals novel potential transporters. Mol Cell Proteomics, 12:1572

M. Martineau, T. Shi, J. Puyal, A.M. Knolhoff, J. Dulong, B. Gasnier#, J. Klingauf, J. V. Sweedler, R. Jahn, and J-P Mothet (2013) Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci, 34:646.

A. Jezegou#, E. Llinares, C. Anne#, S. Kieffer-Jaquinod, S. O'Regan#, J. Aupetit , A. Chabli, C. Sagné#, C. Debacker#, B. Chadefaux-Vekemans, A. Journet, B. Andre, B. Gasnier# (2012) Heptahelical protein PQLC2 is a lysosomal cationic amino acid exporter underlying the action of cysteamine in cystinosis therapy. PNAS 109:E3434

R. Ruivo#, G.C. Bellenchi#, X. Chen#, G. Zifarelli, C. Sagné#, C. Debacker#, M. Pusch, S. Supplisson, B. Gasnier# (2012) Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin, PNAS 109:E210.

N. Pietrancosta*, C. Anne*#, H. Prescher, R. Ruivo#, C. Sagné#, C. Debacker#, H.O. Bertrand, R. Brossmer, F. Acher, B. Gasnier# (2012) Successful prediction of the substrate-binding pocket in the SLC17 transporter sialin, JBC 287:11489