Biophysics

Advanced optics for Neuroscience

Keywords: 

electrophysiology
Biophysics
Sciences Cognitives

ENP label: 

2010

Research Unit Code: 

UMR 8118

Transmission at glutamate synapses in the cerebellar cortex: biophysical, pharmacological and photochemical approaches.

Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

Université Paris Descartes
Laboratory

Surname: 

Laboratoire de physiologie cérébrale
Publications

Publications: 

Trigo FF, Sakaba T, Ogden D, Marty A. (2012) Readily releasable pool of synaptic vesicles measured at single synaptic contacts. Proc Natl Acad Sci U S A. 109(44):18138-43. doi: 10.1073/pnas.1209798109.

Palma-Cerda F, Auger C, Crawford DJ, Hodgson AC, Reynolds SJ, Cowell JK, Swift KA, Cais O, Vyklicky L, Corrie JE, Ogden D. (2012) New caged neurotransmitter analogs selective for glutamate receptor sub-types based on methoxynitroindoline and nitrophenylethoxycarbonyl caging groups. Neuropharmacology. 63(4):624-34.

Anselmi F, Ventalon C, Bègue A, Ogden D, Emiliani V. (2011) Three-dimensional imaging and photostimulation by remote-focusing and holographic light patterning. Proc Natl Acad Sci U S A.;108(49):19504-9.

Papageorgiou G, Beato M, Ogden D (2011) Synthesis and photolytic evaluation of a nitroindoline-caged glycine with a side chain of high negative charge for use in neuroscience. Tetrahedron 67: 5228-5234

Auger C, Ogden D. (2010) AMPA receptor activation controls type I metabotropic glutamate receptor signalling via a tyrosine kinase at parallel fibre-Purkinje cell synapses. J Physiol. 588(Pt 16):3063-74

Genetics and Physiology of Hearing

Main field of research: 

Neurogenetics / neurodevelopment

Keywords: 

human genetics
electrophysiology
Biophysics
Biochemistry
Hearing molecular physiology
Sensorineural deafness
Retinal defects (Usher syndrome)
Cell biology

ENP label: 

2010

Research Center / Institute: 

Institut Pasteur

Research Unit Code: 

UMRS 1120

Our research projects have two tightly linked goals: (i) to decipher the cellular and molecular mechanisms that underlie the development of the auditory system, the way it processes acoustic signals (mechanotransduction (MET), and the synaptic properties of the auditory sensory cells and their afferents; and (ii) to identify the genes and pathogenic pathways causing deafness in humans in early- and late-onset forms, as well as forms accompanied by retinal defects (Usher syndrome). We also search for therapeutic tools based on these findings.

Leader

Leader: 

Institutions

Principal Institution: 

Inserm

Affiliated institutions: 

Collège de France

University: 

Université Pierre et Marie Curie
Laboratory

Initiatives d'Excellence: 

Labex Lifesenses
Publications

Publications: 

Delmaghani S, Aghaie A, Bouyacoub Y, El Hachmi H, Bonnet C, Riahi Z, Chardenoux S, Perfettini I, Hardelin JP, Houmeida A, Herbomel P, Petit C. Mutations in CDC14A, Encoding a Protein Phosphatase Involved in Hair Cell Ciliogenesis, Cause Autosomal-Recessive Severe to Profound Deafness. J Cell Biol. 2016 Jan 18;212(2):231-44. doi: 10.1083/jcb.201509017. Epub 2016 Jan 11.

Avan P, Büki B, Petit C (2013) Auditory distortions: origins and functions. Physiol Rev 93, 1563?1619.

Bonnet C, ? Petit C, Marlin S (2013) Biallelic nonsense mutations in the otogelin-like gene (OTOGL) in a child affected by mild to moderate hearing impairment. Gene 527, 537-40.

Kamiya K, Michel V, Giraudet F, Riederer B, Foucher I, Papal S, Perfettini I, Le Gal S, Verpy E, Xia W, Seidler U, Georgescu MM, Avan P, El-Amraoui A, Petit C. An unusually powerful mode of low-frequency sound interference due to defective hair bundles of the auditory outer hair cells. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9307-12. doi: 10.1073/pnas.1405322111. Epub 2014 Jun 11.

Boulay A-C, ? Petit C, Avan P, Cohen-Salmon M (2013) Hearing is normal without connexin30. J Neurosci 33, 430-34.

El-Amraoui A, Petit C (2013) Cadherin defects in inherited human diseases. Progr Mol Biol Transl Sci, Conn PM (ed.), Elsevier 116, 361-84.

Greenspan R, Petit C (2013) Neurogenetics. Curr Opin Neurobiol. 23, 1-2.

Lepelletier L, ? Petit C (2013) Auditory hair cell centrioles undergo confined brownian motion throughout the developmental migration of the kinocilium. Biophys J 105, 48-58.

Membrane Traffic in Healthy & Diseased Brain

Main field of research: 

Neurogenetics / neurodevelopment

Keywords: 

mouse genetics
Exocytosis
SNARE
Biophysics
Microscopy

ENP label: 

2007

Research Center / Institute: 

Institut de Psychiatrie et Neurosciences de Paris, IPNP (ex-CPN) - Centre Hospitalier Sainte Anne

Research Unit Code: 

UMRS 894

Membrane trafficking allows for the communication between the different membrane compartments of the biosynthetic and endocytic pathways and for the communication between cells and their environment through the secretion of signalling molecules by exocytosis and capture of nutrients by endocytosis. Exocytosis and endocytosis are crucial to maintain cell homeostasis and are also involved in differentiation and morphogenesis of cells.

Leader

Leader: 

Personal

ENP Students: 

Team members: 

Thierry Galli, DR1 INSERM, DU
Christian Vannier, DR2 INSERM
Lydia Danglot, CR1 INSERM
David Tareste, CR1 INSERM
Sébastien Nola, IR2 INSERM
Agathe Verraes, TCS CNRS
Jose Wojnacki, Postdoc
Guan Wang, doc.
Ahmed Zahraoui, DR CNRS
Institutions

Principal Institution: 

Inserm

Affiliated institutions: 

Sainte Anne

University: 

Université Paris Descartes
Laboratory

Initiatives d'Excellence: 

Labex ‘WhoAmI ?’ Investissement d’Avenir, Plateforme Distribuée ‘FranceBioImaging-FBI’
Publications

Publications: 

Petkovic M, Jemaiel A, Daste F, Specht CG, Izeddin I, Vorkel D, Verbavatz JM, Darzacq X, Triller A, Pfenninger KH, Tareste D, Jackson CL, Galli T. The SNARE Sec22b has a non-fusogenic function in plasma membrane expansion. Nat Cell Biol. 2014 May;16(5):434-44. doi: 10.1038/ncb2937. Epub 2014 Apr 6.

Larghi P, Williamson DJ, Carpier JM, Dogniaux S, Chemin K, Bohineust A, Danglot L, Gaus K, Galli T§, Hivroz C§. (2013) VAMP7 controls T cell activation by regulating the recruitment and phosphorylation of vesicular Lat at TCR-activation sites. Nat Immunol. doi:10.1038/ni.2609. § co-senior authors. (F1000)

Burgo A., Proux-Gillardeaux, V., Sotirakis, E., Bun, P., Casano,A., Verraes, A., Liem, R., Formstecher, E., Coppey, M. Galli, T. (2012). A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery. Developmental Cell,23:166?180.

Zylbersztejn K, Petkovic M, Burgo A, Deck M, Garel S, Marcos S, Bloch-Gallego E, Nothias F, Serini G, Bagnard D, Binz T, Galli T. (2012). The vesicular SNARE Synaptobrevin is required for Semaphorin 3A axonal repulsion. J Cell Biol 196:37-46. (F1000)

Danglot L*, Zylbersztejn K*, Petkovic M*, Meziane H, Combe R, Champy Mf, Birling Mc, Pavlovic G, Bizot Jc, Trovero F, Della Ragione F, Proux-Gillardeaux V, Sorg T, D?esposito M, Galli T. (2012). Absence of TI-VAMP/Vamp7 leads to increased anxiety in mice. J Neurosci 32:1962-1968 (F1000)

Danglot L, Chaineau M, Dahan M, Gendron M-C, Boggetto N, Perez F, and Galli T. (2010). Role of TI-VAMP and CD82 in EGF receptor cell surface dynamics and signaling. J Cell Sci 123:723-35.