Wavefront-engineering microscopy

Research center

45 rue des Saints Pères
75006 Paris
Daniel Zytnicki

Institution

CNRS
Université Paris Descartes
Université Paris Descartes

Laboratory

Neurophotonics laboratory
UMR 8250
Available to host a PhD student

Publications

Szabo V, Ventalon C, De Sars V, Bradley J, Emiliani V. Spatially selective holographic photoactivation and functional fluorescence imaging in freely behaving mice with a fiberscope. Neuron. 2014 Dec 17;84(6):1157-69. doi: 10.1016/j.neuron.2014.11.005. Epub 2014 Nov 26.

Lauterbach MA, Guillon M, Soltani A, Emiliani V. STED microscope with spiral phase contrast. Sci Rep. 2013;3:2050. doi: 10.1038/srep02050.

Hernandez O, Papagiakoumou E, Tanese D, Fidelin K, Wyart C, Emiliani Three-dimensional spatiotemporal focusing of holographic patterns. V.Nat Commun. 2016 Jun 16;7:11928. doi: 10.1038/ncomms11928.

Lauterbach MA, Guillon M, Soltani A, Emiliani V. STED microscope with spiral phase contrast. Sci Rep. 2013;3:2050. doi: 10.1038/srep02050.

Functional patterned multiphoton excitation deep inside scattering tissue, E. Papagiakoumou, A. Bègue, B. Leshem, O. Schwartz, B. Stell, J. Bradley, D. Oron and V. Emiliani
Nature Photonics (in press)


Emergence of population bursts from simultaneous activation of  small subsets of preBötzinger Complex inspiratory neurons  K. Kam, J.W. Worrell1, C. Ventalon, V. Emiliani, and J. L. Feldman
Journal of Neuroscience 33: 3332-3338 (2013)

LCoS nematic SLM characterization and modeling for diffraction efficiency optimization, zero and ghost orders suppression, 
E. Ronzitti, M. Guillon, V. de Sars, and V. Emiliani.
Optics Express Vol. 20, Iss. 16, pp. 17843–17855 (2012)

Reshaping the optical dimension in optogenetics
A. Vaziri, and V. Emiliani
Current Opinion in Neurobiology 22, 128-137 (2012)

Two-photon Optogenetics
D. Oron, E. Papagiakoumou, F. Anselmi and V. Emiliani,
Progress in Brain Research 194: 119-143 (2012)

3D imaging and photostimulation by remote focusing and holographic light patterning
F. Anselmi*, C. Ventalon*, V. DeSars, D. Ogden and V. Emiliani
PNAS 108, 19504-19509 (2011)

3D Holographic Photostimulation of the Dendritic Arbor
S. Yang, E. Papagiakoumou, M. Guillon, V. de Sars, C.-M Tang, and V. Emiliani
Journal of Neural engineering 8: 046002 (2011)


Scanless two-photon excitation of channelrhodopsin-2, E. Papagiakoumou, F. Anselmi, A. Begue, V. de Sars, J. Gluckstad, E. Y. Isacoff, and V. Emiliani Nature Methods 7, 848-854 (2010).

Holographic photolysis for multiple cell stimulation in mouse hippocampal slices, M. Zahid, M. Velez-Fort, E. Papagiakoumou, C. Ventalon, M. C. Angulo, and V. Emiliani PLoS One 5, e9431 (2010).

Temporal focusing with spatially modulated excitation.E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron Optics Express 17, 5391-5401 (2009).

Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses, E. Papagiakoumou, C. Lutz, V. De Sars, D. Oron, and V. Emiliani, Optics Express 16, 22039-22047 (2008).

Holographic photolysis of caged neurotransmittersC. Lutz, T. Otis, V. DeSars, S. Charpak, D. Digregorio, and V. Emiliani, Nature Methods 5, 821-827 (2008).

Fields of research

Neurophysiology / systems neuroscience

Research Theme

This research team is dedicated to the development of optical techniques based on wave front engineering microscopy.Traditional optical systems modify excitation light patterns through the use of lenses, diaphragms, curved mirrors, gratings, or optical fibers. They are typically limited to the generation of simple patterns (generally of circular symmetry) which are relatively difficult to change during the course of an experiment. In contrast the use of active optical elements such as micro-mirror and optical membrane devices, acousto-optical or nematic liquid crystals displays permits laser light to be dynamically redistributed by modulation of its phase or amplitude.

The active nature of these devices allows illumination patterns to be rapidly and conveniently adapted to experimental needs and greatly widens the types of experiments and studies that can be performed on biological systems.The interest of this team is to develop advanced methods for wave front engineering and to exploit the potentiality of wave front engineering microscopy in the field of neuroscience.

The team activity is organized in three research lines: 1. Spatiotemporal control of neuronal activity by holographic photoactivation patterns.2. Super resolution microscopy (STED)3. Microendoscopy for deep imaging.