Interactions between neurons and oligodendroglia in myelination and myelin repair

Research center

Institution

Inserm
Université Paris Descartes
ED157 GC2ID

Laboratory

UMRS 894
Phone: (+33) 1 70 64 99 35
UMRS 894

Keywords

Patch clamp
Optogenetics
Available to host a PhD student

Publications

Balia M*, Vélez-Fort M*, Passlick S, Schäfer C, Audinat E, Steinhäuser C, Seifert G, Angulo MC (2015) Postnatal down-regulation of the GABAA receptor 2 subunit in neocortical NG2 cells accompanies synaptic-to-extrasynaptic switch in GABAergic transmission mode. Cereb Cortex, 25(4):1114-23

Balia M, Benamer N, Angulo MC (2017) A specific GABAergic synapse onto oligodendrocyte precursors does not regulate cortical oligodendrogenesis. Glia. 65(11):1821-1832.

Ledonne F, Orduz D, Mercier J, Vigier L, Grove EA, Tissir F, Angulo MC, Pierani A*, Coppola E*. (2016) Targeted inactivation of Bax reveals subtype-specific mechanism of Cajal-Retzius neuron death in the postnatal cerebral cortex. Cell Rep. 17(12) 3133

Wake H*, Ortiz FC*, Woo DH, Lee P, Angulo MC, Fields D (2015) Non-synaptic junctions on myelinating glia promote preferential myelination of electrically-active axons. Nat Commun 6:7844

Orduz D*, Maldonado PP*, Balia M, Vélez-Fort M, de Sars V, Yanagawa Y, Emiliani V, Angulo MC (2015) Interneurons and oligodendrocyte progenitors form a structured synaptic network in the developing neocortex. eLife 4:e06953

Fields of research

Neurophysiology / systems neuroscience

Research Theme

Oligodendrocyte precursor cells expressing the chondroitin sulfate proteoglycan NG2, also called NG2 cells, have the ability to proliferate in the postnatal brain to generate oligodendrocytes in grey and white matters. NG2 cells play a critical role in myelination during postnatal brain development, but a pool of these progenitors is maintained in the adult and recruited to lesions in demyelinating diseases. Recent discoveries have demonstrated that NG2 cells are contacted by functional glutamatergic and GABAergic synapses from neurons in grey and white matters. The function of these synapses is still elusive. Our group is interested in the functional properties and role of neuron-NG2 cell synapses as well as in exploring new neuronal signaling mechanisms controlling NG2 cell activity in the normal and injured brains. Our research program consists of studying these synapses in the somatosensory cortex (grey matter) and corpus callosum (white matter) to dissect the synaptic connectivity of NG2 cells and decipher whether neuronal synaptic inputs control NG2 cell fate in an activity-dependent manner. We use a multidisciplinary approach that combines patch-clamp recordings, calcium imaging, immunostaining techniques, optogenetics and advanced optical methods to analyze NG2 cell physiology in acute slices and in vivo during postnatal development and a demyelination/remyelination process. Our studies may bring new perspectives on the roles played by NG2 cells in the brain and for the design of innovative therapies promoting myelin repair in demyelinating diseases.

ENP Students

Paloma MALDONADO