Inhibitory transmission

Research center

45 rue d’Ulm
75230 Paris
Marc Mézard

Institution

Ecole Normale Supérieure
Inserm
CNRS
ED158
Université Pierre et Marie Curie

Laboratory

Institut de Biologie de l'ENS IBENS
U1024 - UMR8197
Labex Memolife, IDEX PSL*

Keywords

Cerebellum
Optogenetics
Vesicular loading
Glycine
Multiphoton imaging
Inhibitory transmission
Available to host a PhD student

Publications

Husson Z, Rousseau CV, Broll I, Zeilhofer HU, Dieudonné S. Differential GABAergic and glycinergic inputs of inhibitory interneurons and Purkinje cells to principal cells of the cerebellar nuclei. J Neurosci. 2014 Jul 9;34(28):9418-31. doi: 10.1523/JNEUROSCI.0401-14.2014.

Otsu Y, Marcaggi P, Feltz A, Isope P, Kollo M, Nusser Z, Mathieu B, Kano M, Tsujita M, Sakimura K, Dieudonné S. Activity-dependent gating of calcium spikes by A-type K+ channels controls climbing fiber signaling in Purkinje cell dendrites.Neuron. 2014 Oct 1;84(1):137-51. doi: 10.1016/j.neuron.2014.08.035. Epub 2014 Sep 11.

in : Co-Existence and Co-Release of Classical Neurotransmitters (Gutierrez R, ed) : Springer.Electrical coupling mediates tunable low-frequency oscillations and resonance in the cerebellar Golgi cell networkDugué GP, Brunel N, Hakim V, Schwartz E, Chat M, Lévesque M, Courtemanche R, Léna C and Dieudonné S (2009)Neuron 61:126-139

Functional coupling between mGluR1 and Cav3.1 T-type calcium channels contributes to parallel fiber-induced fast calcium signaling within Purkinje cell dendritic spines.Hildebrand ME, Isope P, Miyazaki T, Nakaya T, Garcia E, Feltz A, Schneider T, Hescheler J, Kano M, Sakimura K, Watanabe M, Dieudonné S, Snutch TP, (2009)The Journal of neuroscience : the official journal of the Society for Neuroscience

Striatal progenitors derived from human ES cells mature into DARPP32 neurons in vitro and in quinolinic acid-lesioned rats.Aubry L, Bugi A, Lefort N, Rousseau F, Peschanski M and Perrier AL (2008)Proc Natl Acad Sci U S A 105:16707-16717

The glycine transporter GlyT2 controls the dynamics of synaptic vesicle refilling in inhibitory spinal cord neurons.Rousseau F, Aubrey KR and Supplisson S (2008)J Neurosci 28:9755-9768.

Postsynaptic determinants of inhibitory transmission at mixed GABAergic/glycinergic synapses.Dieudonné S and Diana MA (2008)

Fields of research

Neurophysiology / systems neuroscience

Research Theme

The aim of the group is to understand how inhibitory neurons control the gain and shape the temporal output of neuronal assemblies. To this end we combine electrophysiological recordings, optical recordings and the latest optogenetic tools. More specifically, we study the function of inhibitory neurons in the cerebellum and in thalamic-projecting pathways at the single-cell and network levels. Fast multipoint multiphoton imaging of calcium influx in the Purkinje cell, a giant inhibitory neuron. In the vertebrate hindbrain, inhibitory synaptic transmission is mediated by the neurotransmitters glycine and GABA, which can be released by separate neurons or co-released by mixed inhibitory neurons.

We have shown that postsynaptic cells are able to select the neurotransmitter they use by expressing the appropriate GABAA or glycine receptors at the synapse. We are now exploring how this mechanism may be used during the homeostatic regulation of inhibition.

Team members

MARCAGGI Païkan