Oligodendroglial, microglial interactions during demyelination and remyelination in a Xenopus transgenic model
Dates:September 18, 2017 - June 29, 2018
Application deadline:June 29, 2018
~ Sept-Dec 2017
~ Jan-March 2018
~ April-June 2018
We have generated a transgenic Xenopus of conditional demyelination in which myelinating oligodendrocytes express the GFP reporter. We are now using the Crispr/Cas9 knock-in approach to introduce the tdTomato fluorescent reporter into the microglial lineage of this transgenic Xenopus line. Fluorescent microglial cells (red) and oligodendrocytes (green) will allow us to monitor in vivo, thanks to the transparency of tadpoles, microglial activation following demyelination and remyelination.
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