UMR 8250

Membrane Transport

Main field of research: 

Neuropharmacology / cell signaling

Keywords: 

Optogenetics
Transporter
Synaptic vesicle
Lysosome
Neurodegeneration

ENP label: 

2014

Research Center / Institute: 

INCC - UMR 8002 - Paris Descartes University

Research Unit Code: 

UMR 8250

RESEARCH INTERESTS:

Leader

Leader: 

Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

Université Paris Descartes
Laboratory

Surname: 

Neurophotonics laboratory
Publications

Publications: 

Chapel A, Kieffer-Jaquinod S, Sagné C#, Verdon Q#, Ivaldi C, Mellal M, Thirion J, Jadot M, Bruley C, Garin J, Gasnier B#, Journet  A (2013) An extended proteome map of the lysosomal membrane reveals novel potential transporters. Mol Cell Proteomics, 12:1572

M. Martineau, T. Shi, J. Puyal, A.M. Knolhoff, J. Dulong, B. Gasnier#, J. Klingauf, J. V. Sweedler, R. Jahn, and J-P Mothet (2013) Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci, 34:646.

A. Jezegou#, E. Llinares, C. Anne#, S. Kieffer-Jaquinod, S. O'Regan#, J. Aupetit , A. Chabli, C. Sagné#, C. Debacker#, B. Chadefaux-Vekemans, A. Journet, B. Andre, B. Gasnier# (2012) Heptahelical protein PQLC2 is a lysosomal cationic amino acid exporter underlying the action of cysteamine in cystinosis therapy. PNAS 109:E3434

R. Ruivo#, G.C. Bellenchi#, X. Chen#, G. Zifarelli, C. Sagné#, C. Debacker#, M. Pusch, S. Supplisson, B. Gasnier# (2012) Mechanism of proton/substrate coupling in the heptahelical lysosomal transporter cystinosin, PNAS 109:E210.

N. Pietrancosta*, C. Anne*#, H. Prescher, R. Ruivo#, C. Sagné#, C. Debacker#, H.O. Bertrand, R. Brossmer, F. Acher, B. Gasnier# (2012) Successful prediction of the substrate-binding pocket in the SLC17 transporter sialin, JBC 287:11489

Wavefront-engineering microscopy

Main field of research: 

Neurophysiology / systems neuroscience

ENP label: 

2007

Research Center / Institute: 

Institut de la Vision

Research Unit Code: 

UMR 8250

This research team is dedicated to the development of optical techniques based on wave front engineering microscopy.Traditional optical systems modify excitation light patterns through the use of lenses, diaphragms, curved mirrors, gratings, or optical fibers. They are typically limited to the generation of simple patterns (generally of circular symmetry) which are relatively difficult to change during the course of an experiment.

Leader

Leader: 

Institutions

Principal Institution: 

CNRS

Affiliated institutions: 

Université Paris Descartes

University: 

Université Paris Descartes
Laboratory

Surname: 

Neurophotonics laboratory
Publications

Publications: 

Szabo V, Ventalon C, De Sars V, Bradley J, Emiliani V. Spatially selective holographic photoactivation and functional fluorescence imaging in freely behaving mice with a fiberscope. Neuron. 2014 Dec 17;84(6):1157-69. doi: 10.1016/j.neuron.2014.11.005. Epub 2014 Nov 26.

Lauterbach MA, Guillon M, Soltani A, Emiliani V. STED microscope with spiral phase contrast. Sci Rep. 2013;3:2050. doi: 10.1038/srep02050.

Hernandez O, Papagiakoumou E, Tanese D, Fidelin K, Wyart C, Emiliani Three-dimensional spatiotemporal focusing of holographic patterns. V.Nat Commun. 2016 Jun 16;7:11928. doi: 10.1038/ncomms11928.

Lauterbach MA, Guillon M, Soltani A, Emiliani V. STED microscope with spiral phase contrast. Sci Rep. 2013;3:2050. doi: 10.1038/srep02050.

Functional patterned multiphoton excitation deep inside scattering tissue, E. Papagiakoumou, A. Bègue, B. Leshem, O. Schwartz, B. Stell, J. Bradley, D. Oron and V. Emiliani
Nature Photonics (in press)


Emergence of population bursts from simultaneous activation of  small subsets of preBötzinger Complex inspiratory neurons  K. Kam, J.W. Worrell1, C. Ventalon, V. Emiliani, and J. L. Feldman
Journal of Neuroscience 33: 3332-3338 (2013)

LCoS nematic SLM characterization and modeling for diffraction efficiency optimization, zero and ghost orders suppression, 
E. Ronzitti, M. Guillon, V. de Sars, and V. Emiliani.
Optics Express Vol. 20, Iss. 16, pp. 17843–17855 (2012)

Reshaping the optical dimension in optogenetics
A. Vaziri, and V. Emiliani
Current Opinion in Neurobiology 22, 128-137 (2012)

Two-photon Optogenetics
D. Oron, E. Papagiakoumou, F. Anselmi and V. Emiliani,
Progress in Brain Research 194: 119-143 (2012)

3D imaging and photostimulation by remote focusing and holographic light patterning
F. Anselmi*, C. Ventalon*, V. DeSars, D. Ogden and V. Emiliani
PNAS 108, 19504-19509 (2011)

3D Holographic Photostimulation of the Dendritic Arbor
S. Yang, E. Papagiakoumou, M. Guillon, V. de Sars, C.-M Tang, and V. Emiliani
Journal of Neural engineering 8: 046002 (2011)


Scanless two-photon excitation of channelrhodopsin-2, E. Papagiakoumou, F. Anselmi, A. Begue, V. de Sars, J. Gluckstad, E. Y. Isacoff, and V. Emiliani Nature Methods 7, 848-854 (2010).

Holographic photolysis for multiple cell stimulation in mouse hippocampal slices, M. Zahid, M. Velez-Fort, E. Papagiakoumou, C. Ventalon, M. C. Angulo, and V. Emiliani PLoS One 5, e9431 (2010).

Temporal focusing with spatially modulated excitation.E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron Optics Express 17, 5391-5401 (2009).

Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses, E. Papagiakoumou, C. Lutz, V. De Sars, D. Oron, and V. Emiliani, Optics Express 16, 22039-22047 (2008).

Holographic photolysis of caged neurotransmittersC. Lutz, T. Otis, V. DeSars, S. Charpak, D. Digregorio, and V. Emiliani, Nature Methods 5, 821-827 (2008).