UMR 8237

Neuroimaging and behavior of the zebrafish

Main field of research: 

Neurophysiology / systems neuroscience

Keywords: 

light-sheet microscopy
neuroimaging
zebrafish
sensory-motor integration
circuit inference

ENP label: 

2016

Research Center / Institute: 

Laboratoire Jean Perrin

Research Unit Code: 

UMR 8237

The overall objective of the group is to unravel principles of neural computations underlying sensory-motor integration in thevertebrate brain. We use the zebrafish larva as a model since it currently constitutes the only vertebrate system amenable towhole-brain recording with cellular resolution. Using one- or two-photon light-sheet microscopy, we are able to monitor the long-termactivity of the quasi-entirety of the 100,000 neurons that comprise the animal brain, as it performs basic sensory-motor tasks.

Leader

Leader: 

Personal

Team members: 

Raphaël Candelier
Sergei Kruglik
Sébastien Wolf
Alexis Dubreuil
Sophia Karpenko
Guillaume Le Goc
Institutions

Principal Institution: 

UPMC

Affiliated institutions: 

CNRS

University: 

Université Pierre et Marie Curie

Doctoral School: 

ED 564
Publications

Publications: 

Whole-brain functional imaging with two-photon light-sheet microscopy, S Wolf, W. Supatto, G. Debrégeas, P. Mahou, S. G Kruglik,J-M. Sintes, E. Beaurepaire, R. Candelier Nature Methods 12, 379–380 (2015)

An Amplitude Modulation/Demodulation Scheme for Whisker-Based Texture Perception, Y. Boubenec, L. N. Claverie, D. E. Shulz,G. Debrégeas, J. Neurosci. 34(33): 10832-10843 (2014)

Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy. T. Panier,S. Romano, R. Olive, T. Pietri, G. sumbre, R. Candelier, G. Debrégeas, Frontiers in Neural Circuits 7, 0065 (2013)

The role of fingerprints in the coding of tactile information probed with a biomimetic sensor, J. Scheibert, S. Leurent, A. Prevost et G. Debrégeas, Science 323 1503-1506 (2009). Reviewed in Science news, Science Update (radio), Science signaling, Nature news, Nature Research highlights, Nature Methods,Analytical Chemistry, Atten. Percept & Psych.

Signatures of granular microstructure in dense shear flows. D. Mueth, G. Debregeas, G. Karczmar, P. Eng, S. Nagel & H. Jaeger,Nature 406, 385-388 (2000)

Multisensory signal processing: From whole-brain activity to behavior

Main field of research: 

Neurophysiology / systems neuroscience

Keywords: 

light-sheet microscopy
zebrafish
multisensory signal processing
whole-brain calcium imaging

ENP label: 

2016

Research Center / Institute: 

Laboratoire Jean Perrin

Research Unit Code: 

UMR 8237

Our brain needs to constantly fuse sensory information detected by our multiple senses in order to produce a seamless coherent representation of the world. Rather than being the exception, this binding process is ubiquitous to sensory-motor integration and is implicated in most cognitive functions. Its impairment is a cause of various pathologies, such as schizophrenia or autism. Multisensory processing operates on all brain levels from primary cortices over subcortical structures up to higher associative centers, while the smallest operational units are single multisensory neurons.

Leader

Leader: 

Personal

Team members: 

Thomas Panier (PostDoc)
Kris Severi (PostDoc)
Geoffrey Migault (PhD)
Hugo Trentenseaux (PhD)
Matthieu Dujany (Master)
Louis Chevalier (Master)
Institutions

Principal Institution: 

Sorbonne Université

Affiliated institutions: 

CNRS

University: 

UPMC

Doctoral School: 

ED 564
Laboratory

Surname: 

Bormuth
Publications

Publications: 

 Seclected Publications:

1. Migault G., van der Plas T.L.  , Trentesaux H. , Panier T., Candelier R., R. Proville , B. Englitz , Debrégeas G., Bormuth, V.  Whole-Brain Calcium Imaging during Physiological Vestibular Stimulation in Larval Zebrafish

Current Biology 28, 1-13 (2018)

2. Wolf S., Dubreuil A., Bertoni T., Böhm U.L., Bormuth V., Candelier R., Karpenko S., Hildebrand D.G.C. , Bianco I. H., Monasson R., Debrégeas G.     Sensorimotor computation underlying phototaxis in zebrafish.

Nature Communication 8, 651 (2017) 

3. Olive R., Wolf S., Dubreuil A., Bormuth V., Debregeas G., Candelier R.    Rheotaxis of Larval Zebrafish: Behavioral Study of a Multi-Sensory Process.

Frontiers in System Neuroscience 10, UNSP 14 (2016)

4. Bormuth, V., Barral, J., Joanny, J.F., Jülicher, F. & Martin, P.     Transduction channels gating controls friction on vibrating hair-cell bundles in the ear.

PNAS 111, 7185-90 (2014)

5. Gardner, M.K.*, Zanic, M.*, Gell, C., Bormuth, V., & Howard J.     Depolymerizing Kinesins Kip3 and MCAK Shape Cellular Microtubule Architecture by Differential Control of Catastrophe.

CELL 147, 1092-1103 (2011)  

6. Bormuth, V., Varga, V., Howard, J., & Schäffer, E.     Protein friction limits diffusive and directed movements of kinesin motors on microtubules.

SCIENCE 325, 870 - 873 (2009)  

7. Varga, V.*, Leduc, C.*, Bormuth, V., Diez, S., & Howard, J.     Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization.

CELL 138, 1174-83 (2009)  

8. Bormuth, V.*, Otsu, Y.*, et al.     Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope.

J. Neurosci. Methods 173(2), 259-270 (2008)